Methane and nitrous oxide emissions from rice paddy soil as influenced by timing of application of hydroquinone and dicyandiamide

2009 ◽  
Vol 85 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Xianglan Li ◽  
Xiaoyan Zhang ◽  
Hua Xu ◽  
Zucong Cai ◽  
Kazuyuki Yagi
Keyword(s):  
Nitrous Oxide ◽  
Paddy Soil ◽  
Rice Paddy ◽  
Nitrous Oxide Emissions ◽  
Rice Paddy Soil

Wood vinegar and biochar co-application mitigates nitrous oxide and methane emissions from rice paddy soil: A two-year experiment

2020 ◽  
Vol 267 ◽  
pp. 115403
Author(s):  
Yanfang Feng ◽  
Detian Li ◽  
Haijun Sun ◽  
Lihong Xue ◽  
Beibei Zhou ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Paddy Soil ◽  
Rice Paddy ◽  
Methane Emissions ◽  
Rice Paddy Soil ◽  
Wood Vinegar

Controlled-Release Urea Commingled with Rice Seeds Reduced Emission of Ammonia and Nitrous Oxide in Rice Paddy Soil

2013 ◽  
Vol 42 (6) ◽  
pp. 1661-1673 ◽  
Author(s):  
Yuechao Yang ◽  
Min Zhang ◽  
Yuncong Li ◽  
Xiaohui Fan ◽  
Yuqing Geng
Keyword(s):  
Nitrous Oxide ◽  
Controlled Release ◽  
Paddy Soil ◽  
Rice Paddy ◽  
Rice Paddy Soil ◽  
Rice Seeds ◽  
Controlled Release Urea

scholarly journals Enhancement of nitrous oxide emissions in soil microbial consortia via copper competition between proteobacterial methanotrophs and denitrifiers

2020 ◽  
pp. AEM.02301-20
Author(s):  
Jin Chang ◽  
Daehyun Daniel Kim ◽  
Jeremy D. Semrau ◽  
Juyong Lee ◽  
Hokwan Heo ◽  
...  
Keyword(s):  
Paddy Soil ◽  
Significant Proportion ◽  
Rice Paddy ◽  
Microbial Consortia ◽  
Nitrous Oxide Emissions ◽  
Soil Slurry ◽  
Rice Paddy Soil ◽  
Methylosinus Trichosporium ◽  
Soil Microbial ◽  
High Affinity

Unique means of copper scavenging have been identified in proteobacterial methanotrophs, particularly the use of methanobactin, a novel ribosomally synthesized post-translationally modified polypeptide that binds copper with very high affinity. The possibility that copper sequestration strategies of methanotrophs may interfere with copper uptake of denitrifiers in situ and thereby enhance N2O emissions was examined using a suite of laboratory experiments performed with rice paddy microbial consortia. Addition of purified methanobactin from Methylosinus trichosporium OB3b to denitrifying rice paddy soil microbial consortia resulted in substantially increased N2O production, with more pronounced responses observed for soils with lower copper content. The N2O emission-enhancing effect of the soil’s native mbnA-expressing Methylocystaceae methanotrophs on the native denitrifiers was then experimentally verified with a Methylocystaceae-dominant chemostat culture prepared from a rice paddy microbial consortium as the inoculum. Lastly, with microcosms amended with varying cell numbers of methanobactin-producing Methylosinus trichosporium OB3b before CH4 enrichment, microbiomes with different ratios of methanobactin-producing Methylocystaceae to gammaproteobacterial methanotrophs incapable of methanobactin production were simulated. Significant enhancement of N2O production from denitrification was evident in both Methylocystaceae-dominant and Methylococcaceae-dominant enrichments, albeit to a greater extent in the former, signifying the comparative potency of methanobactin-mediated copper sequestration while implying the presence of alternative copper abstraction mechanisms for Methylococcaceae. These observations support that copper-mediated methanotrophic enhancement of N2O production from denitrification is plausible where methanotrophs and denitrifiers cohabit.IMPORTANCE Proteobacterial methanotrophs, groups of microorganisms that utilize methane as source of energy and carbon, have been known to utilize unique mechanisms to scavenge copper, namely utilization of methanobactin, a polypeptide that binds copper with high affinity and specificity. Previously the possibility that copper sequestration by methanotrophs may lead to alteration of cuproenzyme-mediated reactions in denitrifiers and consequently increase emission of potent greenhouse gas N2O has been suggested in axenic and co-culture experiments. Here, a suite of experiments with rice paddy soil slurry cultures with complex microbial compositions were performed to corroborate that such copper-mediated interplay may actually take place in environments co-habited by diverse methanotrophs and denitrifiers. As spatial and temporal heterogeneity allow for spatial coexistence of methanotrophy (aerobic) and denitrification (anaerobic) in soils, the results from this study suggest that this previously unidentified mechanism of N2O production may account for significant proportion of N2O efflux from agricultural soils.

scholarly journals Enhancement of nitrous oxide emissions in soil microbial consortia via copper competition between proteobacterial methanotrophs and denitrifiers

2020 ◽  
Author(s):  
Jin Chang ◽  
Daehyun Daniel Kim ◽  
Jeremy D. Semrau ◽  
Juyong Lee ◽  
Hokwan Heo ◽  
...  
Keyword(s):  
Paddy Soil ◽  
Significant Proportion ◽  
Rice Paddy ◽  
Microbial Consortia ◽  
Nitrous Oxide Emissions ◽  
Soil Slurry ◽  
Rice Paddy Soil ◽  
Methylosinus Trichosporium ◽  
Soil Microbial ◽  
High Affinity

AbstractUnique means of copper scavenging have been identified in proteobacterial methanotrophs, particularly the use of methanobactin, a novel ribosomally synthesized post-translationally modified polypeptide that binds copper with very high affinity. The possibility that copper sequestration strategies of methanotrophs may interfere with copper uptake of denitrifiers in situ and thereby enhance N2O emissions was examined using a suite of laboratory experiments performed with rice paddy microbial consortia. Addition of purified methanobactin from Methylosinus trichosporium OB3b to denitrifying rice paddy soil microbial consortia resulted in substantially increased N2O production, with more pronounced responses observed for soils with lower copper content. The N2O emission-enhancing effect of the soil’s native mbnA-expressing Methylocystaceae methanotrophs on the native denitrifiers was then experimentally verified with a Methylocystaceae-dominant chemostat culture prepared from a rice paddy microbial consortium as the inoculum. Lastly, with microcosms amended with varying cell numbers of methanobactin-producing Methylosinus trichosporium OB3b before CH4 enrichment, microbiomes with different ratios of methanobactin-producing Methylocystaceae to gammaproteobacterial methanotrophs incapable of methanobactin production were simulated. Significant enhancement of N2O production from denitrification was evident in both Methylocystaceae-dominant and Methylococcaceae-dominant enrichments, albeit to a greater extent in the former, signifying the comparative potency of methanobactin-mediated copper sequestration while implying the presence of alternative copper abstraction mechanisms for Methylococcaceae. These observations support that copper-mediated methanotrophic enhancement of N2O production from denitrification is plausible where methanotrophs and denitrifiers cohabit.ImportanceProteobacterial methanotrophs, groups of microorganisms that utilize methane as source of energy and carbon, have been known to utilize unique mechanisms to scavenge copper, namely utilization of methanobactin, a polypeptide that binds copper with high affinity and specificity. Previously the possibility that copper sequestration by methanotrophs may lead to alteration of cuproenzyme-mediated reactions in denitrifiers and consequently increase emission of potent greenhouse gas N2O has been suggested in axenic and co-culture experiments. Here, a suite of experiments with rice paddy soil slurry cultures with complex microbial compositions were performed to corroborate that such copper-mediated interplay may actually take place in environments co-habited by diverse methanotrophs and denitrifiers. As spatial and temporal heterogeneity allow for spatial coexistence of methanotrophy (aerobic) and denitrification (anaerobic) in soils, the results from this study suggest that this previously unidentified mechanism of N2O production may account for significant proportion of N2O efflux from agricultural soils.

Mechanical insights into the effect of fluctuation in soil moisture on nitrous oxide emissions from paddy soil

2016 ◽  
Vol 15 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Lan Ma ◽  
Yi Cheng ◽  
Jinyang Wang ◽  
Xiaoyuan Yan
Keyword(s):  
Nitrous Oxide ◽  
Soil Moisture ◽  
Paddy Soil ◽  
Nitrous Oxide Emissions

Water-washed hydrochar in rice paddy soil reduces N2O and CH4 emissions: A whole growth period investigation

2021 ◽  
Vol 274 ◽  
pp. 116573
Author(s):  
Danyan Chen ◽  
Yibo Zhou ◽  
Cong Xu ◽  
Xinyu Lu ◽  
Yang Liu ◽  
...  
Keyword(s):  
Paddy Soil ◽  
Growth Period ◽  
Rice Paddy ◽  
Rice Paddy Soil ◽  
Ch4 Emissions
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