confocal laser scanning microscopy
Recently Published Documents


TOTAL DOCUMENTS

1331
(FIVE YEARS 156)

H-INDEX

61
(FIVE YEARS 6)

2022 ◽  
Author(s):  
Pranita Mhaske ◽  
Stefan Kasapis ◽  
Asgar Farahnaky ◽  
Mina Dokouhaki

AbstractThere is an increasing demand for the design of complex bio-composites with customized structural characteristics for use in processed food products. Phase behaviour of these mixtures determines textural properties, encouraging the pursue of a rapid technique that can accurately quantify it. The present work tests the efficacy of confocal laser scanning microscopy (CLSM) coupled with image analysis software (Imaris), for the quantification of phase behaviour in complex tertiary systems. In doing so, it develops phase separated gels of agarose and gelatin supporting inclusions of canola oil. The polysaccharide was replaced with whey protein isolate (WPI) and the topology of the tertiary dispersion with gelatin and canola oil was also examined. Reproducible phase volume estimates were obtained, including those of the lipid phase, which were a close match to the actual concentrations added to the hydrocolloid gel. The approach could offer an alternative to the rheological estimation, via theoretical blending law analysis, of phase volumes in bio-composites. Graphical Abstract


2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Elena Temereva ◽  
Nadezhda Rimskaya-Korsakova ◽  
Vyacheslav Dyachuk

AbstractThe Oweniidae are marine annelids with many unusual features of organ system, development, morphology, and ultrastructure. Together with magelonids, oweniids have been placed within the Palaeoannelida, a sister group to all remaining annelids. The study of this group may increase our understanding of the early evolution of annelids (including their radiation and diversification). In the current research, the morphology and ulta-anatomy of the head region of Owenia borealis is studied by scanning electron microscopy (SEM), 3D reconstructions, transmission electron microscopy (TEM), and whole-mount immunostaining with confocal laser scanning microscopy. According to SEM, the tentacle apparatus consists of 8–14 branched arms, which are covered by monociliary cells that form a ciliary groove extending along the oral side of the arm base. Each tentacle contains a coelomic cavity with a network of blood capillaries. Monociliary myoepithelial cells of the tentacle coelomic cavity form both the longitudinal and the transverse muscles. The structure of this myoepithelium is intermediate between a simple and pseudo-stratified myoepithelium. Overall, tentacles lack prominent zonality, i.e., co-localization of ciliary zones, neurite bundles, and muscles. This organization, which indicates a non-specialized tentacle crown in O. borealis and other oweniids with tentacles, may be ancestral for annelids. TEM, light, and confocal laser scanning microscopy revealed that the head region contains the anterior nerve center comprising of outer and inner (=circumoral) nerve rings. Both nerve rings are organized as concentrated nerve plexus, which contains perikarya and neurites extending between basal projections of epithelial cells (radial glia). The outer nerve ring gives rise to several thick neurite bundles, which branch and extend along aboral side of each tentacle. Accordingly to their immunoreactivity, both rings of the anterior nerve center could be homologized with the dorsal roots of circumesophageal connectives of the typical annelids. Accordingly to its ultrastructure, the outer nerve ring of O. borealis and so-called brain of other oweniids can not be regarded as a typical brain, i.e. the most anterior ganglion, because it lacks ganglionic structure.


2021 ◽  
Vol 105 (1) ◽  
pp. 159-166
Author(s):  
Ladislav Chladil ◽  
Hana Hálová ◽  
Ondřej Čech

Confocal Laser Scanning Microscopy (CLSM) is a widely used technique mainly in fields of biology or multidisciplinary material sciences. Although CLSM has the ability to monitor also electrochemical processes like lead sulfate-crystal growth, nobody used CLSM for such application. We performed operando observation of the pasted active mass of negative electrode for lead-acid batteries during deep cycling. Electrode with pasted negative active mass was optimized for cycling in ECC-opto-std electrochemical cell by EL-CELL. Lead sulfate crystal growth and changes of electrode surface during cycling were observed using a laser scanning confocal microscope Olympus Lext OLS4100. We evaluate the surface changes and sulfate crystal growth. The cycling mode leads to fast gradual degradation of the negative electrode and massive growth of lead sulfate crystals. Confocal laser scanning microscopy was identified as a powerful technique for visualization of lead sulfate crystal promotion during battery cycling.


Sign in / Sign up

Export Citation Format

Share Document