scholarly journals Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07

Marine Drugs ◽  
2018 ◽  
Vol 16 (3) ◽  
pp. 86 ◽  
Author(s):  
Peng Chen ◽  
Yueming Zhu ◽  
Yan Men ◽  
Yan Zeng ◽  
Yuanxia Sun
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Bacterium Bacillus

Purification and characterization of a new alginate lyase from a marine bacterium Vibrio sp.

2013 ◽  
Vol 35 (5) ◽  
pp. 703-708 ◽  
Author(s):  
Ya Wang ◽  
En-Wen Guo ◽  
Wen-Gong Yu ◽  
Feng Han
Keyword(s):  
Marine Bacterium ◽  
Alginate Lyase ◽  
Purification And Characterization

scholarly journals Cloning and Characterization of a Cold-adapted Chitosanase from Marine Bacterium Bacillus sp. BY01

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3915 ◽  
Author(s):  
Yue Yang ◽  
Zhou Zheng ◽  
Yifei Xiao ◽  
Jiaojiao Zhang ◽  
Yu Zhou ◽  
...  
Keyword(s):  
Glycoside Hydrolase ◽  
Marine Bacterium ◽  
Volume Concentration ◽  
Biological Activities ◽  
Maximum Activity ◽  
Bacillus Sp ◽  
Cold Adapted ◽  
Encoding Gene ◽  
Bacterium Bacillus

Chitosanase plays an important role in the production of chitooligosaccharides (CHOS), which possess various biological activities. Herein, a glycoside hydrolase (GH) family 46 chitosanase-encoding gene, csnB, was cloned from marine bacterium Bacillus sp. BY01 and heterologously expressed in Escherichia coli. The recombinant chitosanase, CsnB, was optimally active at 35 °C and pH 5.0. It was also revealed to be a cold-adapted enzyme, maintaining 39.5% and 40.4% of its maximum activity at 0 and 10 °C, respectively. Meanwhile, CsnB showed wide pH-stability within the range of pH 3.0 to 7.0. Then, an improved reaction condition was built to enhance its thermostability with a final glycerol volume concentration of 20%. Moreover, CsnB was determined to be an endo-type chitosanase, yielding chitosan disaccharides and trisaccharides as the main products. Overall, CsnB provides a new choice for enzymatic CHOS production.

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