Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia

2020 ◽  
Vol 157 (3) ◽  
pp. 685-691
Author(s):  
A. Prokić ◽  
N. Zlatković ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
K. Gašić ◽  
...  
Keyword(s):  
Soft Rot ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Nip, Necrosis-Inducing Virulence Protein of Erwinia carotovora subsp. carotovora

2004 ◽  
Vol 17 (12) ◽  
pp. 1366-1375 ◽  
Author(s):  
Laura Mattinen ◽  
Marina Tshuikina ◽  
Andres Mäe ◽  
Minna Pirhonen
Keyword(s):  
Leaf Tissue ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bacterial Cells ◽  
Virulence Determinants ◽  
Virulence Protein ◽  
Encoding Gene ◽  
Rot Disease ◽  
Identification And Characterization

Erwinia carotovora subsp. carotovora is a gram-negative bacterium that causes soft rot disease of many cultivated crops. When a collection of E. carotovora subsp. carotovora isolates was analyzed on a Southern blot using the harpin-encoding gene hrpN as probe, several harpinless isolates were found. Regulation of virulence determinants in one of these, strain SCC3193, has been characterized extensively. It is fully virulent on potato and in Arabidopsis thaliana. An RpoS (SigmaS) mutant of SCC3193, producing elevated levels of secreted proteins, was found to cause lesions resembling the hypersensitive response when infiltrated into tobacco leaf tissue. This phenotype was evident only when bacterial cells had been cultivated on solid minimal medium at low pH and temperature. The protein causing the cell death was purified and sequenced, and the corresponding gene was cloned. The deduced sequence of the necrosis-inducing protein (Nip) showed homology to necrosis- and ethylene-inducing elicitors of fungi and oomycetes. A mutant strain of E. carotovora subsp. carotovora lacking the nip gene showed reduced virulence in potato tuber assay but was unaffected in virulence in potato stem or on other tested host plants.

scholarly journals PENGARUH SUMBER MINERAL TERHADAP PENEKANAN Erwinia carotovora OLEH PSEUDOMONAS PENDAR-FLUOR SECARA IN VITRO

2012 ◽  
Vol 7 (2) ◽  
pp. 117-124
Author(s):  
Hardian Susilo Addy
Keyword(s):  
Retention Factor ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Fluorescent Pseudomonad ◽  
Antimicrobial Substances ◽  
Identification And Characterization ◽  
Stimulation Of ◽  
Mineral Sources

Antimicrobial Stimulation of Fluorescent Pseudomonad to Inhibit Soft-rot Pathogen Caused by Erwinia carotovora subsp. Carotovora. This research was conducted to study effect of mineral sources on inhibition Erwinia carotovora by fluorescent pseudomonad. We used several mineral sources to stimulate antimicrobial substances from fluorescent pseudomonad that responsible to inhibit E. carotovora subsp. carotovora in vitro. The results showed that zinc 0,5 mM were the best to increase antagonistics of fluorescent psudomonad againts E. carotovora. Zinc were increased antimicrobial substances twohold compared with control without stimulant agent. Detection of antimicrobial substance using TLC showed that only one antimicrobial was detected with retention factor (Rf) of 0,68 – 0,72. However, identification and characterization of that substance is still needed.

Identification and characterization of peptidases secreted by quahog parasite unknown (QPX), the protistan parasite of hard clams

2016 ◽  
Vol 122 (1) ◽  
pp. 21-33 ◽  
Author(s):  
E Rubin ◽  
GT Werneburg ◽  
E Pales Espinosa ◽  
DG Thanassi ◽  
B Allam
Keyword(s):  
Quahog Parasite Unknown ◽  
Hard Clams ◽  
Identification And Characterization

Identification and characterization of aldose reductase in cultured rat mesangial cells

Diabetes ◽  
1992 ◽  
Vol 41 (9) ◽  
pp. 1165-1171 ◽  
Author(s):  
R. Kikkawa ◽  
K. Umemura ◽  
M. Haneda ◽  
N. Kajiwara ◽  
S. Maeda ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Mesangial Cells ◽  
Identification And Characterization
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