Synthetic seed production from somatic embryos of Pinus radiata

2008 ◽  
Vol 30 (10) ◽  
pp. 1847-1852 ◽  
Author(s):  
Felipe Aquea ◽  
María Josefina Poupin ◽  
José Tomás Matus ◽  
Marlene Gebauer ◽  
Consuelo Medina ◽  
...  
Keyword(s):  
Seed Production ◽  
Pinus Radiata ◽  
Somatic Embryos ◽  
Synthetic Seed

scholarly journals Recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. as a source for synthetic seed production for medium-term preservation

2012 ◽  
Vol 64 (2) ◽  
pp. 809-817 ◽  
Author(s):  
Irina Holobiuc ◽  
R. Catana
Keyword(s):  
Somatic Embryogenesis ◽  
Seed Production ◽  
Somatic Embryos ◽  
Plant Material ◽  
Synthetic Seed ◽  
Sugar Alcohols ◽  
Medium Term ◽  
Gentiana Lutea

Our aim was to establish an efficient and reproducible system for producing synthetic seeds from recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. This species is a vulnerable medicinal plant, protected both at the national and international levels, and is included in different Red Lists and Books. In vitro culture, as an alternative to classical methods of preservation, allows for the cyclic multiplication of plant material and short-, medium- and long-term preservation of tissue collections. Biotechnological approaches allow for maintenance of the plant material in a confined space and protection against biotic and abiotic factors. Somatic embryogenesis (SE) is the most efficient way to regenerate plants, ensuring material for preservation and fundamental research. In our experiment, recurrent somatic embryogenesis was developed in long-term cultures in the presence of sugar alcohols (mannitol, sorbitol) and in the absence of growth factors. This process proceeded at a high rate, with adventive somatic embryos being generated in a continuous process, followed by maturation, germination and development into plants. To follow the somatic embryogenesis process, histological samples were made. We used these embryogenic cultures for synthetic seed production and medium-term conservation. The viability of somatic embryos after moderate osmotic stress treatment was tested using TTC. Our methodology relied on the induction of somatic embryogenesis in the presence of auxins in the first cycle of in vitro cultures, long-term high embryogenic culture maintenance in the presence of sugar alcohols and synthetic seed production.

High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita

Biologia ◽  
2014 ◽  
Vol 69 (2) ◽  
Author(s):  
Vijay Kumar ◽  
Sheela Chandra
Keyword(s):  
Somatic Embryogenesis ◽  
Seed Production ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Synthetic Seeds ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Swertia Chirayita

AbstractAn efficient protocol for plant regeneration through somatic embryogenesis was established from in vivo leaf explants of Swertia chirayita, a critically endangered medicinal herb. The highest frequency (76%) of embryogenic callus was induced on Murashige & Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L kinetin (Kn) from in vivo leaf explants. Globular somatic embryos were induced and further matured from such embryogenic calli by subsequent culture on the same medium. The highest number of somatic embryos (48.83 ± 4.6) was recovered from embryogenic calli derived from leaf explants after 6 weeks of culture. Synthetic seeds were produced by encapsulating of torpedo stage embryos in sodium alginate (4% W/V) gel, dropped into 100 mM calcium chloride (CaCl2 · 2H2O) solution. The synthetic seeds were germinated on MS medium. The highest frequency of synthetic seed germination (84%) was observed on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA. Regenerants were successfully acclimatized under ex vitro condition. This is the first report on synthetic seed production of S. chirayita. Application of these protocols would be helpful in reducing stress in natural habitat, and in long-term storage of elite genotypes through synthetic seed production.

Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix

2015 ◽  
Vol 37 (5) ◽  
Author(s):  
Saikat Gantait ◽  
Suprabuddha Kundu ◽  
Nasim Ali ◽  
Narayan Chandra Sahu
Keyword(s):  
Medicinal Plants ◽  
Seed Production ◽  
Synthetic Seed

Cryopreservation and synthetic seed production in ornamental flower bulbs (geophytes)

2019 ◽  
pp. 17-28
Author(s):  
B. Sevindik ◽  
T. İzgü ◽  
M. Tütüncü ◽  
Y.Y. Mendi
Keyword(s):  
Seed Production ◽  
Synthetic Seed

Rooting of Pinus radiata somatic embryos: factors involved in the success of the process

2018 ◽  
Vol 30 (1) ◽  
pp. 65-71 ◽  
Author(s):  
I. A. Montalbán ◽  
P. Moncaleán
Keyword(s):  
Pinus Radiata ◽  
Somatic Embryos

scholarly journals MORPHOLOGICAL CHANGES DURING THE DEVELOPMENT OF SOMATIC EMBRYOS OF SAGO (Metroxylon sagu Rottb.)

2016 ◽  
Vol 8 (2) ◽  
pp. 43 ◽  
Author(s):  
Pauline D. Kasi ◽  
Sumaryono Sumaryono
Keyword(s):  
Developmental Stage ◽  
Somatic Embryos ◽  
Large Scale ◽  
Developmental Stages ◽  
Morphological Changes ◽  
Petri Dish ◽  
Synthetic Seed ◽  
Globular Embryos ◽  
Metroxylon Sagu

Development of somatic embryos of sago (Metroxylon sagu Rottb.) on agar-solidified medium are highly varied producing heterogeneous seedlings. Understanding of this phenomenon may help in improving the cultural procedures and conditions of sago<br />somatic embryogenesis to obtain uniform seedlings in a large scale. This experiment was conducted at the laboratory for plant cell culture and micropropagation, Indonesian Biotechnology Research Institute for Estate Crops from January to March 2006 to examine morphological changes i.e. color and development stages of sago during their somatic embryo development on an agar-solidified medium. Twenty single globular somatic embryos of sago with specific color (yellowish, greenish, and reddish) were cultured in a Petri dish supplemented with a solid medium. The medium was a micronutrients-modified MS (MMS) with half strength of macronutrients containing 0.01 mg l-1 ABA, 2 mg l-1 kinetin, 20 g l-1 sucrose, 0.5 g l-1 activated charcoal, and 2 g l-1 gelrite. Parameter observed was the percentage of embryo’s number based on color and developmental stage. The result showed that at the end of 6-week culture passage, most originally greenish (80.8%) and reddish (95.8%) embryos remained unchanged in their colors, whereas almost half of the originally yellowish embryos turned to greenish and only 30%<br />remained yellowish. At the same time, single globular embryos have changed gradually into the next developmental stages, although not all of the embryos were germinated. The initial color of embryo affected the rate of the developmental stage changes. Yellowish and greenish globular embryos developed more rapidly into cotyledon or germinant stages at 58% and 55% respectively, in 6 weeks than the reddish ones (41%). Therefore, the yellowish and greenish embryos are the best sources of material for in vitro mass propagation and synthetic seed production of sago.

scholarly journals Somatic Embryogenesis using Immature Zygotic Embryos from Developing Fruits of Papaya (Carica papaya)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 783E-783
Author(s):  
S.K. Dhir ◽  
U.L. Yadava
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Carica Papaya ◽  
Induction Medium ◽  
Zygotic Embryos ◽  
Synthetic Seed ◽  
Factors Affecting ◽  
Secondary Embryos ◽  
Potential Use

An efficient protocol has been developed for the in vitro multiplication of papaya (Carica papaya L.) through somatic embryogenesis utilizing immature zgotic embryos. Somatic embryos were initiated on MS basel media supplemented with 5 mg·liter–1 2,4-D, 400 mg·liter–1 glutamine, and 6% sucrose. After culturing for 2 months, 65% of the explants became highly embryogenic. Each explant produced 50 to 80 embryos in 4 months on culture induction medium. Frequency of embryogenesis was increased (75 to 150 somatic embryos on 80% explants) upon supplementing medium with 4% maltose as a carbon source and 100 mg·liter–1 L-asparagine. The embryogenic callus appeared yellow and embryos at different stages of development were well-organized. On regular subculturing, these cultures continued to produce secondary embryos. Following their transfer to the hormone-free medium supplemented with 4% maltose, these embryos germinated. The somatic embryogenesis system is rapid, repetitive, and highly proliferative. Thus, this system may have a potential use in the development of synthetic seed and transgenic papaya plants. Details of important factors affecting somatic embryogenesis will be discussed.

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