A real-time PCR assay for detection and quantification of Botrytis cinerea in Pelargonium x hortorum plants, and its use for evaluation of plant resistance

2015 ◽  
Vol 143 (1) ◽  
pp. 159-171 ◽  
Author(s):  
Chiaraluce Moretti ◽  
Mara Quaglia ◽  
Martina Cerri ◽  
Daniela E. Nicosia ◽  
Roberto Buonaurio
Keyword(s):  
Real Time ◽  
Botrytis Cinerea ◽  
Plant Resistance ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Pelargonium X Hortorum ◽  
Detection And Quantification

scholarly journals Detection of Peanut Allergen Traces with a Real Time PCR Assay - The Challenge to Protect Food-Allergic Consumers

2017 ◽  
Vol 7 (1) ◽  
pp. 32 ◽  
Author(s):  
Dimitra Houhoula ◽  
Stamatios Koussissis ◽  
Vladimiros Lougovois ◽  
John Tsaknis ◽  
Dimitra Kassavita ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Products ◽  
Molecular Techniques ◽  
Food Allergens ◽  
Pcr Assay ◽  
Peanut Allergen ◽  
Pcr Method ◽  
Detection And Quantification

The aim of the present study was the implementation of molecular techniques in the detection and quantification of allergic substances of peanut in various kinds of food products, e.g., breakfast cereals, chocolates and biscuits that are frequently related to allergies. In some cases, the presence of peanuts can be due to contamination during production and are not declared on the label. A total of 152 samples were collected from supermarkets and were analysed by a Real Time PCR method. The results indicated that 125 samples (83,3%) were found positive in peanut traces but the most important finding is that from the 84 samples that had no allergen declaration for peanuts, 48 (57,1%) of them were found positive. In conclusion, Real Time PCR can be a very important tool for the rapid detection and quantification of food allergens.

scholarly journals One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil

2018 ◽  
Vol 22 (5) ◽  
pp. 418-423
Author(s):  
Elisabete Andrade ◽  
Daniele Rocha ◽  
Marcela Fontana-Maurell ◽  
Elaine Costa ◽  
Marisa Ribeiro ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
One Step ◽  
Detection And Quantification

A real time PCR assay for the detection and quantification of orf virus

2006 ◽  
Vol 134 (1-2) ◽  
pp. 140-145 ◽  
Author(s):  
L. Gallina ◽  
F. Dal Pozzo ◽  
C.J. Mc Innes ◽  
G. Cardeti ◽  
A. Guercio ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Orf Virus ◽  
Pcr Assay ◽  
Detection And Quantification

scholarly journals Real-Time PCR Assay for Detection and Quantification of Hepatitis B Virus Genotypes A to G

2006 ◽  
Vol 44 (9) ◽  
pp. 3325-3333 ◽  
Author(s):  
T. M. Welzel ◽  
W. J. Miley ◽  
T. L. Parks ◽  
J. J. Goedert ◽  
D. Whitby ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
B Virus ◽  
Virus Genotypes ◽  
Detection And Quantification

scholarly journals Development of a real-time PCR assay for direct detection and quantification of Bacillus sporothermodurans in ultra-high temperature milk

2016 ◽  
Vol 99 (10) ◽  
pp. 7864-7871
Author(s):  
M. Abouelnaga ◽  
A. Lamas ◽  
J.M. Miranda ◽  
M. Osman ◽  
A. Cepeda ◽  
...  
Keyword(s):  
High Temperature ◽  
Real Time ◽  
Real Time Pcr ◽  
Direct Detection ◽  
Pcr Assay ◽  
Detection And Quantification ◽  
Ultra High Temperature

scholarly journals Development of a Real-time PCR Assay for the Detection and Quantification of Red Seabream Iridovirus (RSIV).

2003 ◽  
Vol 38 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Christopher Marlowe Caipang ◽  
Ikuo Hirono ◽  
Takashi Aoki
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay ◽  
Red Seabream ◽  
Detection And Quantification
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