Biochemical characterization and structural analysis of a new cold-active and salt-tolerant esterase from the marine bacterium Thalassospira sp.

Extremophiles ◽  
2016 ◽  
Vol 20 (3) ◽  
pp. 323-336 ◽  
Author(s):  
Concetta De Santi ◽  
Hanna-Kirsti S. Leiros ◽  
Alessia Di Scala ◽  
Donatella de Pascale ◽  
Bjørn Altermark ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Marine Bacterium ◽  
Biochemical Characterization ◽  
Salt Tolerant ◽  
Cold Active

A New Alkaliphilic Cold-Active Esterase from the Psychrophilic Marine Bacterium Rhodococcus sp.: Functional and Structural Studies and Biotechnological Potential

2014 ◽  
Vol 172 (6) ◽  
pp. 3054-3068 ◽  
Author(s):  
Concetta De Santi ◽  
Pietro Tedesco ◽  
Luca Ambrosino ◽  
Bjørn Altermark ◽  
Nils-Peder Willassen ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Structural Studies ◽  
Biotechnological Potential ◽  
Cold Active ◽  
Rhodococcus Sp

Separation, biochemical characterization and salt‐tolerant mechanisms of alkaline protease from Aspergillus oryzae

2019 ◽  
Vol 99 (7) ◽  
pp. 3359-3366 ◽  
Author(s):  
Xianli Gao ◽  
Yiyun Yin ◽  
Jingkun Yan ◽  
Junke Zhang ◽  
Haile Ma ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Alkaline Protease ◽  
Biochemical Characterization ◽  
Salt Tolerant

A Novel Cold-Active and Alkali-Stable β-Glucosidase Gene Isolated from the Marine Bacterium Martelella mediterranea

2010 ◽  
Vol 162 (8) ◽  
pp. 2136-2148 ◽  
Author(s):  
Xiaoxia Mao ◽  
Yuzhi Hong ◽  
Zongze Shao ◽  
Yan Zhao ◽  
Ziduo Liu
Keyword(s):  
Marine Bacterium ◽  
Cold Active

Overexpression and characterization of a novel cold-adapted and salt-tolerant GH1 β-glucosidase from the marine bacterium Alteromonas sp. L82

2018 ◽  
Vol 56 (9) ◽  
pp. 656-664 ◽  
Author(s):  
Jingjing Sun ◽  
Wei Wang ◽  
Congyu Yao ◽  
Fangqun Dai ◽  
Xiangjie Zhu ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Salt Tolerant ◽  
Cold Adapted

Cloning, purification and biochemical characterization of beta agarase from the marine bacterium Pseudoalteromonas sp. AG4

2010 ◽  
Vol 37 (5) ◽  
pp. 483-494 ◽  
Author(s):  
Chulhong Oh ◽  
Chamilani Nikapitiya ◽  
Youngdeuk Lee ◽  
Ilson Whang ◽  
Se-Jae Kim ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Biochemical Characterization

scholarly journals Discovery and characterization of a novel family of prokaryotic nanocompartments involved in sulfur metabolism

2020 ◽  
Author(s):  
Robert J. Nichols ◽  
Benjamin LaFrance ◽  
Naiya R. Phillips ◽  
Luke M. Oltrogge ◽  
Luis E. Valentin-Alvarado ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Biochemical Characterization ◽  
Sulfur Metabolism ◽  
Physiological Role ◽  
Enzymatic Characterization ◽  
Cysteine Desulfurase ◽  
Future Studies ◽  
Pcc 7942

AbstractProkaryotic nanocompartments, also known as encapsulins, are a recently discovered proteinaceous organelle in prokaryotes that compartmentalize cargo enzymes. While initial studies have begun to elucidate the structure and physiological roles of encapsulins, bioinformatic evidence suggests that a great diversity of encapsulin nanocompartments remains unexplored. Here, we describe a novel encapsulin in the freshwater cyanobacterium Synechococcus elongatus PCC 7942. This nanocompartment is upregulated upon sulfate starvation and encapsulates a cysteine desulfurase enzyme via an N-terminal targeting sequence. Using cryoelectron microscopy, we have determined the structure of the nanocompartment complex to 2.2 Å resolution. Lastly, biochemical characterization of the complex demonstrated that the activity of the cysteine desulfurase is enhanced upon encapsulation. Taken together, our discovery, structural analysis, and enzymatic characterization of this prokaryotic nanocompartment provide a foundation for future studies seeking to understand the physiological role of this encapsulin in various bacteria.

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