Viability of white spot syndrome virus (WSSV) in shrimp pond sediments with reference to physicochemical properties

This research study tested the protective effect of prolonged exposure of shrimp food supplemented with glucans from shrimp-pond related yeasts on shrimp Penaeus vannamei reared under the presence of the white spot syndrome virus (WSSV). The glucans extracted and purified from isolated marine yeasts identified as Debaryomyces hansenii, Candida tropicalis, Candida humilis, Candida glabrata, Pichia kudriavzevyi, Wickerhamomyces anomalus and the terrestrial Saccharomyces cerevisiae yeast were characterized by the Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy. The treatments were prepared with food enriched with the yeast beta-glucans and the control groups without beta-glucans. Shrimp were fed thrice a day and challenged orally with WSSV on days 31, 54, 66 and muscularly at day 70. The animals were assessed for the protective effect in terms of post-infection total hemocyte counts, and survival rate. The results indicated that marine yeasts possessed ß-1,3/1,6-glucans, and that D. hansenii was an excellent source yielding 30% of its dry biomass of pure glucans. For the positive control group where no glucans were added, WSSV challenges showed 100% survival when the virus was provided orally, and 40% when the virus was injected. These results also indicated that the shrimp line selected for this study was a resistant line for WSSV. Shrimp groups fed with glucans of the marine yeasts D. hansenii and C. humillis showed a significant protection, allowing shrimp survival of 66% while terrestrial yeast showed 57.14%. These results indicated that marine yeasts growing in the shrimp pond were an excellent source of beta-glucans that allowed extra protection against the mortality caused by this pathogenic virus.

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Tangential flow ultrafiltration for detection of white spot syndrome virus (WSSV) in shrimp pond water

Journal of Virological Methods ◽

10.1016/j.jviromet.2015.03.001 ◽

2015 ◽

Vol 218 ◽

pp. 7-13 ◽

Cited By ~ 1

Author(s):

S.V. Alavandi ◽

R. Ananda Bharathi ◽

S. Satheesh Kumar ◽

N. Dineshkumar ◽

C. Saravanakumar ◽

...

Keyword(s):

White Spot Syndrome Virus ◽

Pond Water ◽

White Spot ◽

Shrimp Pond ◽

Tangential Flow ◽

White Spot Syndrome

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CLUSTER MODEL FOR EXTENSIVE GIANT TIGER SHRIMP (Penaeus monodon Fab.) TO PREVENT TRANSMISSION OF WHITE SPOT SYNDROME VIRUS

Indonesian Aquaculture Journal ◽

10.15578/iaj.10.1.2015.65-70 ◽

2015 ◽

Vol 10 (1) ◽

pp. 65

Author(s):

Arief Taslihan ◽

Richard Callinan ◽

Jenny-Ann Torribio ◽

Bambang Sumiarto ◽

Kamiso Handoyo Nitimulyo

Keyword(s):

Cluster Model ◽

Management Practices ◽

White Spot Syndrome Virus ◽

Penaeus Monodon ◽

White Spot ◽

Total Production ◽

Shrimp Ponds ◽

Shrimp Pond ◽

Tiger Shrimp ◽

White Spot Syndrome

White spot syndrome virus (WSSV) has become epidemic in Indonesia and affecting shrimp aquaculture interm of its production. White spot syndrome virus is transmitted from one to other ponds, through crustacean, included planktonic copepode as carrier for WSSV and through water from affected shrimp pond. A cluster model, consist of shrimp grow out ponds surrounded by non-shrimp pond as a role of biosecurity has been developed. The model aimed to prevent white spot virus transmission in extensive giant tiger shrimp pond. The study was conducted in two sites at Demak District, Central Java Province. As the treatment, a cluster consist of three shrimp ponds in site I, and two shrimp ponds in site II, each was surrounded by buffer ponds rearing only finfish. As the control, five extensive shrimp grow out ponds in site I and three shrimp grow out ponds in site II, with shrimp pond has neither applied biosecurity nor surrounded by non-shrimp pond as biosecurity as well considered as control ponds. The results found that treatment of cluster shrimp ponds surrounded by non-shrimp ponds could hold shrimp at duration of culture in the grow out pond (DOC) 105.6±4.5 days significantly much longer than that of control that harvested at 60.9±16.0 days due to WSSV outbreak. Survival rate in trial ponds was 77.6±3.6%, significantly higher than that of control at 22.6±15.8%. Shrimp production in treatment ponds has total production of 425.1±146.6 kg/ha significantly higher than that of control that could only produced 54.5±47.6 kg/ha. Implementation of Better Management Practices (BMP) by arranging shrimp ponds in cluster and surrounding by non-shrimp ponds proven effectively prevent WSSV transmission from traditional shrimp ponds in surrounding area.

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