Rice blast resistance gene Pikahei-1(t), a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein

2014 ◽  
Vol 34 (2) ◽  
pp. 691-700 ◽  
Author(s):  
Xin Xu ◽  
Nagao Hayashi ◽  
Chun-Tai Wang ◽  
Shuichi Fukuoka ◽  
Shinji Kawasaki ◽  
...  
Keyword(s):  
Binding Site ◽  
Gene Cluster ◽  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Chromosome 4 ◽  
Leucine Rich Repeat ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Leucine Rich Repeat Protein

scholarly journals Rice blast resistance gene Pii is controlled by a pair of NBS-LRR genes Pii-1 and Pii-2

2017 ◽  
Author(s):  
Hiroki Takagi ◽  
Akira Abe ◽  
Aiko Uemura ◽  
Kaori Oikawa ◽  
Hiroe Utsushi ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Gene Sequence ◽  
Blast Resistance ◽  
Rice Cultivar ◽  
Leucine Rich Repeat ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Pathogen Effectors ◽  
Mutant Lines

SUMMARYNucleotide-binding, leucine-rich repeat receptors (NLRs) are conserved cytosolic receptors that recognize pathogen effectors and trigger immunity in plants. Recent studies indicate that NLRs function in pairs. Rice resistance gene Pii has been known to confer resistance against rice blast pathogen Magnaporthe oryzae carrying AVR-Pii. Previously we reported isolation of Pii gene from the rice cultivar Hitomebore (Takagi et al. 2013). To further understand rice components required for Pii-mediated resistance, we screened 5,600 mutant lines of Hitomebore cultivar and identified two mutants that lost Pii resistance without any changes in Pii gene sequence. Application of MutMap-Gap, the whole genome sequencing-based method of mutation identification, to the two mutants revealed that they have mutations in another NLR gene located close to Pii. The F1 plants derived from a cross of the two mutants showed pii phenotype, demonstrating that the newly identified NLR gene is indeed a component of Pii resistance. We thus designate the previously isolated Pii gene as Pii-1 and the newly isolated NLR gene as Pii-2.

Marker Development for Rice Blast Resistance Gene Pi66(t) and Application in the USDA Rice Mini-Core Collection

Crop Science ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 1001-1008 ◽  
Author(s):  
Yan Liu ◽  
Yulin Jia ◽  
David Gealy ◽  
David M. Goad ◽  
Ana L. Caicedo ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Core Collection ◽  
Blast Resistance ◽  
Marker Development ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Mini Core Collection

scholarly journals Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates

2008 ◽  
Vol 22 (2) ◽  
pp. 289-299 ◽  
Author(s):  
Xin Xu ◽  
N. Hayashi ◽  
C. T. Wang ◽  
H. Kato ◽  
T. Fujimura ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Fine Mapping ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

scholarly journals Identification of novel alleles of the rice blast resistance gene Pi54

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Kumar Vasudevan ◽  
Wilhelm Gruissem ◽  
Navreet K. Bhullar
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Phosphorylation Site ◽  
Allelic Diversity ◽  
Resistance Breeding ◽  
Reference Sequence ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Base Pair Deletion

Abstract Rice blast is one of the most devastating rice diseases and continuous resistance breeding is required to control the disease. The rice blast resistance gene Pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India. We explored the allelic diversity of the Pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M. oryzae strains as well as against five unique strains. These genotypes are also annotated as rice blast resistant in the International Rice Genebank database. Sequence-based allele mining was used to amplify and clone the Pi54 allelic variants. Nine new alleles of Pi54 were identified based on the nucleotide sequence comparison to the Pi54 reference sequence as well as to already known Pi54 alleles. DNA sequence analysis of the newly identified Pi54 alleles revealed several single polymorphic sites, three double deletions and an eight base pair deletion. A SNP-rich region was found between a tyrosine kinase phosphorylation site and the nucleotide binding site (NBS) domain. Together, the newly identified Pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs.

Functional complementation of rice blast resistance gene Pi-k h (Pi54) conferring resistance to diverse strains of Magnaporthe oryzae

2011 ◽  
Vol 20 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Amit Kumar Rai ◽  
Satya Pal Kumar ◽  
Santosh Kumar Gupta ◽  
Naveen Gautam ◽  
Nagendera Kumar Singh ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Blast Resistance ◽  
Functional Complementation ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene
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