scholarly journals Identification and Characterization of Nip, Necrosis-Inducing Virulence Protein of Erwinia carotovora subsp. carotovora

2004 ◽  
Vol 17 (12) ◽  
pp. 1366-1375 ◽  
Author(s):  
Laura Mattinen ◽  
Marina Tshuikina ◽  
Andres Mäe ◽  
Minna Pirhonen
Keyword(s):  
Leaf Tissue ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Bacterial Cells ◽  
Virulence Determinants ◽  
Virulence Protein ◽  
Encoding Gene ◽  
Rot Disease ◽  
Identification And Characterization

Erwinia carotovora subsp. carotovora is a gram-negative bacterium that causes soft rot disease of many cultivated crops. When a collection of E. carotovora subsp. carotovora isolates was analyzed on a Southern blot using the harpin-encoding gene hrpN as probe, several harpinless isolates were found. Regulation of virulence determinants in one of these, strain SCC3193, has been characterized extensively. It is fully virulent on potato and in Arabidopsis thaliana. An RpoS (SigmaS) mutant of SCC3193, producing elevated levels of secreted proteins, was found to cause lesions resembling the hypersensitive response when infiltrated into tobacco leaf tissue. This phenotype was evident only when bacterial cells had been cultivated on solid minimal medium at low pH and temperature. The protein causing the cell death was purified and sequenced, and the corresponding gene was cloned. The deduced sequence of the necrosis-inducing protein (Nip) showed homology to necrosis- and ethylene-inducing elicitors of fungi and oomycetes. A mutant strain of E. carotovora subsp. carotovora lacking the nip gene showed reduced virulence in potato tuber assay but was unaffected in virulence in potato stem or on other tested host plants.

scholarly journals PENGARUH SUMBER MINERAL TERHADAP PENEKANAN Erwinia carotovora OLEH PSEUDOMONAS PENDAR-FLUOR SECARA IN VITRO

2012 ◽  
Vol 7 (2) ◽  
pp. 117-124
Author(s):  
Hardian Susilo Addy
Keyword(s):  
Retention Factor ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Fluorescent Pseudomonad ◽  
Antimicrobial Substances ◽  
Identification And Characterization ◽  
Stimulation Of ◽  
Mineral Sources

Antimicrobial Stimulation of Fluorescent Pseudomonad to Inhibit Soft-rot Pathogen Caused by Erwinia carotovora subsp. Carotovora. This research was conducted to study effect of mineral sources on inhibition Erwinia carotovora by fluorescent pseudomonad. We used several mineral sources to stimulate antimicrobial substances from fluorescent pseudomonad that responsible to inhibit E. carotovora subsp. carotovora in vitro. The results showed that zinc 0,5 mM were the best to increase antagonistics of fluorescent psudomonad againts E. carotovora. Zinc were increased antimicrobial substances twohold compared with control without stimulant agent. Detection of antimicrobial substance using TLC showed that only one antimicrobial was detected with retention factor (Rf) of 0,68 – 0,72. However, identification and characterization of that substance is still needed.

scholarly journals De-Coding the Contributions of the Viral RNAs to Alphaviral Pathogenesis

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 771
Author(s):  
Autumn T. LaPointe ◽  
Kevin J. Sokoloski
Keyword(s):  
Host Response ◽  
Severe Disease ◽  
Review Article ◽  
Structural Proteins ◽  
Virulence Determinants ◽  
Healthy Individuals ◽  
Viral Rnas ◽  
Positive Sense ◽  
Identification And Characterization

Alphaviruses are positive-sense RNA arboviruses that are capable of causing severe disease in otherwise healthy individuals. There are many aspects of viral infection that determine pathogenesis and major efforts regarding the identification and characterization of virulence determinants have largely focused on the roles of the nonstructural and structural proteins. Nonetheless, the viral RNAs of the alphaviruses themselves play important roles in regard to virulence and pathogenesis. In particular, many sequences and secondary structures within the viral RNAs play an important part in the development of disease and may be considered important determinants of virulence. In this review article, we summarize the known RNA-based virulence traits and host:RNA interactions that influence alphaviral pathogenesis for each of the viral RNA species produced during infection. Overall, the viral RNAs produced during infection are important contributors to alphaviral pathogenesis and more research is needed to fully understand how each RNA species impacts the host response to infection as well as the development of disease.

scholarly journals Kemampuan Bakteri Endofit Akar dan Ubi Kentang untuk Menekan Penyakit Busuk Lunak (Erwinia carotovora pv. carotovora) pada Ubi

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Noor Istifadah ◽  
Muhamad Salman Umar ◽  
Sudarjat Sudarjat ◽  
Luciana Djaya
Keyword(s):  
Biological Control ◽  
Potato Tuber ◽  
Endophytic Bacteria ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Soft Rot Disease ◽  
Post Harvest ◽  
Rot Disease

ABSTRACTThe abilities of endophytic bacteria from potato roots and tubers to suppress soft rot disease (Erwinia carotovora pv. carotovora) in potato tuberSoft rot disease caused by Erwinia carotovora pv. carotovora is one of limiting factors in cultivation and post harvest of potato. The eco-friendly control measure that can be developed for controlling the diseases is biological control. Microbes that are potential as biological control agents include endophytic bacteria. This paper discussed the results of study examining the potential of endophytic bacteria isolated from roots and tubers of potato to inhibit the growth of E. carotovora pv. carotovora in vitro and suppress soft rot disease in potato tuber. The results showed that among 24 isolates examined, four isolates of endophytic bacteria (one isolate from potato tuber and three isolates from potato roots) inhibited the growth of E. carotovora pv. carotovora in vitro with inhibition zone 3.5-6.8 mm. In the in vivo test, the isolates inhibited the soft rot disease in potato tuber by 71.5-86.4%. The isolate that tended to show relatively better inhibition in vitro and in vivo was isolate from potato tuber which is CK U3 (Lysinibacillus sp.)Keywords: Biological control, Endophytic bacteria, Post-harvest, Potato, Soft rot diseaseABSTRAKPenyakit busuk lunak yang disebabkan bakteri Erwinia carotovora pv. carotovora, merupakan salah satu kendala dalam budidaya dan pascapanen kentang. Cara pengendalian ramah lingkungan yang dapat dikembangkan untuk menekan penyakit tersebut adalah pengendalian biologi. Kelompok mikroba yang berpotensi sebagi agens pengendali biologi adalah bakteri endofit. Artikel ini mendiskusikan potensi isolat bakteri endofit yang berasal dari ubi dan akar kentang untuk menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dan menekan perkembangan penyakit busuk lunak pada ubi kentang. Hasil percobaan menunjukkan bahwa diantara 24 isolat bakteri yang diuji, terdapat empat isolat bakteri endofit (satu isolat dari ubi kentang dan tiga isolat dari akar kentang) yang dapat menghambat pertumbuhan bakteri E. carotovora pv. carotovora secara in vitro dengan zona penghambatan sebesar 3,5-6,8 mm. Pada pengujian secara in vivo, isolat-isolat tersebut dapat menekan perkembangan penyakit busuk lunak pada ubi kentang sebesar 71,5-86,4%. Isolat yang cenderung menunjukkan penghambatan relatif lebih baik secara in vitro dan in vivo adalah isolat bakteri endofit asal ubi kentang yaitu isolat CK U3 (Lysinibacillus sp.).Kata Kunci: Pengendalian biologi, Bakteri endofit, Pascapanen, Kentang, Penyakit busuk basah

Identification and characterization of Dickeya zeae strains associated with maize stalk soft-rot in northern Serbia

2020 ◽  
Vol 157 (3) ◽  
pp. 685-691
Author(s):  
A. Prokić ◽  
N. Zlatković ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
K. Gašić ◽  
...  
Keyword(s):  
Soft Rot ◽  
Identification And Characterization

Identification and Characterization of a Cellulase-Encoding Gene from the Buffalo Rumen Metagenomic Library

2012 ◽  
Vol 76 (6) ◽  
pp. 1075-1084 ◽  
Author(s):  
Nhung Hong NGUYEN ◽  
Lalita MARUSET ◽  
Tanaporn UENGWETWANIT ◽  
Wuttichai MHUANTONG ◽  
Piyanun HARNPICHARNCHAI ◽  
...  
Keyword(s):  
Metagenomic Library ◽  
Encoding Gene ◽  
Identification And Characterization ◽  
Buffalo Rumen

Identification and characterization of the Cryptococcus neoformans phosphomannose isomerase-encoding gene, MAN1, and its impact on pathogenicity

2001 ◽  
Vol 40 (3) ◽  
pp. 610-620 ◽  
Author(s):  
Elizabeth A. Wills ◽  
Ian S. Roberts ◽  
Maurizio Del Poeta ◽  
Johanna Rivera ◽  
Arturo Casadevall ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Phosphomannose Isomerase ◽  
Encoding Gene ◽  
Identification And Characterization

scholarly journals Erwinia carotovora pv. carotovora, causal agent of soft rot disease in some crops in Puerto Rico

1969 ◽  
Vol 74 (1) ◽  
pp. 83-92
Author(s):  
Amelia Cortés-Monllor
Keyword(s):  
Puerto Rico ◽  
Erwinia Carotovora ◽  
Soft Rot ◽  
Causal Agent ◽  
Bacterial Isolates ◽  
Soft Rot Disease ◽  
Calla Lily ◽  
Pathogenicity Tests ◽  
Rot Disease ◽  
Antibiotic Response

The bacterium Erwinia carotovora pv. carotovora (Jones) was identified from bacterial isolates from decaying material of tobacco, tomato, tanier, head lettuce, cabbage, Dracaena spp, Pothos spp, and calla lily as the causal agent of soft rot disease. Physiological characteristics, biochemical reactions and antibiotic response were similar among strains. The ability of the isolates to produce soft rot symptoms on healthy plants when artificially inoculated was demonstrated by pathogenicity tests.

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