Modification of C-type inactivating Shaker potassium channels by chloramine-T

1996 ◽  
Vol 431 (4) ◽  
pp. 483-493 ◽  
Author(s):  
Thomas Schlief ◽  
Roland Schönherr ◽  
S. H. Heinemann
Keyword(s):  
Potassium Channels ◽  
Chloramine T ◽  
Shaker Potassium Channels

scholarly journals Probing the Cavity of the Slow Inactivated Conformation of Shaker Potassium Channels

2007 ◽  
Vol 129 (5) ◽  
pp. 403-418 ◽  
Author(s):  
Gyorgy Panyi ◽  
Carol Deutsch
Keyword(s):  
Potassium Channels ◽  
Binding Site ◽  
Conformational Change ◽  
Marked Decrease ◽  
Selectivity Filter ◽  
Slow Inactivation ◽  
Voltage Gated ◽  
Activation Gate ◽  
Shaker Potassium Channels

Slow inactivation involves a local rearrangement of the outer mouth of voltage-gated potassium channels, but nothing is known regarding rearrangements in the cavity between the activation gate and the selectivity filter. We now report that the cavity undergoes a conformational change in the slow-inactivated state. This change is manifest as altered accessibility of residues facing the aqueous cavity and as a marked decrease in the affinity of tetraethylammonium for its internal binding site. These findings have implications for global alterations of the channel during slow inactivation and putative coupling between activation and slow-inactivation gates.

scholarly journals Key Residues in the Interface between Voltage Sensor and Pore Domain in Shaker Potassium Channels

2018 ◽  
Vol 114 (3) ◽  
pp. 476a
Author(s):  
João L. Carvalho-de-Souza ◽  
Francisco Bezanilla
Keyword(s):  
Potassium Channels ◽  
Voltage Sensor ◽  
Key Residues ◽  
Pore Domain ◽  
Shaker Potassium Channels

Mechanisms of the inhibition of Shaker potassium channels by protons

2003 ◽  
Vol 447 (1) ◽  
pp. 44-54 ◽  
Author(s):  
John G. Starkus ◽  
Zoltan Varga ◽  
Roland Sch�nherr ◽  
Stefan H. Heinemann
Keyword(s):  
Potassium Channels ◽  
Shaker Potassium Channels

scholarly journals Cross Talk between Activation and Slow Inactivation Gates of Shaker Potassium Channels

2006 ◽  
Vol 128 (5) ◽  
pp. 547-559 ◽  
Author(s):  
Gyorgy Panyi ◽  
Carol Deutsch
Keyword(s):  
Potassium Channels ◽  
Cross Talk ◽  
Cysteine Residue ◽  
Slow Inactivation ◽  
Fundamental Factor ◽  
Functional Assays ◽  
Activation Gate ◽  
The Status ◽  
Shaker Potassium Channels ◽  
Rate Of Activation

This study addresses the energetic coupling between the activation and slow inactivation gates of Shaker potassium channels. To track the status of the activation gate in inactivated channels that are nonconducting, we used two functional assays: the accessibility of a cysteine residue engineered into the protein lining the pore cavity (V474C) and the liberation by depolarization of a Cs+ ion trapped behind the closed activation gate. We determined that the rate of activation gate movement depends on the state of the inactivation gate. A closed inactivation gate favors faster opening and slower closing of the activation gate. We also show that hyperpolarization closes the activation gate long before a channel recovers from inactivation. Because activation and slow inactivation are ubiquitous gating processes in potassium channels, the cross talk between them is likely to be a fundamental factor in controlling ion flux across membranes.

Modification of C-type inactivatingShaker potassium channels by chloramine-T

1996 ◽  
Vol 431 (4) ◽  
pp. 483-493 ◽  
Author(s):  
Thomas Schlief ◽  
Roland Schönherr ◽  
Stefan H. Heinemann
Keyword(s):  
Potassium Channels ◽  
Chloramine T

scholarly journals External barium influences the gating charge movement of Shaker potassium channels

1997 ◽  
Vol 72 (1) ◽  
pp. 77-84 ◽  
Author(s):  
R.S. Hurst ◽  
M.J. Roux ◽  
L. Toro ◽  
E. Stefani
Keyword(s):  
Potassium Channels ◽  
Charge Movement ◽  
Gating Charge ◽  
Shaker Potassium Channels

scholarly journals Generalized Interaction Energy Analysis of Intersubunit Linkage in Shaker Potassium Channels

2014 ◽  
Vol 106 (2) ◽  
pp. 742a
Author(s):  
Sandipan Chowdhury ◽  
Benjamin M. Haehnel ◽  
Baron Chanda
Keyword(s):  
Potassium Channels ◽  
Interaction Energy ◽  
Energy Analysis ◽  
Shaker Potassium Channels

scholarly journals Function of Shaker Potassium Channels Produced by Cell-Free Translation Upon Injection into Xenopus Oocytes

2013 ◽  
Vol 104 (2) ◽  
pp. 197a
Author(s):  
Brian W. Jarecki ◽  
Shin-ichi Makino ◽  
Emily T. Beebe ◽  
Brian G. Fox ◽  
Baron Chanda
Keyword(s):  
Potassium Channels ◽  
Xenopus Oocytes ◽  
Free Translation ◽  
Shaker Potassium Channels
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