Erratum to: Synthetic seed technology for short term conservation of medicinal orchid Dendrobium densiflorum Lindl. Ex Wall and assessment of genetic fidelity of regenerants

Abstract Synthetic seed were produced from protocorm-like bodies (PLBs) of hybrid Cymbidium Twilight Moon ‘Day Light’ after culture on a new medium, Teixeira Cymbidium (TC) medium. This new medium contained, in addition to a unique selection of macro- and micronutrients, 0.1 mg/l α-naphthaleneacetic acid and 0.1 mg/l kinetin, 2 g/l tryptone and 20 g/l sucrose, and was solidified with 8 g/l Bacto agar. Several explant types and sizes (intact PLBs, half-PLBs, PLB longitudinal thin cell layers) were tested. In addition, pretreatment of PLB-synseeds with 200 mM KNO3 solution, the addition of activated charcoal or coconut water to synseeds, light vs dark culture, short-term (1 month) and long-term (6 and 12 months) low-temperature (4°C) storage, as well as cryostorage were also tested. All treatments resulted in less PLBs than the control treatment. Among all these treatments, only the use of TC medium or incorporation of coconut water into synseeds resulted in “germination” while lowtemperature storage (1-6 months) was only possible under liquid TC. These results would allow for the short-term preservation of Cymbidium germplasm but not for effective cryopreservation.

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Synthetic seed technology for encapsulation and regrowth of in vitro-derived Acacia hyrid shoot and axillary buds

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.492 ◽

2011 ◽

Vol 10 (40) ◽

pp. 7820-7824 ◽

Cited By ~ 11

Author(s):

Asmah Nor ◽

H ◽

Hasnida Nor ◽

H ◽

Zaimah Nashatul ◽

...

Keyword(s):

Axillary Buds ◽

Synthetic Seed ◽

Seed Technology

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Micropropagation of Cymbidium aloifolium (L.) SW., A Medicinal Orchid by Artificial Seeds Technology

Journal of Natural History Museum ◽

10.3126/jnhm.v28i0.14166 ◽

2015 ◽

Vol 28 ◽

pp. 42-48 ◽

Cited By ~ 2

Author(s):

Shreeti Pradhan ◽

Babu Lal Tiruwa ◽

Bijay R Subedee ◽

Bijaya Pant

Keyword(s):

Basal Medium ◽

Mass Scale ◽

Scale Production ◽

Ms Medium ◽

Artificial Seeds ◽

Seed Technology ◽

Artificial Seed ◽

Cymbidium Aloifolium ◽

Medicinal Orchid

Artificial seed technology is a rapidly growing area of research in plant cell and tissue culture. Application of this technology opens an alternative route for mass scale production, efficient delivery of cloned plantlets and fulfils the increasing demand of local growers. An attempt was made to produce artificial seeds and their subsequent regeneration of a highly valuable medicinal orchid of Nepal i.e. Cymbidium aloifolium. Artificial seeds were obtained through encapsulation of protocorms in calcium alginate beads. Protocorms were encapsulated by using 3% sodium alginate and 0.2 M calcium chloride solution. Murashige and Skoog (MS) medium (1962) was used as the basal medium for in vitro germination and seedling development of artificial seed. In Cymbidium aloifolium, 20-25 days old in vitro grown protocorms were used for production of artificial seeds. Artificial seeds were inoculated on two different culture conditions of MS medium i.e. MS solid & MS liquid with four different treatments i.e. strength of 1.0, ½, ¼ and MS media supplemented with plant growth regulators viz. BAP (0.5 mg/l) and NAA (0.5 mg/l). Highest percentage of germination (100%) and plantlet conversion was found on hormone free full strength (1.0 MS) of MS liquid medium after 13-14 weeks of culture. Plantlets regenerated from artificial seeds with well developed shoot and root systems were successfully acclimatized in potting mixture of cocopeat, litter and sphagnum moss in a ratio 2:1:1.J. Nat. Hist. Mus. Vol. 28, 2014: 42-48

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