Site-Specific Detection of Free Radicals in Membranes Using an Amphiphilic Spin Trap

2015 ◽  
Vol 46 (5) ◽  
pp. 489-504 ◽  
Author(s):  
Saipeng Huang ◽  
Zhao Chen ◽  
Libo Du ◽  
Qiu Tian ◽  
Yangping Liu ◽  
...  
Keyword(s):  
Free Radicals ◽  
Spin Trap ◽  
Specific Detection ◽  
Site Specific

scholarly journals Estimation of the Postmortem Duration of Mouse Tissue by Electron Spin Resonance Spectroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-11
Author(s):  
Shinobu Ito ◽  
Tomohisa Mori ◽  
Hideko Kanazawa ◽  
Toshiko Sawaguchi
Keyword(s):  
Free Radicals ◽  
Electron Spin Resonance ◽  
Electron Spin ◽  
Spin Trap ◽  
Detection Sensitivity ◽  
Mouse Tissue ◽  
Spin Adduct ◽  
Simple Method ◽  
Oxidation Stress ◽  
Spin Resonance

Electron spin resonance (ESR) method is a simple method for detecting various free radicals simultaneously and directly. However, ESR spin trap method is unsuited to analyze weak ESR signals in organs because of water-induced dielectric loss (WIDL). To minimize WIDL occurring in biotissues and to improve detection sensitivity to free radicals in tissues, ESR cuvette was modified and used with 5,5-dimethtyl-1-pyrroline N-oxide (DMPO). The tissue samples were mouse brain, hart, lung, liver, kidney, pancreas, muscle, skin, and whole blood, where various ESR spin adduct signals including DMPO-ascorbyl radical (AsA∗), DMPO-superoxide anion radical (OOH), and DMPO-hydrogen radical (H) signal were detected. Postmortem changes in DMPO-AsA∗and DMPO-OOH were observed in various tissues of mouse. The signal peak of spin adduct was monitored until the 205th day postmortem. DMPO-AsA∗in liver (y=113.8–40.7 log (day),R1=-0.779,R2=0.6,P<.001) was found to linearly decrease with the logarithm of postmortem duration days. Therefore, DMPO-AsA∗signal may be suitable for detecting an oxidation stress tracer from tissue in comparison with other spin adduct signal on ESR spin trap method.

Site-Specific Detection of Arginine Methylation in Highly Repetitive Protein Motifs of Low Sequence Complexity by NMR

2020 ◽  
Vol 142 (16) ◽  
pp. 7647-7654
Author(s):  
Nadide Altincekic ◽  
Frank Löhr ◽  
Jakob Meier-Credo ◽  
Julian D. Langer ◽  
Martin Hengesbach ◽  
...  
Keyword(s):  
Arginine Methylation ◽  
Specific Detection ◽  
Site Specific ◽  
Protein Motifs ◽  
Sequence Complexity ◽  
Repetitive Protein

Site-Specific Detection of Bleeder Using Transesophageal Echocardiography

2005 ◽  
Vol 13 (4) ◽  
pp. 366-368
Author(s):  
Harpreet Wasir ◽  
Sanjay Mittal ◽  
Yugal Mishra ◽  
Yatin Mehta ◽  
Naresh Trehan
Keyword(s):  
Cardiac Output ◽  
Transesophageal Echocardiography ◽  
Bypass Surgery ◽  
Surgical Intervention ◽  
Coronary Bypass ◽  
Surgical Patients ◽  
Operating Table ◽  
Specific Detection ◽  
Site Specific ◽  
Low Cardiac Output

Transesophageal echocardiography continues to be an indispensable postoperative diagnostic tool for cardiac surgical patients. Transesophageal echocardiography was carried out postoperatively in 30 consecutive hypotensive patients with low cardiac output who had undergone coronary bypass surgery. In 19 of these patients, a cause of low cardiac output requiring surgical intervention was excluded, and they were managed conservatively. In 11 patients, a surgical cause of low cardiac output was indicated: diffuse bleeding from no particular site in 5, and from a specific site in 6. They underwent urgent re-operation, and the echocardiography findings were confirmed on the operating table. Not only is transesophageal echocardiography important in diagnosis, but it is also highly specific in locating the site of bleeding.

scholarly journals Single-molecule site-specific detection of protein phosphorylation with a nanopore

2014 ◽  
Vol 32 (2) ◽  
pp. 179-181 ◽  
Author(s):  
Christian B Rosen ◽  
David Rodriguez-Larrea ◽  
Hagan Bayley
Keyword(s):  
Protein Phosphorylation ◽  
Single Molecule ◽  
Specific Detection ◽  
Site Specific
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