An AFLP marker that differentiates biotypes of the Asian rice gall midge (Orseolia oryzae, Wood-Mason) is sex-linked and also linked to avirulence

2000 ◽  
Vol 263 (2) ◽  
pp. 328-334 ◽  
Author(s):  
S. K. Behura ◽  
S. Nair ◽  
S. C. Sahu ◽  
M. Mohan
Keyword(s):  
Aflp Marker ◽  
Gall Midge ◽  
Orseolia Oryzae ◽  
Asian Rice ◽  
Rice Gall Midge

Phylogeography of the Asian rice gall midge Orseolia oryzae (Wood Mason) (Diptera: Cecidomyiidae) in Thailand

Genetica ◽  
2017 ◽  
Vol 145 (1) ◽  
pp. 37-49 ◽  
Author(s):  
Solene Janique ◽  
Wantana Sriratanasak ◽  
Kulchana Ketsuwan ◽  
Jirapong Jairin ◽  
Ekgachai Jeratthitikul
Keyword(s):  
Gall Midge ◽  
Orseolia Oryzae ◽  
Asian Rice ◽  
Rice Gall Midge

Differentiation of Asian rice gall midge, Orseolia oryzae (Wood-Mason), biotypes by sequence characterized amplified regions (SCARs)

1999 ◽  
Vol 8 (3) ◽  
pp. 391-397 ◽  
Author(s):  
S. K. Behura ◽  
S. C. Sahu ◽  
S. Rajamani ◽  
A. Devi ◽  
R. Mago ◽  
...  
Keyword(s):  
Gall Midge ◽  
Orseolia Oryzae ◽  
Asian Rice ◽  
Rice Gall Midge ◽  
Sequence Characterized Amplified Regions

Polymorphisms flanking the mariner integration sites in the rice gall midge (Orseolia oryzae Wood-Mason) genome are biotype-specific

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 947-954 ◽  
Author(s):  
Susanta K Behura ◽  
Suresh Nair ◽  
Madan Mohan
Keyword(s):  
Integration Site ◽  
Gall Midge ◽  
Insect Pest ◽  
Inverse Pcr ◽  
Sequence Information ◽  
Orseolia Oryzae ◽  
Degenerate Primers ◽  
Asian Rice ◽  
Integration Sites ◽  
Rice Gall Midge

In an effort to study genome diversity within and between the Indian biotypes of the Asian rice gall midge, Orseolia oryzae, a major insect pest of rice, we made use of mariner transposable element integration site polymorphisms. Using degenerate primers, the design of which is based on mariner sequences, we amplified a ca. 450 bp mariner sequence from the rice gall midge. The mariner sequence showed homology with that of a mariner element isolated from the Hessian fly, Mayetiola destructor, a major dipteran pest of wheat. Southern hybridization, using this mariner fragment as a probe, revealed that the mariner elements are moderately to highly repetitive in the rice gall midge genome. Based on the sequence information of this 450-bp PCR-amplified fragment, outward-directed primers were designed and used in an inverse PCR (iPCR) to amplify the DNA flanking the conserved regions. To study the regions flanking the mariner integration sites, we employed a novel PCR-based approach: a combination of sequence specific amplification polymorphism (SSAP) and amplified fragment length polymorphism (AFLP). The outward-directed mariner-specific primer was used in combination with adapter-specific primers with 1–3 selective nucleotides at their 3' ends. The amplification products were resolved on an agarose gel, Southern-transferred onto nylon membranes, and probed with the iPCR fragment. Results revealed biotype-specific polymorphisms in the regions flanking the mariner integration sites, suggesting that mariner elements in the rice gall midge may be fixed in a biotype-specific manner. The implications of these results are discussed in the context of biotype differentiation.Key words: DNA fingerprinting, inverse PCR (iPCR), Oryza sativa, rice pest, transposon.

scholarly journals Isolation and Characterization of Microsatellite Loci in the Asian Rice Gall Midge (Orseolia oryzae) (Diptera: Cecidomyiidae)

2011 ◽  
Vol 12 (1) ◽  
pp. 755-772 ◽  
Author(s):  
Jagadish S. Bentur ◽  
Deepak Kumar Sinha ◽  
Ch. Padmavathy ◽  
Charagonda Revathy ◽  
Mayandi Muthulakshmi ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Gall Midge ◽  
Orseolia Oryzae ◽  
Asian Rice ◽  
Isolation And Characterization ◽  
Rice Gall Midge
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