Rooting of Pinus radiata somatic embryos: factors involved in the success of the process

2018 ◽  
Vol 30 (1) ◽  
pp. 65-71 ◽  
Author(s):  
I. A. Montalbán ◽  
P. Moncaleán
2008 ◽  
Vol 30 (10) ◽  
pp. 1847-1852 ◽  
Author(s):  
Felipe Aquea ◽  
María Josefina Poupin ◽  
José Tomás Matus ◽  
Marlene Gebauer ◽  
Consuelo Medina ◽  
...  

1999 ◽  
Vol 105 (1) ◽  
pp. 155-164 ◽  
Author(s):  
Rakesh Minocha ◽  
Dale R. Smith ◽  
Cathie Reeves ◽  
Kevin D. Steele ◽  
Subhash C. Minocha

Trees ◽  
2015 ◽  
Vol 30 (3) ◽  
pp. 949-958 ◽  
Author(s):  
O. García-Mendiguren ◽  
I. A. Montalbán ◽  
T. Goicoa ◽  
M. D. Ugarte ◽  
P. Moncaleán

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)


Author(s):  
G.G. Cossens ◽  
M.F. Hawke

During the first 20 years of a Pinus radiata tree rotation, tree growth and pasture yield were assessed under a range of tree spacings at Invermay and Akatore, two coastal sites in Eastern Otago. Pasture yield in association with trees thinned to 100 stems per hectare (sph) was comparable to that from open pasture up to a tree age of 12 years. By the 19th year, however, pasture production declined to 63% of open pasture yield at Invermay and to 42% at Akatore. At 200 and 400 sph at Akatore, pasture yield was similar to that from open pasture at tree age 12 years but declined to 27% and 0% of open pasture yield respectively by year 20. At both Invermay and Akatore, the ryegrass and clover content of open pasture was relatively constant throughout the term of the trial. However, both the ryegrass and clover content of pasture beneath trees began to decline by tree age 12 years with a very rapid decline at Akatore in the number of pasture species at 200 sph by the 19th year. No pasture remained at 400 sph, after 19 years. Livestock carrying capacity with sheep on tree treatments at Invermay decreased from 100% of open pasture at year 6 to 60% by year 10. At Akatore, livestock carrying capacity averaged over the 20-year life of the trial was 4.1 stock units per hectare with a maximum of 8.1 stock units at a tree age of 8 years. Tree growth at both sites was similar, averaging between 1 and 1.1 m/year in height over 20 years, with trees at Invermay at 100 sph averaging 9% greater height and diameter growth than at Akatore. Increasing tree stocking from 100 to 200 to 400 sph at Akatore, resulted in increased tree height, but decreased diameter at breast height. A comparison of the East Otago trees with those in a similar trial at Tikitere (Rotorua) 900 km further north indicated that the southern trees were about 6 years later in their growth pattern by tree age 20 years. On both sites, soil pH tended to be lower in the presence of trees and was significantly lower than in open pasture by year 20. The results and comparisons with the Tikitere data suggest that, in an integrated agroforestry regime, there will be livestock grazing under the trees further into the tree rotation in Otago than in North Island sites. However, slower tree growth would result in a longer rotation time to harvest. Current recommendations to farmers are to plant trees on the less productive areas of the farm and adopt a tree stocking rate which fully utilises the site. Keywords: agroforestry, livestock, pasture, Pinus radiata, soil pH, tree stocking


HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 573-576 ◽  
Author(s):  
I.E. Yates ◽  
C.C. Reilly

The influence of stage of fruit development and plant growth regulators on somatic embryogenesis and the relation of cultivar response on somatic embryogenesis and subsequent plant development have been investigated in eight cultivars of pecan [Carya illinoensis (Wangenh.) C. Koch]. Explants from the micropylar region of the ovule were more embryogenic when removed from fruits in the liquid endosperm stage than were intact ovules from less-mature fruits or from cotyledonary segments of more-mature fruits. Explants conditioned on medium containing auxin alone or auxin + cytokinin produced more somatic embryos than medium containing cytokinin alone. Under the conditions of this study, frequency of embryogenesis, as well as the germination of somatic embryos leading to plant development, indicated appreciable variation among cultivars. Plant development was greatest by far from somatic embryos of `Schley' than other cultivars studied.


Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 70
Author(s):  
Ana D. Simonović ◽  
Milana M. Trifunović-Momčilov ◽  
Biljana K. Filipović ◽  
Marija P. Marković ◽  
Milica D. Bogdanović ◽  
...  

Centaurium erythraea (centaury) is a traditionally used medicinal plant, with a spectrum of secondary metabolites with confirmed healing properties. Centaury is an emerging model in plant developmental biology due to its vigorous regenerative potential and great developmental plasticity when cultured in vitro. Hereby, we review nearly two decades of research on somatic embryogenesis (SE) in centaury. During SE, somatic cells are induced by suitable culture conditions to express their totipotency, acquire embryogenic characteristics, and eventually give rise to somatic embryos. When SE is initiated from centaury root explants, the process occurs spontaneously (on hormone-free medium), directly (without the callusing phase), and the somatic embryos are of unicellular origin. SE from leaf explants has to be induced by plant growth regulators and is indirect (preceded by callusing). Histological observations and culture conditions are compared in these two systems. The changes in antioxidative enzymes were followed during SE from the leaf explants. Special focus is given to the role of arabinogalactan proteins during SE, which were analyzed using a variety of approaches. The newest and preliminary results, including centaury transcriptome, novel potential SE markers, and novel types of arabinogalactan proteins, are discussed as perspectives of centaury research.


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