Cloning and Characterization of Alginate Lyase from a Marine Bacterium Streptomyces sp. ALG-5

2008 ◽  
Vol 11 (1) ◽  
pp. 10-16 ◽  
Author(s):  
Dong Eun Kim ◽  
Eun Yeol Lee ◽  
Hee Sook Kim
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Streptomyces Sp

scholarly journals Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07

Marine Drugs ◽  
2018 ◽  
Vol 16 (3) ◽  
pp. 86 ◽  
Author(s):  
Peng Chen ◽  
Yueming Zhu ◽  
Yan Men ◽  
Yan Zeng ◽  
Yuanxia Sun
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Bacterium Bacillus

scholarly journals Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 308 ◽  
Author(s):  
Yanan Wang ◽  
Xuehong Chen ◽  
Xiaolin Bi ◽  
Yining Ren ◽  
Qi Han ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Specific Activity ◽  
Alginate Lyase ◽  
Initial Activity ◽  
Alginate Oligosaccharides ◽  
Wide Ph Range ◽  
Encoding Gene ◽  
Ph Range ◽  
Alginate Lyases

Alginate oligosaccharides (AOS) show versatile bioactivities. Although various alginate lyases have been characterized, enzymes with special characteristics are still rare. In this study, a polysaccharide lyase family 7 (PL7) alginate lyase-encoding gene, aly08, was cloned from the marine bacterium Vibrio sp. SY01 and expressed in Escherichia coli. The purified alginate lyase Aly08, with a molecular weight of 35 kDa, showed a specific activity of 841 U/mg at its optimal pH (pH 8.35) and temperature (45 °C). Aly08 showed good pH-stability, as it remained more than 80% of its initial activity in a wide pH range (4.0–10.0). Aly08 was also a thermo-tolerant enzyme that recovered 70.8% of its initial activity following heat shock treatment for 5 min. This study also demonstrated that Aly08 is a polyG-preferred enzyme. Furthermore, Aly08 degraded alginates into disaccharides and trisaccharides in an endo-manner. Its thermo-tolerance and pH-stable properties make Aly08 a good candidate for further applications.

scholarly journals Purification and characterization of the extracellular alginate lyase from Streptomyces sp. MET 0515

2007 ◽  
Vol 17 (5) ◽  
pp. 625-633 ◽  
Author(s):  
Hyun-Kyoung Kim ◽  
Jae-Chang Lee ◽  
Nam-Hyun Kang ◽  
Song-Hee Kim ◽  
Jong-Guk Kim ◽  
...  
Keyword(s):  
Alginate Lyase ◽  
Purification And Characterization ◽  
Streptomyces Sp

Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp.

2013 ◽  
Vol 35 (5) ◽  
pp. 703-708 ◽  
Author(s):  
Ya Wang ◽  
En-Wen Guo ◽  
Wen-Gong Yu ◽  
Feng Han
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Purification And Characterization
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