Designing a whole-cell biotransformation system in Escherichia coli using cytochrome P450 from Streptomyces peucetius

2008 ◽  
Vol 30 (6) ◽  
pp. 1101-1106 ◽  
Author(s):  
Pramod Shrestha ◽  
Tae-Jin Oh ◽  
Jae Kyung Sohng
Keyword(s):  
Escherichia Coli ◽  
Cytochrome P450 ◽  
Whole Cell ◽  
Streptomyces Peucetius ◽  
Whole Cell Biotransformation ◽  
Biotransformation System

scholarly journals Characterization of P450 FcpC, the Enzyme Responsible for Bioconversion of Diosgenone to Isonuatigenone in Streptomyces virginiae IBL-14

2009 ◽  
Vol 75 (12) ◽  
pp. 4202-4205 ◽  
Author(s):  
Wei Wang ◽  
Feng-Qing Wang ◽  
Dong-Zhi Wei
Keyword(s):  
Escherichia Coli ◽  
Electron Transfer ◽  
Cytochrome P450 ◽  
Cytochrome P450 Monooxygenase ◽  
P450 Monooxygenase ◽  
Whole Cell ◽  
Electron Transfer Chain ◽  
Streptomyces Virginiae ◽  
Transfer Chain

ABSTRACT A new cytochrome P450 monooxygenase, FcpC, from Streptomyces virginiae IBL-14 has been identified. This enzyme is found to be responsible for the bioconversion of a pyrano-spiro steroid (diosgenone) to a rare nuatigenin-type spiro steroid (isonuatigenone), which is a novel C-25-hydroxylated diosgenone derivative. A whole-cell P450 system was developed for the production of isonuatigenone via the expression of the complete three-component electron transfer chain in an Escherichia coli strain.

Application of a new versatile electron transfer system for cytochrome P450-based Escherichia coli whole-cell bioconversions

2012 ◽  
Vol 97 (17) ◽  
pp. 7741-7754 ◽  
Author(s):  
Michael Ringle ◽  
Yogan Khatri ◽  
Josef Zapp ◽  
Frank Hannemann ◽  
Rita Bernhardt
Keyword(s):  
Escherichia Coli ◽  
Electron Transfer ◽  
Cytochrome P450 ◽  
Transfer System ◽  
Whole Cell ◽  
Electron Transfer System
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