Genetic analysis of the pX region of bovine leukemia virus genotype 1 in Holstein Friesian cattle with different stages of infection

2021 ◽  
Author(s):  
Neli Montero Machuca ◽  
Jorge Luis Tórtora Pérez ◽  
Ana Silvia González Méndez ◽  
Angélica Lucia García-Camacho ◽  
Ernesto Marín Flamand ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Holstein Friesian ◽  
Friesian Cattle

scholarly journals Genetic Analysis of the pX Region in Bovine Leukemia Virus genotype 1 in Holstein Friesian Cattle with Different Stages of Infection

2021 ◽  
Author(s):  
Neli Montero Machuca ◽  
Jorge Luis Tórtora Pérez ◽  
Ana Silvia González Méndez ◽  
Lucia Angelica García-Camacho ◽  
Ernesto Marín Flamand ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Proviral Load ◽  
Leukemia Virus ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Blood Leukocytes ◽  
Nucleotide Deletion ◽  
Holstein Friesian ◽  
Infected Cells ◽  
Friesian Cattle

Abstract The pX genetic region of the Bovine Leukemia Virus (BLV) includes four genes with overlapping reading frames that code for the Tax, Rex, R3 and G4 proteins. These proteins are involved in the regulation of transcriptional and post-transcriptional viral expression, as well as having oncogenic potential. Our goal was to determine the pathogenic role associated with BLV genotype 1 pX region genetics in terms of lymphocytosis, lymphomas and proviral load. We screened 724 serological samples from mixed-age Holstein Friesian cattle from six states in Mexico. Once peripheral blood leukocytes were isolated from whole blood with anticoagulant, we extracted genomic DNA using a commercial kit. Then, we designed in silico primers that hybridize in conserved regions of the BLV pX region, which allowed for PCR standardization to detect proviral DNA in infected cells. Positive amplicons were sequenced using the Sanger method, obtaining 1156 nucleotide-long final sequences that included the four pX region genes. The 30 heads of cattle that formed the genetic study population included 12 with lymphocytosis, 12 without lymphocytosis and six with lymphoma. Lymphoma presence was determined in six bovine tumor tissues using histopathology, and we identified BLV presence with in situ hybridization.Phylogenetic analysis determined that the 30 sequences were associated with genotype 1, and genetic distance between the sequences ranged from 0.2% - 2.09%. We identified two sequences in the G4 gene, one with a three-nucleotide deletion (AGU_7488L, in a cow with lymphocytosis), and one with a nine nucleotide deletion (AGU_18A, in a cow without lymphocytosis). PX region analysis identified positive selection in the G4, rex and R3 genes, and we found no difference in proviral load between the studied groups. It was not possible to establish an association between pX region variability and the development of lymphocytosis, lymphoma, asymptomatic status and proviral load in BLV-infected cattle.

Association between BoLA‐DRB3 polymorphism and bovine leukemia virus proviral load in Vietnamese Holstein Friesian cattle

HLA ◽  
2021 ◽  
Author(s):  
Dung Le Thi ◽  
Son Nguyen Vu ◽  
Chieh‐Wen Lo ◽  
Tung Duy Dao ◽  
Vuong Nghia Bui ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Proviral Load ◽  
Leukemia Virus ◽  
Holstein Friesian ◽  
Friesian Cattle

Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia

2019 ◽  
Vol 164 (10) ◽  
pp. 2531-2536 ◽  
Author(s):  
Mundia M. Phiri ◽  
Evans Kaimoyo ◽  
Katendi Changula ◽  
Isaac Silwamba ◽  
Herman M. Chambaro ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Bovine Leukemia Virus ◽  
Molecular Detection ◽  
Leukemia Virus ◽  
Genotype 1 ◽  
Traditional Sector

Detection of genotype 1 bovine leukemia virus from a C.schultzei pool: Do Culicoides spp. have a role on the transmission of bovine leukemia virus?

2020 ◽  
Vol 85 ◽  
pp. 104469
Author(s):  
Fırat Dogan ◽  
Seval Bilge Dagalp ◽  
Bilal Dik ◽  
Touraj Aligholipour Farzani ◽  
Feray Alkan
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Genotype 1

scholarly journals MASKED GENOTYPE 6 OF BOVINE LEUKEMIA VIRUS IS FOUND IN COLOMBIAN CATTLE

2019 ◽  
Author(s):  
Adriana Patricia Corredor-Figueroa ◽  
Nury Nathalia Olaya-Galán ◽  
Sandra Patricia Salas ◽  
Juan Sebastián Quintero ◽  
Álvaro Fajardo ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Bovine Leukemia Virus ◽  
Phylogenetic Analyses ◽  
Leukemia Virus ◽  
Gene Analysis ◽  
Economic Losses ◽  
Envelope Gene ◽  
Genotype 1 ◽  
Global Epidemiology ◽  
Bovine Leukosis

Abstract Background Viral diseases such as bovine leukosis are a major cause of health problems associated with economic losses in the livestock industry worldwide. The prevalence of bovine leukosis ranges between 4% and 90%, and this disease is considered endemic. The etiological agent is bovine leukemia virus (BLV). Ten BLV genotypes have been reported based on analysis of complete or partial envelope gene sequences, of which genotype 1 is the most prevalent. However, the genetic variability of BLV variants circulating in Colombia remains unexplored. Therefore, in the present study, we analyzed blood samples collected from 289 cows distributed in 75 farms across the country. PCR amplification of env, gag and tax gene segments was performed. The obtained amplicons were sequenced and then subjected to phylogenetic analyses. Results A total of 62% of the cows present at 92% of the farms were BLV-positive. Genotype 1 was exclusively detected by env and gag gene segments when analyzed using previously reported primers. However, tax gene analysis revealed circulation of genotype 6 variants, which were also detected based on env gene analysis with newly designed primers. These results indicate that current genotyping approaches based on partial env sequencing may bias BLV genetic variability approaches and underestimate the diversity of the detected BLV genotypes. Conclusions This report is the first molecular and epidemiological study of BLV conducted in Colombia and contributes to the global epidemiology of the virus; it also reinforces the great impact of BLV on the country’s livestock and thus is a useful resource for farmers and government entities.

Genetic Analysis for Milk Traits in Different Herds of Holstein Friesian Cattle in Turkey

2001 ◽  
Vol 1 (8) ◽  
pp. 737-741 ◽  
Author(s):  
Hulya Atil ◽  
A. Salah Khattab . ◽  
Cigdem Yakupoglu .
Keyword(s):  
Genetic Analysis ◽  
Milk Traits ◽  
Holstein Friesian ◽  
Friesian Cattle

Association between BoLA and subclinical bovine leukemia virus infection in a herd of Holstein-Friesian cows

1988 ◽  
Vol 27 (5) ◽  
pp. 338-344 ◽  
Author(s):  
Harris A. Lewin ◽  
Ming-Che Wu ◽  
Julie A. Stewart ◽  
Timothy J. Nolan
Keyword(s):  
Virus Infection ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Bovine Leukemia Virus Infection ◽  
Holstein Friesian ◽  
Friesian Cows

scholarly journals Prevalence of Lymphocyte Nuclear Pockets in Holstein-Friesian Dairy Cattle Infected with Bovine Leukemia Virus in Korea

2005 ◽  
Vol 18 (6) ◽  
pp. 879-883 ◽  
Author(s):  
S. S. Yoon ◽  
J. W. Park ◽  
Y. H. Jean ◽  
H. J. Kim ◽  
B. Han ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Holstein Friesian
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