Development and characterization of new polymorphic microsatellite markers in four sea anemones: Entacmaea quadricolor, Heteractis magnifica, Stichodactyla gigantea, and Stichodactyla mertensii

2016 ◽  
Vol 48 (3) ◽  
pp. 1283-1290 ◽  
Author(s):  
Remy Gatins ◽  
Pablo Saenz-Agudelo ◽  
Anna Scott ◽  
Michael L. Berumen
Keyword(s):  
Microsatellite Markers ◽  
Sea Anemones ◽  
Heteractis Magnifica ◽  
Polymorphic Microsatellite ◽  
Stichodactyla Mertensii ◽  
Entacmaea Quadricolor

Extraction and characterization of sea anemones compound and its Anti bacterial and hemolytic studies

2020 ◽  
Vol 10 (4) ◽  
pp. 93-97
Author(s):  
Anil Kumar A ◽  
Raja Sheker K ◽  
Naveen B ◽  
Abhilash G ◽  
Akila CR
Keyword(s):  
Antibacterial Activity ◽  
Focal Point ◽  
Haemolytic Activity ◽  
Klebsiella Pneumonia ◽  
Bacterial Strains ◽  
Sea Anemones ◽  
Marine Plants ◽  
Heteractis Magnifica ◽  
Trididemnum Solidum

Seas assets that give us a variety of characteristic items to control bacterial, contagious and viral ailment and mostly utilized for malignancy chemotherapy practically from spineless creatures, for example, bryozoans, wipes, delicate corals, coelenterates, ocean fans, ocean bunnies, molluscs and echinoderms. In the previous 30 - 40 years, marine plants and creatures have been the focal point of overall endeavours to characterize the regular results of the marine condition. Numerous marine characteristic items have been effectively exceptional to the last phases of clinical preliminaries, including dolastatin-10, a group of peptides disengaged from Indian ocean rabbit, Dollabella auricularia. Ecteinascidin-743 from mangrove tunicate Ecteinascidia turbinata, Didemnins was isolated from Caribbean tunicate Trididemnum solidum and Conopeptides from cone snails (Conus sp.), and a developing number of up-and-comers have been chosen as promising leads for expanded pre-clinical appraisals. Sea anemones possess numerous tentacles containing stinging cells or cnidocytes. The stinging cells are equipped with small organelles known as nematocysts. The two species of sea anemones namely, Heteractis magnificaandStichodactyla haddoni, were collected from Mandapam coastal waters of Ramanathapuram district, Tamilnadu, India. The Nematocyst was collected and centrifuged, and the supernatant was lyophilized and stored for further analysis. The amount of protein from Heteractis Magnifica and Stichodactyla haddoni was estimated. The crude extract has shown haemolytic activity on chicken blood and goat blood. In the antibacterial activity of the sea anemone against six bacterial strains Staphylococcus aureus, Salmonella typhii, Salmonella paratyphii, Klebsiella pneumonia, Vibrio cholerae, Pseudomonas aeruginosa. Antibacterial activity of H. Magnifica and S.haddoni was measured as the radius of the zone of inhibition.

Genetic characterization of novel polymorphic microsatellite markers for Epilobium nankotaizanense (Onagraceae), an endemic and threatened herb in Taiwan

2021 ◽  
pp. 1-4
Author(s):  
Yu-Wei Tseng ◽  
Chi-Chun Huang ◽  
Chih-Chiang Wang ◽  
Chiuan-Yu Li ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Natural Populations ◽  
Conservation Strategy ◽  
Sequencing Data ◽  
Germplasm Collections ◽  
The Family ◽  
Conservation Genetic ◽  
Population Sizes ◽  
Polymorphic Microsatellite

Abstract Epilobium belongs to the family Onagraceae, which consists of approximately 200 species distributed worldwide, and some species have been used as medicinal plants. Epilobium nankotaizanense is an endemic and endangered herb that grows in the high mountains in Taiwan at an elevation of more than 3300 m. Alpine herbs are severely threatened by climate change, which leads to a reduction in their habitats and population sizes. However, only a few studies have addressed genetic diversity and population genetics. In the present study, we developed a new set of microsatellite markers for E. nankotaizanense using high-throughput genome sequencing data. Twenty polymorphic microsatellite markers were developed and tested on 30 individuals collected from three natural populations. These loci were successfully amplified, and polymorphisms were observed in E. nankotaizanense. The number of alleles per locus (A) ranged from 2.000 to 3.000, and the observed (Ho) and expected (He) heterozygosities ranged from 0.000 to 0.929 and from 0.034 to 0.631, respectively. The developed polymorphic microsatellite markers will be useful in future conservation genetic studies of E. nankotaizanense as well as for developing an effective conservation strategy for this species and facilitating germplasm collections and sustainable utilization of other Epilobium species.

Characterization of nine polymorphic microsatellite markers in sprat (Sprattus sprattusL.)

2008 ◽  
Vol 8 (4) ◽  
pp. 861-863 ◽  
Author(s):  
T. DAILIANIS ◽  
M. LIMBORG ◽  
R. HANEL ◽  
D. BEKKEVOLD ◽  
J. LAGNEL ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite

Development and characterization of 33 polymorphic microsatellite markers in Trachurus japonicus by SLAF‐seq technology

2020 ◽  
Author(s):  
Su‐Fang Niu ◽  
Yun Zhai ◽  
Ren‐Xie Wu ◽  
Zhen‐Bang Liang ◽  
Hao‐Ran Zhang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Trachurus Japonicus ◽  
Polymorphic Microsatellite

Characterization of polymorphic microsatellite markers in the Dalmatian wall lizardPodarcis melisellensis(Squamata: Lacertidae)

2009 ◽  
Vol 9 (1) ◽  
pp. 299-301 ◽  
Author(s):  
K. HUYGHE ◽  
K. BREUGELMANS ◽  
M. SMALL ◽  
Z. TADIĆ ◽  
R. VAN DAMME ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite

Development and characterization of polymorphic microsatellite markers in Dysosma pleiantha (Berberidaceae)

2011 ◽  
Vol 98 (8) ◽  
pp. e210-e212 ◽  
Author(s):  
Bi-Cai Guan ◽  
Xi Gong ◽  
Shi-Liang Zhou
Keyword(s):  
Microsatellite Markers ◽  
Polymorphic Microsatellite

Development and characterization of twenty-two polymorphic microsatellite markers for the leafcutter ant, Acromyrmex lundii, utilizing Illumina sequencing

2013 ◽  
Vol 6 (2) ◽  
pp. 319-322 ◽  
Author(s):  
Christian Rabeling ◽  
Martin Bollazzi ◽  
Maurício Bacci ◽  
Rochelle R. Beasley ◽  
Stacey L. Lance ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Illumina Sequencing ◽  
Polymorphic Microsatellite
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