Prospective evaluation of a real-time PCR assay for detection of group B streptococci in vaginal swabs from pregnant women

2005 ◽  
Vol 24 (5) ◽  
pp. 355-357 ◽  
Author(s):  
H. Réglier-Poupet ◽  
G. Quesne ◽  
E. Théo ◽  
M. Dommergues ◽  
P. Berche ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Prospective Evaluation ◽  
Pcr Assay ◽  
Group B Streptococci ◽  
Vaginal Swabs ◽  
Group B

scholarly journals Evaluation of a novel real-time PCR test based on the ssrAgene for the identification of group B streptococci in vaginal swabs

2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Martina Wernecke ◽  
Ciara Mullen ◽  
Vimla Sharma ◽  
John Morrison ◽  
Thomas Barry ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococci ◽  
Vaginal Swabs ◽  
Group B ◽  
Pcr Test

scholarly journals Development of Conventional and Real-Time PCR Assays for the Rapid Detection of Group B Streptococci

2000 ◽  
Vol 46 (3) ◽  
pp. 324-331 ◽  
Author(s):  
Danbing Ke ◽  
Christian Ménard ◽  
François J Picard ◽  
Maurice Boissinot ◽  
Marc Ouellette ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Culture Method ◽  
Conventional Pcr ◽  
Pcr Assay ◽  
Group B Streptococci ◽  
Standard Culture ◽  
Pcr Assays ◽  
Group B

Abstract Background: Group B streptococci (GBS), or Streptococcus agalactiae, are the leading bacterial cause of meningitis and bacterial sepsis in newborns. Currently available rapid methods to detect GBS from clinical specimens are unsuitable for replacement of culture methods, mainly because of their lack of sensitivity. Methods: We have developed a PCR-based assay for the rapid detection of GBS. The cfb gene encoding the Christie-Atkins-Munch-Petersen (CAMP) factor was selected as the genetic target for the assay. The PCR primers were initially tested by a conventional PCR method followed by gel electrophoresis. The assay was then adapted for use with the LightCyclerTM. For this purpose, two fluorogenic adjacent hybridization probes complementary to the GBS-specific amplicon were designed and tested. In addition, a rapid sample-processing protocol was evaluated by colony-forming unit counting and PCR. A total of 15 vaginal samples were tested by both standard culture method and the two PCR assays. Results: The conventional PCR assay was specific because it amplified only GBS DNA among 125 bacterial and fungal species tested, and was able to detect all 162 GBS isolates from various geographical areas. This PCR assay allowed detection of as few as one genome copy of GBS. The real-time PCR assay was comparable to conventional PCR assay in terms of sensitivity and specificity, but it was more rapid, requiring only ∼30 min for amplification and computer-based data analysis. The presence of vaginal specimens had no detrimental effect on the sensitivity of the PCR with the sample preparation protocol used. All four GBS-positive samples identified by the standard culture method were detected by the two PCR assays. Conclusion: These assays provide promising tools for the rapid detection and identification of GBS.

scholarly journals Application of Real-time PCR to Identify Carriers of Group B Streptococci (GBS) in Pregnant Women

2019 ◽  
Vol 93 (4) ◽  
pp. 507-514
Author(s):  
Tsuyoshi NAKAHARA ◽  
Kiyoko TAMAI ◽  
Miyuki MOROZUMI ◽  
Kimiko UBUKATA
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococci ◽  
Group B

P1422 Development and validation of a real-time PCR assay for the detection of group B Streptococcus in pregnant women

2007 ◽  
Vol 29 ◽  
pp. S396
Author(s):  
M. Wernecke ◽  
C. Mullen ◽  
M. Maher ◽  
T. Barry ◽  
T. Smith
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococcus ◽  
Pcr Assay ◽  
Group B ◽  
Development And Validation

Real-time PCR-assay in the delivery suite for determination of group B streptococcal colonization in a setting with risk-based antibiotic prophylaxis

2013 ◽  
Vol 27 (4) ◽  
pp. 328-332 ◽  
Author(s):  
Stellan Håkansson ◽  
Karin Källén ◽  
Maria Bullarbo ◽  
Per-Åke Holmgren ◽  
Katarina Bremme ◽  
...  
Keyword(s):  
Antibiotic Prophylaxis ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Group B

scholarly journals P2.015 Evaluation of the Triplex Real-Time PCR Assay For Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae in Urine and Vaginal Swabs

2013 ◽  
Vol 89 (Suppl 1) ◽  
pp. A92.1-A92
Author(s):  
T Samleerat ◽  
S Pookkapund ◽  
S Techanun ◽  
K Jitvatcharanon ◽  
P Leechanachai
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Vaginal Swabs

Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood

2004 ◽  
Vol 50 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Stephen M. Golden ◽  
David M. Stamilio ◽  
Brian M. Faux ◽  
Wilfred P. dela Cruz ◽  
Craig T. Shoemaker ◽  
...  
Keyword(s):  
Real Time ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Group B Streptococci ◽  
Fluorescent Pcr ◽  
Neonatal Blood ◽  
Group B

scholarly journals Real-Time PCR Improves Detection ofTrichomonas vaginalisInfection Compared With Culture Using Self-Collected Vaginal swabs

2005 ◽  
Vol 13 (3) ◽  
pp. 145-150 ◽  
Author(s):  
A. M. Caliendo ◽  
J. A. Jordan ◽  
A. M. Green ◽  
J. Ingersoll ◽  
R. J. Diclemente ◽  
...  
Keyword(s):  
African American Women ◽  
Real Time ◽  
Real Time Pcr ◽  
Trichomonas Vaginalis ◽  
Repeated Sequence ◽  
Adolescent And Young Adult ◽  
Broth Culture ◽  
Pcr Assay ◽  
The Real ◽  
Vaginal Swabs

Objective.To compare a real-time polymerase chain reaction (PCR) assay with broth culture for the detection ofTrichomonas vaginalisusing self-collected vaginal swabs.Methods.Self-collected vaginal swabs were obtained from adolescent and young adult African-American women participating in HIV-1 prevention programs.T. vaginalisculture was performed using the InPouch TV System. Samples for the real-time PCR assay were collected using the BDProbeTec ET Culturette Direct Dry Swab system and tested in a laboratory-developed assay which targeted a repeated sequence of the genome. Discrepant samples that were culture negative and positive in the real-time PCR assay were tested in a confirmatory PCR which targeted a different region of theT. vaginalisgenome, the18S ribosomal DNA gene.Results.Of the 524 specimens tested by both culture and real-time PCR, 36 were culture positive and 54 were positive in the real-time PCR assay; 16 of the 18 discrepant specimens were also positive in the confirmatory PCR assay. Using a modified gold standard of positive by culture or positive in both PCR assays, the sensitivity of the real-time PCR assay was 100% and the specificity was 99.6%, whereas culture had a sensitivity of 69.2% and a specificity of 100%.Conclusions.The real-time PCR assay was sensitive and specific for the detection ofT. vaginalisDNA from self-collected vaginal swab specimens. The ability to use the BDProbeTec dry swab system for the real-time PCR testing allowed for the detection ofChlamydia trachomatis, Neisseria gonorrhoeae,andT. vaginalisfrom a single specimen.

scholarly journals Real-Time PCR Assay Provides Reliable Assessment of Intrapartum Carriage of Group B Streptococcus

2010 ◽  
Vol 48 (9) ◽  
pp. 3095-3099 ◽  
Author(s):  
M. J. Alfa ◽  
S. Sepehri ◽  
P. De Gagne ◽  
M. Helawa ◽  
G. Sandhu ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Group B Streptococcus ◽  
Pcr Assay ◽  
Reliable Assessment ◽  
Group B
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