Evaluation of species-specific score cutoff values of routinely isolated clinically relevant bacteria using a direct smear preparation for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based bacterial identification

2011 ◽  
Vol 31 (6) ◽  
pp. 1109-1119 ◽  
Author(s):  
F. Szabados ◽  
H. Tix ◽  
A. Anders ◽  
M. Kaase ◽  
S. G. Gatermann ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Bacterial Identification ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Smear Preparation ◽  
Species Specific ◽  
Clinically Relevant Bacteria ◽  
Matrix Assisted Laser ◽  
Specific Score

scholarly journals Identification of molecularly defined Staphylococcus aureus strains using matrix-assisted laser desorption/ionization time of flight mass spectrometry and the Biotyper 2.0 database

2010 ◽  
Vol 59 (7) ◽  
pp. 787-790 ◽  
Author(s):  
Florian Szabados ◽  
Jaroslaw Woloszyn ◽  
Cindy Richter ◽  
Martin Kaase ◽  
Sören Gatermann
Keyword(s):  
Laser Desorption ◽  
Bacterial Identification ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Identification Approach ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has recently been introduced for bacterial identification. To our knowledge, this is the first study where the Biotyper 2.0 database (Bruker Daltonics) has been applied for bacterial identification in a local strain collection of molecularly defined Staphylococcus aureus. We showed that the accuracy of the Biotyper 2.0-based identification for 602 molecularly defined strains of S. aureus, irrespective of meticillin resistance, was equivalent to that of the molecularly defined reference even at a score cut-off value of 2. Also, 412 isolates of 20 different species of non-S. aureus staphylococci were all correctly identified to species level compared to the molecularly defined reference. Moreover, the MALDI-TOF MS-based S. aureus identification approach was clearly faster than more time-consuming methods such as a molecular identification approach.

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