Serum protein electrophoretic pattern in clinically healthy calves and cows determined by agarose gel electrophoresis

Screening of electrophoretic pattern of serum protein is crucial for the initial evaluation of numerous clinical conditions. The present study aimed to investigate and compare the normal serum protein electrophoretic pattern in three gazelle species which included sand gazelle (Gazella gazella), mountain gazelle (Gazella subgutterosa) and Erlanger’s gazelle (Gazella erlangeri). Significant (P £ 0.05) interspecies differences were noticed in A/G ratio, and in á-1, â and globulins fractions using agarose gel electrophoresis. Significant (P £ 0.05) differences between males of the three species were encountered in á-1, á- 2 and â globulins fractions, while significant differences in A/G ratio, á-1, á-2 and â globulins fractions were observed between females of the three species. Significance (P £ 0.05) intersex differences were observed for albumin in sand gazelle, and for á-2 and â globulins fractions in mountain gazelle. The results obtained from the current study could provide a baseline references and could be used for diagnostic purposes.

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Absence of beta-globulin band in the serum protein electropherogram of a patient with liver disease.

Clinical Chemistry ◽

10.1093/clinchem/27.2.334 ◽

1981 ◽

Vol 27 (2) ◽

pp. 334-336 ◽

Cited By ~ 2

Author(s):

A O Vladutiu ◽

J S Kim

Keyword(s):

Liver Cirrhosis ◽

Liver Disease ◽

Serum Protein ◽

Gel Electrophoresis ◽

Marked Decrease ◽

Agarose Gel ◽

Agarose Gel Electrophoresis ◽

Glycoprotein I ◽

Complement Component C3 ◽

Beta Globulin

Abstract Agarose-gel electrophoresis of serum of a 72-year-old woman with liver cirrhosis showed virtually no beta-globulins two weeks before the patient's death. There was marked decrease in the concentrations of transferrin, beta-lipoproteins, hemopexin, complement component C3, beta-glycoprotein I, and cholesterol in serum. Absence of a beta-globulin band appears to signify an ominous prognosis.

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Evaluation of usefulness of a commercial agarose gel electrophoresis kit (QuickGel SP) for bovine serum protein electrophoresis

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2017-0064 ◽

2017 ◽

Vol 20 (3) ◽

pp. 527-534

Author(s):

Y. Okatsu ◽

N. Yamagishi ◽

K. Hatate ◽

B. Devkota

Keyword(s):

Serum Protein ◽

Gel Electrophoresis ◽

Bovine Serum ◽

Protein Fractions ◽

Agarose Gel ◽

Agarose Gel Electrophoresis ◽

Serum Samples ◽

Cellulose Acetate Electrophoresis ◽

Γ Globulins ◽

Bovine Serum Protein

Abstract The aim of this study was to show the usefulness of a commercial agarose gel electrophoresis (AGE) kit (QuickGel SP) for separating bovine serum protein fractions in comparison with conventional cellulose acetate electrophoresis (CAE). Serum protein bands were verified using five reference reagents corresponding to albumin and α1-, β1-, β2-, and γ-globulins. AGE clearly revealed six separated fractions of albumin and α1-, α2-, β1-, β2-, and γ-globulin fractions in 100% and 77.8% in serum samples of dairy cows from the healthy (n=27) and diseased groups (n=27), respectively. The α1- and α2-globulins were not separated by CAE in 14.8% and 96.3% of the samples from the healthy and diseased groups, respectively, whereas β2- and γ-globulin were not separated by CAE in 96.3% and 100% of the samples from the healthy and diseased groups, respectively. More than 94% of the points for the α-globulin fractions (α1- and α2-globulins), the β-γ-globulin fractions (β1-, β2-, and γ-globulins), and the albumin/globulin ratio between AGE and CAE were within agreement on the Bland-Altman plots. However, the mean biases were not near zero in the albumin and β-γ-globulin fractions. These results suggest that the high-resolution commercial AGE kit can be utilized to separate bovine serum protein fractions.

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