Genetic variability in the coat protein genes of two orchid viruses: Cymbidium mosaic virus and Odontoglossum ringspot virus

2002 ◽  
Vol 147 (10) ◽  
pp. 1943-1954 ◽  
Author(s):  
P. A. Ajjikuttira ◽  
C. L. Lim-Ho ◽  
M. H. Woon ◽  
K. H. Ryu ◽  
C. A. Chang ◽  
...  
Keyword(s):  
Coat Protein ◽  
Genetic Variability ◽  
Mosaic Virus ◽  
Ringspot Virus ◽  
Cymbidium Mosaic Virus ◽  
Odontoglossum Ringspot Virus

scholarly journals Reciprocal function of movement proteins and complementation of long-distance movement of Cymbidium mosaic virus RNA by Odontoglossum ringspot virus coat protein

2005 ◽  
Vol 86 (5) ◽  
pp. 1543-1553 ◽  
Author(s):  
Prabha Ajjikuttira ◽  
Chiang-Shiong Loh ◽  
Sek-Man Wong
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Mosaic Virus ◽  
Cell Movement ◽  
Ringspot Virus ◽  
Deficient Mutant ◽  
Long Distance ◽  
Cymbidium Mosaic Virus ◽  
Odontoglossum Ringspot Virus ◽  
Long Distance Movement

Complementation of movement and coat proteins of the orchid-infecting potexvirus Cymbidium mosaic virus (CymMV) and tobamovirus Odontoglossum ringspot virus (ORSV) was investigated. Nicotiana benthamiana, which is susceptible to both CymMV and ORSV, was used as a model system. Four transgenic lines, each harbouring one of the movement protein (MP) or coat protein (CP) genes of CymMV or ORSV, were constructed. The MP of CymMV consists of three overlapping open reading frames, together called the triple-gene block (TGB). CymMV and ORSV mutants, each carrying an inactivated MP or CP, were generated from the respective biologically active full-length cDNA clones. Complementation was studied by infecting transgenic plants with in vitro transcripts generated from these mutants. The cell-to-cell movement of a movement-deficient CymMV was restored in transgenic plants carrying the ORSV MP transgene. Similarly, CymMV TGB1 transgenic plants were able to rescue the cell-to-cell movement of a movement-deficient ORSV mutant. ORSV CP transgenic plants supported systemic movement of a CymMV CP-deficient mutant. However, in these plants, neither encapsidation of CymMV RNA with ORSV CP nor CymMV CP expression was detected. Long-distance movement of an ORSV CP-deficient mutant was not supported by CymMV CP. The complementation of MPs and CPs of CymMV and ORSV facilitates movement of these viruses in plants, except for long-distance movement of ORSV RNA by CymMV CP.

scholarly journals First Report of Cymbidium mosaic virus and Odontoglossum ringspot virus in Orchids in Mexico

Plant Disease ◽  
2012 ◽  
Vol 96 (3) ◽  
pp. 464-464
Author(s):  
A. G. Soto-Valladares ◽  
R. De La Torre-Almaraz ◽  
B. Xoconostle-Cazares ◽  
R. Ruíz-Medrano
Keyword(s):  
Mosaic Virus ◽  
Pcr Amplification ◽  
Positive Sample ◽  
Ringspot Virus ◽  
Rdrp Gene ◽  
Mixed Infections ◽  
Cymbidium Mosaic Virus ◽  
First Report ◽  
Odontoglossum Ringspot Virus ◽  
Cp Gene

In 2010, a survey for viral diseases in commercial, orchid-producing greenhouses was carried out in Morelos, Mexico. Many symptomatic plants were observed. The most common leaf symptoms were yellow mottle, yellow streaks, and chlorotic and necrotic ringspots. Leaf samples were collected from eight symptomatic plants from the following genera: Encyclia, Oncidium, Shomburghia, Brassia, Guarianthe, Cattleya, Epidendrum, Vanilla, Xilobium, Laelia, and Brassocattleya. Samples were tested using double-antibody sandwich (DAS)-ELISA (Agdia, Elkhart, IN) with antiserum for Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), Cymbidium ringspot mosaic virus, and Tobacco mosaic virus (TMV) and a general antiserum for potyviruses. At least one plant from each genus was positive to CymMV and ORSV as individual or mixed infections. Encyclia and Laelia plants were the most frequently found with mixed infections by both viruses. All genera were negative for TMV and potyviruses. Total RNA extracts were obtained from all ELISA-positive samples by a modified silica capture protocol (2). Reverse transcription (RT)-PCR was carried out with general polymerase (RdRp) gene primers corresponding to the Potexvirus group (3) and specific primers for the coat protein gene (CP) of CymMV and ORSV (1). The PCR amplification from a positive sample of each genus was resolved in agarose gels. Amplification products of the expected size were obtained for CymMV and ORSV. Five CymMV RdRp gene clones from five different plants of Laelia (GenBank Accession Nos. HQ393958, HQ393959, HQ393960, HQ393961, and HQ393962), two CP gene clones of CP gene of CymMV from two different plants of Oncidium (GenBank Accession Nos. HQ393956 and HQ393957), and three CP clones of CP of ORSV from three different plants of Encyclia (GenBank Accession Nos. HQ393953, HQ393954, and HQ393955) were sequenced. The nucleotide sequences of the Mexican orchid CymMV isolates were 96 to 97% identical to CymMV sequences in the GenBank, while those of ORSV were 99 to 100% identical to deposited ORSV sequences. To our knowledge, this is the first report of CymMV and ORSV in orchids in Mexico, which are two of the most important quarantine virus in orchids in Mexico. References: (1) P. Ajjikuttira et al. J. Gen. Virol. 86:1543, 2005. (2) J. R. Thompson et al. J. Virol. Methods 111:85, 2003. (3) R. A. A. van der Vlugt and M. Berendsen. Eur. J. Plant Pathol. 108:367, 2002.

scholarly journals Dual resistance of transgenic plants against Cymbidium mosaic virus and Odontoglossum ringspot virus

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ting-Yu Chen ◽  
Hsuan Pai ◽  
Liang-Yu Hou ◽  
Shu-Chuan Lee ◽  
Tzu-Tung Lin ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Ringspot Virus ◽  
Cymbidium Mosaic Virus ◽  
Odontoglossum Ringspot Virus

A two-in-one immunoassay biosensor for the simultaneous detection of Odontoglossum ringspot virus and Cymbidium mosaic virus

2022 ◽  
Vol 350 ◽  
pp. 130875
Author(s):  
Wei-Ping Lin ◽  
Wei-Jhen Wang ◽  
Chia-Hwa Lee ◽  
Fuh-Jyh Jan ◽  
Gou-Jen Wang
Keyword(s):  
Mosaic Virus ◽  
Simultaneous Detection ◽  
Ringspot Virus ◽  
Cymbidium Mosaic Virus ◽  
Odontoglossum Ringspot Virus
Sign in / Sign up

Export Citation Format

Share Document