Genotyping single nucleotide polymorphisms by primer extension and high performance liquid chromatography

1999 ◽  
Vol 104 (1) ◽  
pp. 89-93 ◽  
Author(s):  
Bastiaan Hoogendoorn ◽  
Michael J. Owen ◽  
Peter J. Oefner ◽  
Nigel Williams ◽  
Jehannine Austin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Single Nucleotide Polymorphisms ◽  
High Performance ◽  
Primer Extension ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Application of denaturing high-performance liquid chromatography for mapping of single nucleotide polymorphisms in barley (Hordeum vulgare L.)

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 523-528 ◽  
Author(s):  
Raja Kota ◽  
Markus Wolf ◽  
Wolfgang Michalek ◽  
Andreas Graner
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Hordeum Vulgare ◽  
Dna Sequence ◽  
High Throughput ◽  
Doubled Haploid ◽  
High Performance ◽  
Nucleotide Polymorphisms ◽  
Hordeum Vulgare L ◽  
Single Nucleotide

Recent advances in DNA sequence analysis and the establishment of high-throughput assays have provided the framework for large-scale discovery and analysis of DNA sequence variation. In this context, single nucleotide polymorphisms (SNPs) are of particular interest. To initiate a systematic approach to develop an SNP map of barley (Hordeum vulgare L.), we have employed denaturing high-performance liquid chromatography (DHPLC) to analyse segregating SNP patterns in a doubled-haploid (DH) mapping population. To this end, SNPs between the parental genotypes were identified using a direct sequencing approach. Once a SNP was established between the parents, the optimal melting temperature of the PCR fragment containing the SNP was predicted for its analysis by DHPLC. Following the detection of the optimal temperature, the DH lines were analysed for the presence of either of the alleles. To test the utility of the analysis, data from previously mapped RFLP markers from which these SNPs were derived were compared. Results from these experiments indicate that DHPLC can be efficiently employed in analysing SNPs on a high-throughput scale.Key words: denaturing high performance liquid chromatography, doubled-haploid lines, restriction fragment length polymorphism, genetic mapping, molecular markers.

High‐Performance Sieving Electrophoresis for Single‐Nucleotide Polymorphisms with a Structuring Hydrogel Network

2019 ◽  
Vol 221 (2) ◽  
pp. 1900385
Author(s):  
Qingxiang You ◽  
Ping Wang ◽  
Dawei Zhang ◽  
Zhenqing Li ◽  
Yoshinori Yamaguchi
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Performance ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hydrogel Network
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