In vitro culture of human dental pulp cells: some aspects of cells emerging early from the explant

1997 ◽  
Vol 1 (3) ◽  
pp. 131-140 ◽  
Author(s):  
L. Stanislawski ◽  
J. P. Carreau ◽  
M. Pouchelet ◽  
Z. H. J. Chen ◽  
M. Goldberg
Keyword(s):  
In Vitro Culture ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In vitro characterization of human dental pulp cells: various isolation methods and culturing environments

2006 ◽  
Vol 324 (2) ◽  
pp. 225-236 ◽  
Author(s):  
George T.-J. Huang ◽  
Wataru Sonoyama ◽  
James Chen ◽  
Sang Hyuk Park
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
In Vitro Characterization ◽  
Isolation Methods ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Se-Min Kim ◽  
Bin-Na Lee ◽  
Jeong-Tae Koh ◽  
Hoon-Sang Chang ◽  
In-Nam Hwang ◽  
...  
Keyword(s):  
Dental Pulp ◽  
In Vitro Study ◽  
Vitro Study ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Chlormadinone Acetate ◽  
Odontogenic Differentiation ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals Highly Proliferative Immortalized Human Dental Pulp Cells Retain the Odontogenic Phenotype when Combined with a Beta-Tricalcium Phosphate Scaffold and BMP2

2020 ◽  
Vol 2020 ◽  
pp. 1-18 ◽  
Author(s):  
Xiangfen Li ◽  
Liu Wang ◽  
Qin Su ◽  
Ling Ye ◽  
Xuedong Zhou ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Dental Pulp ◽  
Biological Behavior ◽  
Dental Pulp Cells ◽  
Differentiation Potential ◽  
Human Dental Pulp Cells ◽  
Beta Tricalcium Phosphate ◽  
Human Dental Pulp ◽  
Pulp Cells

Human dental pulp cells (HDPCs) play a vital role in dentin formation and reparative dentinogenesis, which indicated their potential application in regenerative medicine. However, HDPCs, which can only be obtained from scarce human pulp tissues, also have a limited lifespan in vitro, and stem cells usually lose their original characteristics over a large number of passages. To overcome these challenges, we successfully immortalized human dental pulp cells using the piggyBac system which was employed to efficiently overexpress the SV40 T-Ag, and we then comprehensively described the cell biological behavior. The immortalized human dental pulp cells (iHDPCs) acquired long-term proliferative activity and expressed most HDPC markers. The iHDPCs maintained multiple differentiation potential and could be induced to differentiate into chondrogenic, osteogenic, and adipogenic cells in vitro. We also proved that the iHDPCs gained a stronger ability to migrate than the primary cells, while apoptosis was inhibited. Furthermore, highly proliferative iHDPCs displayed no oncogenicity when subcutaneously implanted into athymic nude mice. Finally, iHDPCs exhibited odontogenic differentiation ability and secreted dentin sialophosphoprotein (DSPP) when combined with a beta-tricalcium phosphate scaffold and bone morphogenetic protein-2 (BMP2) in vivo. Conclusively, the established iHDPCs are a valuable resource for mechanistic study of dental pulp cell differentiation and dental pulp injury repair, as well as for applications in tooth regeneration.

Evaluation of Cannabinoids on the Odonto/Osteogenesis in Human Dental Pulp Cells In Vitro

2021 ◽  
Vol 47 (3) ◽  
pp. 444-450
Author(s):  
Xia Qi ◽  
Chunyan Liu ◽  
Guohua Li ◽  
Dalia Al-Alfe ◽  
Susan Paurazas ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In Vitro Analysis of Scaffold-free Prevascularized Microtissue Spheroids Containing Human Dental Pulp Cells and Endothelial Cells

2015 ◽  
Vol 41 (5) ◽  
pp. 663-670 ◽  
Author(s):  
Waruna Lakmal Dissanayaka ◽  
Lifang Zhu ◽  
Kenneth M. Hargreaves ◽  
Lijian Jin ◽  
Chengfei Zhang
Keyword(s):  
Endothelial Cells ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Vitro Analysis ◽  
In Vitro Analysis
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