Efficient production of (R)-(−)-2-hydroxy-4-phenylbutyric acid by recombinant Pichia pastoris expressing engineered d-lactate dehydrogenase from Lactobacillus plantarum with a single-site mutation

2018 ◽  
Vol 41 (9) ◽  
pp. 1383-1390 ◽  
Author(s):  
Xiaoru Wang ◽  
Zhituo Yu ◽  
Jiawei Tang ◽  
Dong Yi ◽  
Shaoxin Chen
Keyword(s):  
Lactate Dehydrogenase ◽  
Pichia Pastoris ◽  
Lactobacillus Plantarum ◽  
Single Site ◽  
Efficient Production ◽  
Site Mutation ◽  
Recombinant Pichia Pastoris ◽  
Phenylbutyric Acid

scholarly journals Efficient Production of Optically Pure d-Lactic Acid from Raw Corn Starch by Using a Genetically Modified l-Lactate Dehydrogenase Gene-Deficient and α-Amylase-Secreting Lactobacillus plantarum Strain

2008 ◽  
Vol 75 (2) ◽  
pp. 462-467 ◽  
Author(s):  
Kenji Okano ◽  
Qiao Zhang ◽  
Satoru Shinkawa ◽  
Shogo Yoshida ◽  
Tsutomu Tanaka ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Lactobacillus Plantarum ◽  
Corn Starch ◽  
Optical Purity ◽  
Lactic Acid Fermentation ◽  
Efficient Production ◽  
Acid Fermentation ◽  
Dehydrogenase Gene ◽  
Optically Pure

ABSTRACT In order to achieve direct and efficient fermentation of optically pure d-lactic acid from raw corn starch, we constructed l-lactate dehydrogenase gene (ldhL1)-deficient Lactobacillus plantarum and introduced a plasmid encoding Streptococcus bovis 148 α-amylase (AmyA). The resulting strain produced only d-lactic acid from glucose and successfully expressed amyA. With the aid of secreting AmyA, direct d-lactic acid fermentation from raw corn starch was accomplished. After 48 h of fermentation, 73.2 g/liter of lactic acid was produced with a high yield (0.85 g per g of consumed sugar) and an optical purity of 99.6%. Moreover, a strain replacing the ldhL1 gene with an amyA-secreting expression cassette was constructed. Using this strain, direct d-lactic acid fermentation from raw corn starch was accomplished in the absence of selective pressure by antibiotics. This is the first report of direct d-lactic acid fermentation from raw starch.

scholarly journals Perdeuteration of cholesterol for neutron scattering applications using recombinant Pichia pastoris

2018 ◽  
Vol 212 ◽  
pp. 80-87 ◽  
Author(s):  
Martine Moulin ◽  
Gernot A. Strohmeier ◽  
Melanie Hirz ◽  
Katherine C. Thompson ◽  
Adrian R. Rennie ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Neutron Scattering ◽  
Recombinant Pichia Pastoris

Constitutive expression of alkaline β-mannanase in recombinant Pichia pastoris

2014 ◽  
Vol 49 (12) ◽  
pp. 2025-2029 ◽  
Author(s):  
Taicheng Zhu ◽  
Hongbing Sun ◽  
Pengfei Li ◽  
Yanfen Xue ◽  
Yin Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Constitutive Expression ◽  
Recombinant Pichia Pastoris
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