The coat protein gene of grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants

1997 ◽  
Vol 142 (6) ◽  
pp. 1101-1116 ◽  
Author(s):  
K. -S. Ling ◽  
H. -Y. Zhu ◽  
H. Alvizo ◽  
J. S. Hu ◽  
R. F. Drong ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Nucleotide Sequencing ◽  
Sequencing And Expression

scholarly journals Restoration of Wild-Type Virus by Double Recombination of Tombusvirus Mutants with a Host Transgene

1999 ◽  
Vol 12 (2) ◽  
pp. 153-162 ◽  
Author(s):  
Marise Borja ◽  
Teresa Rubio ◽  
Herman B. Scholthof ◽  
Andrew O. Jackson
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Wild Type Virus ◽  
Type Virus ◽  
Virus Infections ◽  
Wild Type ◽  
Transgenic Lines ◽  
Double Recombination

Nicotiana benthamiana plants transformed with the coat protein gene of tomato bushy stunt virus (TBSV) failed to elicit effective virus resistance when inoculated with wild-type virus. Subsequently, R1 and R2 progeny from 13 transgenic lines were inoculated with a TBSV mutant containing a defective coat protein gene. Mild symptoms typical of those elicited in nontransformed plants infected with the TBSV mutant initially appeared. However, within 2 to 4 weeks, up to 20% of the transgenic plants sporadically began to develop the lethal syndrome characteristic of wild-type virus infections. RNA hybridization and immunoblot analyses of these plants and nontransformed N. benthamiana inoculated with virus from the transgenic lines indicated that wild-type virus had been regenerated by a double recombination event between the defective virus and the coat protein transgene. Similar results were obtained with a TBSV deletion mutant containing a nucleotide sequence marker, and with a chimeric cucumber necrosis virus (CNV) containing the defective TBSV coat protein gene. In both cases, purified virions contained wild-type TBSV RNA or CNV chimeric RNA derived by recombination with the transgenic coat protein mRNA. These results thus demonstrate that recombinant tombusviruses can arise frequently from viral genes expressed in transgenic plants.

IDENTIFICATION OF THE COAT PROTEIN GENE OF ARABIS MOSAIC NEPOVIRUS AND ITS EXPRESSION IN TRANSGENIC PLANTS

1992 ◽  
pp. 37-42 ◽  
Author(s):  
H. Steinkellner ◽  
A. Weinhäusl ◽  
M. Laimer ◽  
A. Machado ◽  
H. Katinger
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene

Analysis of transitive RNA silencing after grafting in transgenic plants with the coat protein gene of Sweet potato feathery mottle virus

2006 ◽  
Vol 63 (1) ◽  
pp. 35-47 ◽  
Author(s):  
A. K. M. N. Haque ◽  
Yoshikazu Tanaka ◽  
Shoji Sonoda ◽  
Masamichi Nishiguchi
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Sweet Potato ◽  
Coat Protein Gene ◽  
Rna Silencing ◽  
Protein Gene ◽  
Mottle Virus

Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus

1992 ◽  
Vol 11 (1) ◽  
pp. 25-29 ◽  
Author(s):  
Margit Laimer da Câmara Machado ◽  
Artur da Câmara Machado ◽  
Veronika Hanzer ◽  
Hans Weiss ◽  
Ferdinand Regner ◽  
...  
Keyword(s):  
Coat Protein ◽  
Transgenic Plants ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Prunus Armeniaca ◽  
Plum Pox Virus
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