A comparative study to evaluate the effects of antibiotics, plant extracts and fluoride-based toothpaste on the oral pathogens isolated from patients with gum diseases in Pakistan

Oral diseases caused by various microorganisms are common around the world. Scientific research has now been focusing on novel medicines to overcome bacterial resistance and antibiotics side effects; therefore, the current study was designed to assess the efficacy of certain antibiotics, toothpaste, and medicinal plant extracts (Ajuga bracteosa and Curcuma longa) versus the bacterial pathogens isolated from the human oral cavity. A total of 130 samples were collected from Khyber Teaching Hospital Peshawar, Pakistan, among those 27 species isolated, and eight bacterial species were identified from the samples. Among all the bacterial species, Staphylococcus aureus (29.62%) and Proteus mirabilis (22.2%) were found to be more prevalent oral pathogens. In comparison, the least pervasive microbes were Proteus vulgaris, Shigella sonnei, Escherichia coli and Aeromonas hydrophila. The study also suggested that dental problems were more prevalent in males (41-50 years of age) than females. Among the eight antibiotics used in the study, the most promising results were shown by Foxicillin against A. hydrophila. The survey of TP1 revealed that it showed more potent antagonist activity against Proteus vulgaris as compared TP2 and TP3 that might be due to the high content of fluoride. The Curcuma longa showed more significant activity than Ajuga bracteosa (Stem, leaves and root) extracts. The data obtained through this study revealed that antibiotics were more effective for oral bacterial pathogens than toothpaste and plant extracts which showed moderate and low activity, respectively. Therefore, it is suggested that the active compounds in individual medicinal plants like Curcuma longa and Ajuga bracteosa could replace the antibiotics when used in daily routine as tooth cleansers or mouth rinses.

Evaluating the Antifungal Activity of Some Traditional Medicinal Plant Extracts against Alternaria solani (Tomato Early Blight Pathogen) in Ethiopia

Background: Tomato (Lycopersicon esculentum Mill.) belongs to the family Solanaceae. In Ethiopia, control of early blight is largely dependent on fungicidal application. There is a research need to identify effective botanical extracts to control Alternaria solani that cause early blight of tomato and for evaluation of plant extracts through different solvents on the target pathogen. Methods: In vitro experiment was conducted to evaluate the effectiveness of crude extracts of 16 selected medicinal plants against Alternaria solani. Thus, crude extracts were extracted from medicinal plants with different solvents (methanol, ethanol and petroleum at (25%, 50% and 100%) concentrations. The Alternaria solani was isolated from infected tomato leaves showing early blight symptoms. Evaluation of plant extracts was carried out against Alternaria solani using food poisoned technique on PDA. Result: Results showed that most of the methanolic extract plants were showed significant inhibition of the mycelial growth as compared to ethanolic and petroleum ether extracts. A higher rate of mycelial reduction was recorded by ethanol extracts of Allium sativum at all concentrations (100%) followed by methanol extracts of Allium sativum at 25%, 50%, 100% concentration (90.02%, 97.01%, 100% respectively). The effectiveness of extracts against Alternaria solani depends on use at the higher concentrations and various solvents. For crude extracts that have shown higher inhibitory effects against Alternaria solani in vitro conditions, actual chemical compounds should be identified. Furthermore, it is also important to evaluate these plants on other microbes, study to test in vivo and to assess their real potential field condition wherever early blight is an important disease of tomato.

Medicinal herbs as a panacea for biogenic silver nanoparticles

Background Scientists created a new area known as "green nanotechnology" by combining the concept of sustainability with nanotechnology. Its goal is to eliminate the use of chemicals in nanoparticle manufacturing by replacing them with plant-based materials. Green synthesis is promoted as the best alternative to the traditional method of nanoparticle synthesis in this new domain. Plants that constitute a major portion of our biodiversity are embraced with inherent potentiality to be transformed as miracle medicine due to its phytochemicals. These phytochemicals efficiently replace the classical wet chemical ingredients involved in nanoparticle synthesis by upgrading to greener method for its synthesis. By incorporating plant-based sources as the chief ingredient of nanoparticle synthesis, we are able to reduce the hazards of greenhouse gas emissions and enlighten the insights of our scientific community with nanotechnology for green innovation. Hence, this review simultaneously aims at promoting plant extracts as the most efficient as well as renewable recipe for green synthesis of silver nanoparticles and preparing earth for a greener tomorrow. Methodology Scientific articles and publications were selected from reputed journals and sorted out with pertinent keywords of this review. Electronic sources like Google Scholar, PubMed, Research Gate, Science Direct, Wiley Online Library, Web of Science and Scopus were searched for potential articles and recent breakthroughs published in the area of silver nanoparticle synthesis via green chemistry and biological methods using plant extracts. Scientific names of medicinal plants were checked using botanical databases like Plant List and International Plant Names Index. Conclusion This review pinpoints on empowering better life on earth by protecting it from hazardous effects of conventional nanotechnological production through replacing the former with sustainable green synthesis approach. Ergo, it outlines that by incorporating plant-based sources as the chief ingredient of nanoparticle synthesis, we are able to reduce the hazards of greenhouse gas emissions in turn by slowing down increasing climate change disasters globally and enlighten the insights of our scientific community with nanotechnology for green innovation.

The Effects of a Gum Arabic-Based Edible Coating on Guava Fruit Characteristics during Storage

Guava is a nutritious fruit that has perishable behavior during storage. We aimed to determine the influences of some edible coatings (namely, cactus pear stem (10%), moringa (10%), and henna leaf (3%) extracts incorporated with gum Arabic (10%)), on the guava fruits’ properties when stored under ambient and refrigeration temperatures for 7, 14, and 21 days. The results revealed that the coating with gum Arabic (10%) only, or combined with the natural plant extracts, exhibited a significant reduction in weight loss, decay, and rot ratio. Meanwhile, there were notable increases in marketability. Moreover, among all tested treatments, the application of gum Arabic (10%) + moringa extract (10%) was the superior treatment for most studied parameters, and exhibited for the highest values for maintaining firmness, total soluble solids, total sugars, and total antioxidant activity. Overall, it was suggested that coating guava with 10% gum Arabic combined with other plant extracts could maintain the postharvest storage quality of the cold-storage guava.

Biogenic Sulfur-Based Chalcogenide Nanocrystals: Methods of Fabrication, Mechanistic Aspects, and Bio-Applications

Bio-nanotechnology has emerged as an efficient and competitive methodology for the production of added-value nanomaterials (NMs). This review article gathers knowledge gleaned from the literature regarding the biosynthesis of sulfur-based chalcogenide nanoparticles (S-NPs), such as CdS, ZnS and PbS NPs, using various biological resources, namely bacteria, fungi including yeast, algae, plant extracts, single biomolecules, and viruses. In addition, this work sheds light onto the hypothetical mechanistic aspects, and discusses the impact of varying the experimental parameters, such as the employed bio-entity, time, pH, and biomass concentration, on the obtained S-NPs and, consequently, on their properties. Furthermore, various bio-applications of these NMs are described. Finally, key elements regarding the whole process are summed up and some hints are provided to overcome encountered bottlenecks towards the improved and scalable production of biogenic S-NPs.

Special Issue on “Phenolic Compounds: Extraction, Optimization, Identification and Applications in Food Industry”

Interest has grown regarding natural plant extracts in food and beverage applications, their vital role in food quality and technology, and their therapeutic use in inhibiting several diseases [...]

Anti-Quorum Sensing Effects of Some Medicinal and Aromatic Plant Extracts on Xanthomonas axonopodis pv. Phaseoli

Xanthomonas axonopodis pv. phaseoli (Xap) is known as one of the most important seed-borne destructive pathogens on beans worldwide. Nowadays, Xap is considered to acquire resistance to antibiotics and synthetic bactericides which concerns the scientific world for its future management. This has made the use of plant extracts, the best alternative in the control of plant disease pathogens by inhibiting the quorum sensing (QS) mediated virulence factors. This research was designed to investigate the antibacterial activities and the anti-QS effects of the 14 different aromatic and medicinal plants against QS-mediated virulence factors of Xap. The results revealed that Syzgium aromaticum showed the largest inhibition zone diameter and strongest antimicrobial (antibacterial) effect among the 14 plant extracts followed by Thymus vulgaris and Coriandrum sativum. Similarly, the lowest swarming, swimming, and twitching motility values were measured from the Syzgium aromaticum application followed by Coriandrum sativum, Thymus vulgaris, Brassica nigra, Lepidium sativum, and Ruta chalepenis. These results indicated that S. aromaticum, C. sativum, T. vulgaris, B. nigra, L. sativum, and R. chalepenis will be a potential candidates as anti-quorum sensing agents in preventing common bacterial disease of beans caused by Xap. Compounds derived from aromatic and medicinal plants have demonstrated successful control of diseases in crops and the use of these substances provides a valuable tool to the growers around the world for diseases management in organic production.

Licorice (Glycyrrhiza glabra, G. uralensis, and G. inflata) and Their Constituents as Active Cosmeceutical Ingredients

The interest in plant extracts and natural compounds in cosmetic formulations is growing. Natural products may significantly improve cosmetics performance since they have both cosmetic and therapeutic-like properties, known as cosmeceutical effects. Glycyrrhiza genus, belonging to the Leguminosae family, comprises more than 30 species, widely distributed worldwide. The rhizomes and roots are the most important medicinal parts currently used in pharmaceutical industries and in the production of functional foods and food supplements. In the last few years, the interest in their potential activities in cosmetic formulations has greatly increased. Glycyrrhiza spp. extracts are widely implemented in cosmetic products for their good whitening effect. The biological effects of Glycyrrhiza extracts are especially ascribable to the occurrence of specialized metabolites belonging to the flavonoid class. This review focuses on the botany and the chemistry of the main investigated Glycyrrhiza spp. (G. glabra, G. uralensis, and G. inflata) along with their cosmeceutical activities categorized as skin anti-aging, photoprotective, hair care, and anti-acne. It has been highlighted how, along with Glycyrrhiza extracts, three main flavonoids namely licochalcone A, glabridin, and dehydroglyasperin C are the most investigated compounds. It is noteworthy that other molecules from licorice show potential cosmeceutical effects. These data suggest further investigations to clarify their potential value for cosmetic industries.