The mating type locus of Neurospora crassa: identification of an adjacent gene and characterization of transcripts surrounding the idiomorphs

1998 ◽  
Vol 259 (6) ◽  
pp. 615-621 ◽  
Author(s):  
T. A. Randall ◽  
R. L. Metzenberg
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Adjacent Gene ◽  
Type Locus ◽  
Mating Type Locus

A SEARCH FOR COMPLEXITY AT THE MATING-TYPE LOCUS OF NEUROSPORA CRASSA

1973 ◽  
Vol 15 (3) ◽  
pp. 577-585 ◽  
Author(s):  
Dorothy Newmeyer ◽  
H. Branch Howe Jr. ◽  
Donna R. Galeazzi
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Adjacent Gene ◽  
Opposite Mating Type ◽  
Type Locus ◽  
Linked Gene ◽  
Heterokaryon Incompatibility ◽  
Mating Type Locus ◽  
Incompatibility Reaction ◽  
The Right

Evidence for complexity at the mating-type locus of Neurospora crassa was sought by selecting recombinants between closely linked markers on either side. All recombinants were tested for crossing ability, to test the hypothesis that the two mating-type alleles are actually closely linked self-sterile mutants; such tests should also detect subunits analogous to the α and β subunits of the A factor of Schizophyllum or Coprinus. No change in crossing ability was found among the 5,019 recombinants tested, representing 235,000 viable ascospores. The results indicate that if subunits exist, they are not more than 0.002 units apart. Twelve hundred and forty of the recombinants were tested in a way that should also have detected subunits analogous to the A and B factors of Schizophyllum and Coprinus, except that A and B would be closely linked. No such subunits were detected.N. crassa strains of opposite mating type are heterokaryon-incompatible during vegetative growth, and observations of various investigators have suggested that the heterokaryon incompatibility might be controlled by a separate closely-linked gene rather than by mating type itself. A sample of the recombinants was therefore tested for separation of the heterokaryon-incompatibility and crossing-compatibility functions. (Heterokaryon-incompatibility was scored by the presence of an incompatibility reaction in duplications heterozygous for mating type; this technique is simple and eliminates complications due to unlinked heterokaryon-incompatibility loci, several of which are known in N. crassa.) No separation was found. The results indicate that if an adjacent gene is responsible for the heterokaryon-incompatibility, it is not more than 0.0078 units from mating type, if on the left, and not more than 0.018 units from mating type, if on the right.

scholarly journals A Second Gene Controlling Allelic Recombination in Neurospora Crassa

1966 ◽  
Vol 19 (6) ◽  
pp. 1039 ◽  
Author(s):  
DG Catoheside
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus

Experiments to determine whether rec-l, which increases allelic recombinationat the his-l locus in Neurospora crassa, also affects the am locus disclosed anothergene, rec-3. It appears that ree-l is specific to his-l and that ree-3 is specific to am, inthe sense that his-l is insensitive to rec-3 and am is insensitive to ree-l. The locus of rec-l is 18�9 units from the am his-l region; rec-3 is linked either to the am his-l region or to the mating type locus and 12�1 units from the relevant region.

Cloning of the A Mating-Type Locus from Lepista nuda and Characterization of Its Genetic Structure

2015 ◽  
Vol 71 (6) ◽  
pp. 669-677 ◽  
Author(s):  
Chengbo Rong ◽  
Shuang Zhao ◽  
Dengjin Li ◽  
Lijuan Wang ◽  
Shouxian Wang ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus

Characterization of mat A-2, mat A-3 and ΔmatA Mating-Type Mutants of Neurospora crassa

Genetics ◽  
1998 ◽  
Vol 148 (3) ◽  
pp. 1069-1079 ◽  
Author(s):  
Adlane V-B Ferreira ◽  
Zhiqiang An ◽  
Robert L Metzenberg ◽  
N Louise Glass
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Sexual Development ◽  
Vegetative Growth ◽  
Double Mutant ◽  
Sexual Cycle ◽  
Wild Type ◽  
Type Locus ◽  
Isolation And Characterization

AbstractThe mating-type locus of Neurospora crassa regulates mating identity and entry into the sexual cycle. The mat A idiomorph encodes three genes, mat A-1, mat A-2, and mat A-3. Mutations in mat A-1 result in strains that have lost mating identity and vegetative incompatibility with mat a strains. A strain containing mutations in both mat A-2 and mat A-3 is able to mate, but forms few ascospores. In this study, we describe the isolation and characterization of a mutant deleted for mat (ΔmatA), as well as mutants in either mat A-2 or mat A-3. The ΔmatA strain is morphologically wild type during vegetative growth, but it is sterile and heterokaryon compatible with both mat A and mat a strains. The mat A-2 and mat A-3 mutants are also normal during vegetative growth, mate as a mat A strain, and produce abundant biparental asci in crosses with mat a, and are thus indistinguishable from a wild-type mat A strain. These data and the fact that the mat A-2 mat A-3 double mutant makes few asci with ascospores indicate that MAT A-2 and MAT A-3 are redundant and may function in the same pathway. Analysis of the expression of two genes (sdv-1 and sdv-4) in the various mat mutants suggests that the mat A polypeptides function in concert to regulate the expression of some sexual development genes.

Isolation and characterization of the mating type locus of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana

2007 ◽  
Vol 8 (1) ◽  
pp. 111-120 ◽  
Author(s):  
LAURA CONDE-FERRÁEZ ◽  
CEES WAALWIJK ◽  
BLONDY B. CANTO-CANCHÉ ◽  
GERT H. J. KEMA ◽  
PEDRO W. CROUS ◽  
...  
Keyword(s):  
Mating Type ◽  
Causal Agent ◽  
Mycosphaerella Fijiensis ◽  
Type Locus ◽  
Mating Type Locus ◽  
Isolation And Characterization ◽  
Black Leaf Streak ◽  
Black Leaf Streak Disease

New mutations that suppress mating-type vegetative incompatibility in Neurospora crassa

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Tia S. Vellani ◽  
Anthony J. F. Griffiths ◽  
N. Louise Glass
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Vegetative Incompatibility ◽  
Type Locus ◽  
Regulatory Locus ◽  
Mating Type Locus ◽  
Recessive Mutations ◽  
Incompatibility Locus ◽  
New Alleles ◽  
New Mutations

The mating-type locus in the ascomycete Neurospora crassa functions as a vegetative-incompatibility locus during asexual growth such that A + a heterokaryons and A/a partial diploids are inhibited in their growth. In this study, we sought mutations that suppress mating-type associated vegetative incompatibility by using A/a partial diploids. Mutants were selected as spontaneous escapes from inhibited growth. Suppressors were identified by selecting escapes that retained the capacity to mate with both A and a strains. The escaped partial diploids were then outcrossed to remove the duplication. Seven suppressors were identified that segregated as single, recessive mutations. All seven of the suppressors were shown to be allelic to a previously obtained suppressor, tol, by segregation analyses and heterokaryon tests. This result indicates that all seven mutations are new alleles of tol and suggests that tol is a key regulatory locus in the expression of mating-type associated vegetative incompatibility in N. crassa.Key words: vegetative incompatibility, mating type, suppressors, Neurospora.

Effects of thetolmutation on allelic interactions athetloci inNeurospora crassa

Genome ◽  
1997 ◽  
Vol 40 (6) ◽  
pp. 834-840 ◽  
Author(s):  
John F. Leslie ◽  
Carl T. Yamashiro
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Vegetative Compatibility ◽  
Vegetative Incompatibility ◽  
Type Locus ◽  
Mating Type Locus ◽  
Inneurospora Crassa ◽  
Incompatibility Reactions

A mutant at the tol locus of Neurospora crassa can suppress heterokaryon (vegetative) incompatibility associated with differences at the mating-type locus. We tested the ability of this allele to suppress the vegetative incompatibility reactions that can occur when strains differ at one of nine het loci (het-C, -D, -E, -5, -6, -7, -8, -9, and -10). We found no cases in which the tol mutant suppresses a heteroallelic het locus interaction. Thus, the interaction(s) that leads to vegetative incompatibility because of differences at the mating-type locus is distinct from the interaction(s) that leads to vegetative incompatibility because of differences at any of these nine het loci.Key words: heterokaryon, mating type, vegetative compatibility.

Characterization of the mating-type locus (MAT) reveals a heterothallic mating system inKnightiella splachnirima

2017 ◽  
Vol 49 (4) ◽  
pp. 373-385 ◽  
Author(s):  
Lars R. LUDWIG ◽  
Tina C. SUMMERFIELD ◽  
Janice M. LORD ◽  
Garima SINGH
Keyword(s):  
Mating System ◽  
Sexual Reproduction ◽  
Mating Type ◽  
Conservation Management ◽  
Inverse Pcr ◽  
Type Locus ◽  
Mating Type Locus ◽  
Screening Protocol ◽  
Pcr Techniques

AbstractIn the present study, we characterized the mating-type locus ofKnightiella splachnirima(Icmadophilaceae) using degenerate and inverse PCR techniques. We screened for the presence of both mating-type locus idiomorphs in DNA extracts of minuscule samples of haploid thalline tissue. We found that only one of the two idiomorphs was present in each sample, and the mating-type ratio (MAT1-1:MAT1-2) was very balanced, being 8:10 and 13:14 at local and global scales, respectively. This indicates that the species is probably self-incompatible and requires the presence of compatible mating partners for sexual reproduction (heterothallic mating system). Furthermore, we provide a mating-type screening protocol withK. splachnirimaspecific mating-type locus primers, which could serve as an essential tool for the conservation management of this rare Australasian endemic.

scholarly journals Molecular Characterization of tol, a Mediator of Mating-Type-Associated Vegetative Incompatibility in Neurospora crassa

Genetics ◽  
1999 ◽  
Vol 151 (2) ◽  
pp. 545-555 ◽  
Author(s):  
Patrick Ka Tai Shiu ◽  
N Louise Glass
Keyword(s):  
Sexual Reproduction ◽  
Neurospora Crassa ◽  
Mating Type ◽  
Vegetative Growth ◽  
Point Mutations ◽  
Coiled Coil ◽  
Wild Type ◽  
Opposite Mating Type ◽  
Type Locus

Abstract The mating-type locus in the haploid filamentous fungus, Neurospora crassa, controls mating and sexual development. The fusion of reproductive structures of opposite mating type, A and a, is required to initiate sexual reproduction. However, the fusion of hyphae of opposite mating type during vegetative growth results in growth inhibition and cell death, a process that is mediated by the tol locus. Mutations in tol are recessive and suppress mating-type-associated heterokaryon incompatibility. In this study, we describe the cloning and characterization of tol. The tol gene encodes a putative 1011-amino-acid polypeptide with a coiled-coil domain and a leucine-rich repeat. Both regions are required for tol activity. Repeat-induced point mutations in tol result in mutants that are wild type during vegetative growth and sexual reproduction, but that allow opposite mating-type individuals to form a vigorous heterokaryon. Transcript analyses show that tol mRNA is present during vegetative growth but absent during a cross. These data suggest that tol transcription is repressed to allow the coexistence of opposite mating-type nuclei during the sexual reproductive phase. tol is expressed in a mat A, mat a, A/a partial diploid and in a mating-type deletion strain, indicating that MAT A-1 and MAT a-1 are not absolutely required for transcription or repression of tol. These data suggest that TOL may rather interact with MAT A-1 and/or MAT a-1 (or downstream products) to form a death-triggering complex.

scholarly journals The Neurospora crassa pheromone precursor genes are regulated by the mating type locus and the circadian clock

2002 ◽  
Vol 45 (3) ◽  
pp. 795-804 ◽  
Author(s):  
Piotr Bobrowicz ◽  
Rebecca Pawlak ◽  
Alejandro Correa ◽  
Deborah Bell-Pedersen ◽  
Daniel J. Ebbole
Keyword(s):  
Circadian Clock ◽  
Neurospora Crassa ◽  
Mating Type ◽  
Type Locus ◽  
Mating Type Locus
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