Characterization of twenty-six polymorphic microsatellite markers for the fox squirrel (Sciurus niger) and their utility in gray squirrels (Sciurus carolinensis) and red squirrels (Tamiasciurus hudsonicus)

2009 ◽  
Vol 10 (5) ◽  
Author(s):  
Jennifer A. Fike ◽  
Olin E. Rhodes
Keyword(s):  
Microsatellite Markers ◽  
Tamiasciurus Hudsonicus ◽  
Sciurus Carolinensis ◽  
Sciurus Niger ◽  
Red Squirrels ◽  
Fox Squirrel ◽  
Polymorphic Microsatellite

scholarly journals Cutaneous and Systemic Poxviral Disease in Red (Tamiasciurus hudsonicus) and Gray (Sciurus carolinensis) Squirrels

2009 ◽  
Vol 46 (4) ◽  
pp. 667-672 ◽  
Author(s):  
D. S. Bangari ◽  
M. A. Miller ◽  
G. W. Stevenson ◽  
H. L. Thacker ◽  
A. Sharma ◽  
...  
Keyword(s):  
Pulmonary Nodules ◽  
Tamiasciurus Hudsonicus ◽  
Sciurus Carolinensis ◽  
Epithelial Hyperplasia ◽  
Cutaneous Lesions ◽  
Red Squirrels ◽  
Cytoplasmic Inclusions ◽  
Polymerase Chain ◽  
Renal Tubular

From September 2005 through October 2006, fibromatosis was diagnosed in 2 red squirrels (Tamiasciurus hudsonicus) and 1 gray squirrel (Sciurus carolinensis). All 3 squirrels had multifocal to coalescing, tan, firm alopecic cutaneous nodules. Two squirrels also had pulmonary nodules. Histologically, the cutaneous nodules had marked epidermal hyperplasia, with ballooning degeneration of keratinocytes, spongiosis, and eosinophilic cytoplasmic inclusions. The dermis was expanded by proliferation of atypical mesenchymal cells with cytoplasmic inclusions. Additional findings included pulmonary adenomatous hyperplasia with cytoplasmic inclusions, renal tubular epithelial hyperplasia with cytoplasmic inclusions, atypical mesenchymal proliferation in the liver, and atypical mesenchymal proliferation with cytoplasmic inclusions in the seminal vesicles. Ultrastructurally, poxviral particles were observed in skin scrapings and sections of cutaneous and pulmonary nodules. Polymerase chain reaction targeting the highly conserved Leporipoxvirus DNA polymerase gene was positive using DNA extracted from the cutaneous lesions of all 3 squirrels. Nucleotide sequence of the 390 base PCR amplicons was closely related to that of other members of the genus Leporipoxvirus. To the authors' knowledge, this is the first report of cutaneous and systemic poxviral disease in American red squirrels with molecular characterization of the squirrel fibroma virus.

Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Natural Populations ◽  
Conservation Strategy ◽  
Sequencing Data ◽  
Germplasm Collections ◽  
The Family ◽  
Conservation Genetic ◽  
Population Sizes ◽  
Polymorphic Microsatellite

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattusL.)

2008 ◽  
Vol 8 (4) ◽  
pp. 861-863 ◽  
Author(s):  
T. DAILIANIS ◽  
M. LIMBORG ◽  
R. HANEL ◽  
D. BEKKEVOLD ◽  
J. LAGNEL ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite

Development and characterization of 33 polymorphic microsatellite markers in Trachurus japonicus by SLAF‐seq technology

2020 ◽  
Author(s):  
Su‐Fang Niu ◽  
Yun Zhai ◽  
Ren‐Xie Wu ◽  
Zhen‐Bang Liang ◽  
Hao‐Ran Zhang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Trachurus Japonicus ◽  
Polymorphic Microsatellite

Characterization of polymorphic microsatellite markers in the Dalmatian wall lizardPodarcis melisellensis(Squamata: Lacertidae)

2009 ◽  
Vol 9 (1) ◽  
pp. 299-301 ◽  
Author(s):  
K. HUYGHE ◽  
K. BREUGELMANS ◽  
M. SMALL ◽  
Z. TADIĆ ◽  
R. VAN DAMME ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite

Development and characterization of polymorphic microsatellite markers in Dysosma pleiantha (Berberidaceae)

2011 ◽  
Vol 98 (8) ◽  
pp. e210-e212 ◽  
Author(s):  
Bi-Cai Guan ◽  
Xi Gong ◽  
Shi-Liang Zhou
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite
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