A bHLH transcription factor from Chenopodium glaucum confers drought tolerance to transgenic maize by positive regulation of morphological and physiological performances and stress-responsive genes’ expressions

2021 ◽  
Vol 41 (12) ◽  
Author(s):  
Haiju Zhao ◽  
Changhai Wang ◽  
Haiyan Lan
Keyword(s):  
Transcription Factor ◽  
Drought Tolerance ◽  
Transgenic Maize ◽  
Bhlh Transcription Factor ◽  
Positive Regulation ◽  
Stress Responsive Genes

Dominant Alleles of the Basic Helix-Loop-Helix Transcription Factor ATR2 Activate Stress-Responsive Genes in Arabidopsis

Genetics ◽  
2002 ◽  
Vol 161 (3) ◽  
pp. 1235-1246 ◽  
Author(s):  
Gromoslaw A Smolen ◽  
Laura Pawlowski ◽  
Sharon E Wilensky ◽  
Judith Bender
Keyword(s):  
Transcription Factor ◽  
Bhlh Transcription Factor ◽  
Wild Type ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Tryptophan Pathway ◽  
Dark Pigmentation ◽  
Stress Responsive Genes ◽  
Pathway Regulation ◽  
Myb Factor

AbstractMembers of the R/B basic helix-loop-helix (bHLH) family of plant transcription factors are involved in a variety of growth and differentiation processes. We isolated a dominant mutation in an R/B-related bHLH transcription factor in the course of studying Arabidopsis tryptophan pathway regulation. This mutant, atr2D, displayed increased expression of several tryptophan genes as well as a subset of other stress-responsive genes. The atr2D mutation creates an aspartate to asparagine change at a position that is highly conserved in R/B factors. Substitutions of other residues with uncharged side chains at this position also conferred dominant phenotypes. Moreover, overexpression of mutant atr2D, but not wild-type ATR2, conferred pleiotropic effects, including reduced size, dark pigmentation, and sterility. Therefore, atr2D is likely to be an altered-function allele that identifies a key regulatory site in the R/B factor coding sequence. Double-mutant analysis with atr1D, an overexpression allele of the ATR1 Myb factor previously isolated in tryptophan regulation screens, showed that atr2D and atr1D have additive effects on tryptophan regulation and are likely to act through distinct mechanisms to activate tryptophan genes. The dominant atr mutations thus provide tools for altering tryptophan metabolism in plants.

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