Styryl Dyes as Two-Photon Excited Fluorescent Probes for DNA Detection and Two-Photon Laser Scanning Fluorescence Microscopy of Living Cells

2010 ◽  
Vol 20 (4) ◽  
pp. 865-872 ◽  
Author(s):  
Valentyna P. Tokar ◽  
Mykhaylo Yu. Losytskyy ◽  
Tymish Y. Ohulchanskyy ◽  
Dmytro V. Kryvorotenko ◽  
Vladyslava B. Kovalska ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescent Probes ◽  
Laser Scanning ◽  
Dna Detection ◽  
Living Cells ◽  
Styryl Dyes ◽  
Two Photon

Theoretical investigation and design of two-photon fluorescent probes for visualizing β-galactosidase activity in living cells

RSC Advances ◽  
2016 ◽  
Vol 6 (75) ◽  
pp. 70960-70971 ◽  
Author(s):  
Chun Zhang ◽  
Dan Wang ◽  
Li Zhang ◽  
Jing-Fu Guo ◽  
Ai-Min Ren
Keyword(s):  
Theoretical Investigation ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Galactosidase Activity ◽  
Two Photon ◽  
Bio Imaging

The two-photon fluorescent probes show dual signal for β-gal bio-imaging.

Imaging nucleus viscosity and G-quadruplex DNA in living cells using a nucleus-targeting two-photon fluorescent probe

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5799-5804 ◽  
Author(s):  
Wan Sun ◽  
Jing-Xue Cui ◽  
Le-Le Ma ◽  
Zhong-Lin Lu ◽  
Bing Gong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Dna Detection ◽  
Living Cells ◽  
Cell Nuclei ◽  
Quadruplex Dna ◽  
Two Photon ◽  
Turn On ◽  
G Quadruplex

TP-2Bz, as a nucleus targeting “D–π–A” molecule, was developed as a two-photon turn-on fluorescent probe for the viscosity and GQ DNA detection in cell nuclei.

Cyanines as New Fluorescent Probes for DNA Detection and Two-Photon Excited Bioimaging

2010 ◽  
Vol 12 (10) ◽  
pp. 2194-2197 ◽  
Author(s):  
Xin Jiang Feng ◽  
Po Lam Wu ◽  
Frédéric Bolze ◽  
Heidi W. C. Leung ◽  
King Fai Li ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Dna Detection ◽  
Two Photon

Imaging Subcellular Changes in Living Mammalian Embryos Using 1047 Nm Two Photon Excitation Fluorescence Microscopy

1999 ◽  
Vol 5 (S2) ◽  
pp. 1060-1061
Author(s):  
J. M. Squirrell ◽  
D. L. Wokosin ◽  
B. D. Bavister ◽  
J. G. White
Keyword(s):  
Fluorescence Imaging ◽  
Laser Scanning ◽  
Prolonged Exposure ◽  
Laser Scanning Confocal Microscopy ◽  
Living Cells ◽  
Laser Scanning Microscopy ◽  
Scanning Confocal Microscopy ◽  
Two Photon ◽  
Photon Excitation ◽  
Mammalian Embryos

A major challenge for fluorescence imaging of living cells is maintaining viability during and following prolonged exposure to excitation illumination, especially when imaging over hours or even days, as when studying mammalian embryonic development. The use of specific fluorescently labeled components in living embryos promises to reveal the roles of organelles and molecules in a native and reproducible context. However, to gain a thorough understanding of dynamic biological systems, events of interest must be recorded as they occur, while limiting perturbations caused by the observation technique. Therefore, establishing long-term fluorescence imaging methods that maintain viability is critical for advancing our understanding of cell and developmental biology.One promising technique for imaging living cells is two photon laser scanning microscopy (TPLSM). The lower energy per photon and the restriction of fluorophore excitation to the imaged focal plane should reduce the total photodamage to thick specimens when compared to conventional laser scanning confocal microscopy (LSCM).

Indolenine-Based Derivatives as Customizable Two-Photon Fluorescent Probes for pH Bioimaging in Living Cells

ACS Sensors ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1068-1074
Author(s):  
Carlos Benitez-Martin ◽  
Juan A. Guadix ◽  
John R. Pearson ◽  
Francisco Najera ◽  
Jose M. Perez-Pomares ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Living Cells ◽  
Two Photon

Two‐photon laser scanning fluorescence microscopy‐from a fluorophore and specimen perspective

Bioimaging ◽  
1996 ◽  
Vol 4 (3) ◽  
pp. 168-178 ◽  
Author(s):  
J D Bhawalkar ◽  
A Shih ◽  
S J Pan ◽  
W S Liou ◽  
J Swiatkiewicz ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Laser Scanning ◽  
Two Photon

Two-Photon Excited Luminescent Styryl Dyes as Probes for the DNA Detection and Imaging. Photostability and Phototoxic Influence on DNA

2007 ◽  
Vol 467 (1) ◽  
pp. 325-338 ◽  
Author(s):  
V. M. Yashchuk ◽  
V. V. Gusak ◽  
I. M. Dmytruk ◽  
V. M. Prokopets ◽  
V. Yu. Kudrya ◽  
...  
Keyword(s):  
Dna Detection ◽  
Styryl Dyes ◽  
Two Photon

Design and synthesis of fluorenone-based dyes: two-photon excited fluorescent probes for imaging of lysosomes and mitochondria in living cells

2015 ◽  
Vol 3 (16) ◽  
pp. 3315-3323 ◽  
Author(s):  
A. L. Capodilupo ◽  
V. Vergaro ◽  
E. Fabiano ◽  
Milena De Giorgi ◽  
F. Baldassarre ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probes ◽  
Living Cells ◽  
Design And Synthesis ◽  
Two Photon ◽  
Specific Targeting

Three fluorenone-based two-photon fluorescent probes for specific targeting of lysosomes and mitochondria in cancer cells.

scholarly journals Turn-on fluorescent probes that can light up endogenous RNA in nucleoli and cytoplasm of living cells under a two-photon microscope

RSC Advances ◽  
2017 ◽  
Vol 7 (27) ◽  
pp. 16730-16736 ◽  
Author(s):  
Ruiqing Feng ◽  
Longlong Li ◽  
Bing Li ◽  
Jinhui Li ◽  
Dan Peng ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Living Cells ◽  
Two Photon ◽  
Turn On ◽  
Rna Probes

We report two turn-on RNA probes that can light up endogenous RNA in the nucleoli and cytoplasm of living cells under a two-photon microscope.

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