Transgenic manipulation of plant embryo sacs tracked through cell-type-specific fluorescent markers: cell labeling, cell ablation, and adventitious embryos

2013 ◽  
Vol 26 (2) ◽  
pp. 125-137 ◽  
Author(s):  
Shai J. Lawit ◽  
Mark A. Chamberlin ◽  
April Agee ◽  
Eric S. Caswell ◽  
Marc C. Albertsen
Keyword(s):  
Cell Labeling ◽  
Cell Type ◽  
Fluorescent Markers ◽  
Cell Ablation ◽  
Cell Type Specific ◽  
Plant Embryo ◽  
Transgenic Manipulation

scholarly journals Machine learning sequence prioritization for cell type-specific enhancer design

2021 ◽  
Author(s):  
Alyssa J Lawler ◽  
Easwaran Ramamurthy ◽  
Ashley R Brown ◽  
Naomi Shin ◽  
Yeonju Kim ◽  
...  
Keyword(s):  
Machine Learning ◽  
Affinity Purification ◽  
Rapid Development ◽  
Cell Labeling ◽  
Specific Gene ◽  
Open Chromatin ◽  
Specific Cell ◽  
Cell Type ◽  
Cell Type Specific ◽  
Nuclear Isolation

AbstractRecent discoveries of extreme cellular diversity in the brain warrant rapid development of technologies to access specific cell populations, enabling characterization of their roles in behavior and in disease states. Available approaches for engineering targeted technologies for new neuron subtypes are low-yield, involving intensive transgenic strain or virus screening. Here, we introduce SNAIL (Specific Nuclear-Anchored Independent Labeling), a new virus-based strategy for cell labeling and nuclear isolation from heterogeneous tissue. SNAIL works by leveraging machine learning and other computational approaches to identify DNA sequence features that confer cell type-specific gene activation and using them to make a probe that drives an affinity purification-compatible reporter gene. As a proof of concept, we designed and validated two novel SNAIL probes that target parvalbumin-expressing (PV) neurons. Furthermore, we show that nuclear isolation using SNAIL in wild type mice is sufficient to capture characteristic open chromatin features of PV neurons in the cortex, striatum, and external globus pallidus. Expansion of this technology has broad applications in cell type-specific observation, manipulation, and therapeutics across species and disease models.

IFIH1-deficiency results in a cell-type specific alteration of autophagic activity in response to S. typhimurium

2017 ◽  
Vol 55 (05) ◽  
pp. e28-e56
Author(s):  
S Macheiner ◽  
R Gerner ◽  
A Pfister ◽  
A Moschen ◽  
H Tilg
Keyword(s):  
Cell Type ◽  
A Cell ◽  
Cell Type Specific ◽  
Autophagic Activity ◽  
Specific Alteration

Cell Type–Specific Transcriptional and Epigenetic Profiles from a Rare Neuronal Population within the Arcuate Nucleus

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 2430-PUB
Author(s):  
FRANKIE D. HEYWARD ◽  
EVAN ROSEN
Keyword(s):  
Arcuate Nucleus ◽  
Neuronal Population ◽  
Cell Type ◽  
Cell Type Specific
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