In vitro effects of Crotalus durissus terrificus and Bothrops jararaca venoms on Giardia duodenalis trophozoites

2005 ◽  
Vol 98 (4) ◽  
pp. 339-344 ◽  
Author(s):  
Lina Shinohara ◽  
Stella Fellipe de Freitas ◽  
Reinaldo José da Silva ◽  
Semíramis Guimarães
Keyword(s):  
Giardia Duodenalis ◽  
Crotalus Durissus Terrificus ◽  
Bothrops Jararaca ◽  
In Vitro Effects ◽  
Crotalus Durissus

In vitro effects of propolis on Giardia duodenalis trophozoites

Phytomedicine ◽  
2006 ◽  
Vol 13 (3) ◽  
pp. 170-175 ◽  
Author(s):  
S.F. Freitas ◽  
L. Shinohara ◽  
J.M. Sforcin ◽  
S. Guimarães
Keyword(s):  
Giardia Duodenalis ◽  
In Vitro Effects

scholarly journals In vivo and in vitro prospection of the anti-ophidic properties exercised by the extracts of Jacaranda decurrens L.

2021 ◽  
Vol 43 ◽  
pp. e57016
Author(s):  
Marcus Vinícius Cardoso Trento ◽  
Mateus Santos Carapiá ◽  
Pedro Henrique Souza César ◽  
Mariana Aparecida Braga ◽  
Andreimar Martins Soares ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Local Effects ◽  
Crotalus Durissus Terrificus ◽  
Mechanism Of Inhibition ◽  
Local Toxicity ◽  
Sds Page ◽  
Crotalus Durissus ◽  
Venom Proteins

The research and development of alternative treatments for snakebites (e.g., medicinal plants) is necessary due to the high costs of the existing ones. The effects of the aqueous extracts from Jacaranda decurrens leaves, roots, and xylopodium were analyzed upon the venom-induced (Bothrops spp. and Crotalus spp.) systemic and local toxicity. The extracts were able to partially inhibit the phospholipase activity of the venoms from Bothrops jararacussu and Crotalus durissus terrificus. The myotoxic, edema-inducing, coagulant, and hemorrhagic activities were also inhibited. The SDS-PAGE showed that the venom proteins were intact after their incubation with the extracts. This suggests that the possible mechanism of inhibition is not related to the degradation of the protein but rather to their binding to specific sites of the enzymes. The extracts significantly prolonged the survival time of animals in the lethality assay performed with Crotalus durissus terrificus venom and its toxin (crotoxin). The anti-ophidic activity of medicinal plants may aid in the management of snakebites in distant locations by reducing the victim’s local effects and time to heal.

scholarly journals Inhibition of proteases and phospholipases A2 from Bothrops atrox and Crotalus durissus terrificus snake venoms by ascorbic acid, vitamin E, and B-complex vitamins

2016 ◽  
Vol 88 (3 suppl) ◽  
pp. 2005-2016 ◽  
Author(s):  
CARLOS H.M. OLIVEIRA ◽  
ANDERSON A. SIMÃO ◽  
MARCUS V.C. TRENTO ◽  
PEDRO H.S. CÉSAR ◽  
SILVANA MARCUSSI
Keyword(s):  
Vitamin E ◽  
Low Cost ◽  
Crotalus Durissus Terrificus ◽  
Phospholipases A2 ◽  
Antioxidant Mechanism ◽  
Valuable Adjunct ◽  
B Complex Vitamins ◽  
Crotalus Durissus ◽  
Bothrops Atrox

ABSTRACT The enzyme inhibition by natural and/ or low-cost compounds may represent a valuable adjunct to traditional serotherapy performed in cases of snakebite, mainly with a view to mitigate the local effects of envenoming. The objective of this study was to evaluate possible interactions between vitamins and enzymes that comprise Bothrops atrox and Crotalus durissus terrificus venoms, in vitro. Proteolysis inhibition assays (substrates: azocasein, collagen, gelatin and fibrinogen), hemolysis, coagulation, hemagglutination were carried out using different proportions of vitamins in face of to inhibit minimum effective dose of each venom. The vitamins were responsible for reducing 100% of breaking azocasein by C.d.t. venom, thrombolysis induced by B. atrox and fibrinogenolysis induced by both venoms. It is suggested the presence of interactions between vitamin and the active site of enzymes, for example the interactions between hydrophobic regions present in the enzymes and vitamin E, as well as the inhibitions exercised by antioxidant mechanism.

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