Technological Breakthrough for the Afforestation of Populus euphratica in the Mu Us Desert in China

The Mu Us Desert (MUD) is one of the four largest sandy lands in China. On 22 April 2020, the Shaanxi Forestry Bureau announced that the desertification land control rate in Yulin reached 93.24%, which means that the Mu Us Desert was about to “disappear” from the territory of Shaanxi. However, the problem of biological diversity, mostly for Pinus sylvestris and shrubs in the Mu Us Desert, remains serious. In order to consolidate the current forest conservation efforts, Populus euphratica has been considered an ideal candidate since the 1950s. However, the low survival rate and conservation rate of Populus euphratica in the MUD led us to perform further large-scale introduction for over 70 years. In this study, by using root control seedling technology, the survival and the conservation rate of Populus euphratica were increased to more than 90%. This study makes possible the introduction of Populus euphratica in the MUD, and the successful introduction of Populus euphratica will provide a new barrier for forest ecosystem stability in the desertification control project in the Yulin area.

Chemical characteristics of surface and phreatic water and their effects on the growth of Populus euphartica Oliv. in desert areas of Tarim National Nature Reserve, China

Chemical characteristics of surface water and phreatic water and their relationship with the growth of Populus euphratica Oliv. were explored. In the study area, hydrochemical types of the surface water were mainly SCaⅢ or ClNaⅢ (Based on the O. A. Arliekin's classification) and PH value of the surface water was below 8.0. The surface water from various sampling sites has a higher total hardness belonging to hard water and extremely hard water. The contents of sulfates and chlorides in the surface water in the protection zone ranged from 204.1 to 486.3 mg/l and from 70.9 to 239.9 mg/l, respectively. Phreatic water is mainly recharged by the surface water infiltration. In terms of water quality, TDS, total hardness and total alkalinity of phreatic water at W2 sampling point were 17.3, 9.3 and 4.7 times of surface water, respectively. Populus euphratica was able to grow normally in the environment of brackish water, where total hardness, total alkalinity, and TDS of overflowing river were in the range of 325.3~769.5, 142.6~290.3 and 782.2~1037.5 mg/l, respectively. The Populus euphratica in juvenile phase was a few, and its rejuvenation was confronted with difficulty. The present study can provide a reference for exploring the water quality characteristics of surface and phreatic water and their relationship with the growth of Populus euphratica. Bangladesh J. Bot. 50(3): 831-836, 2021 (September) Special

Genome-Wide Comprehensive Analysis of the GASA Gene Family in Populus

Gibberellic acid-stimulated Arabidopsis (GASA) proteins, as cysteine-rich peptides (CRPs), play roles in development and reproduction and biotic and abiotic stresses. Although the GASA gene family has been identified in plants, the knowledge about GASAs in Populus euphratica, the woody model plant for studying abiotic stress, remains limited. Here, we referenced the well-sequenced Populus trichocarpa genome, and identified the GASAs in the whole genome of P. euphratica and P. trichocarpa. 21 candidate genes in P. trichocarpa and 19 candidate genes in P. euphratica were identified and categorized into three subfamilies by phylogenetic analysis. Most GASAs with signal peptides were located extracellularly. The GASA genes in Populus have experienced multiple gene duplication events, especially in the subfamily A. The evolution of the subfamily A, with the largest number of members, can be attributed to whole-genome duplication (WGD) and tandem duplication (TD). Collinearity analysis showed that WGD genes played a leading role in the evolution of GASA genes subfamily B. The expression patterns of P. trichocarpa and P. euphratica were investigated using the PlantGenIE database and the real-time quantitative PCR (qRT-PCR), respectively. GASA genes in P. trichocarpa and P. euphratica were mainly expressed in young tissues and organs, and almost rarely expressed in mature leaves. GASA genes in P. euphratica leaves were also widely involved in hormone responses and drought stress responses. GUS activity assay showed that PeuGASA15 was widely present in various organs of the plant, especially in vascular bundles, and was induced by auxin and inhibited by mannitol dramatically. In summary, this present study provides a theoretical foundation for further research on the function of GASA genes in P. euphratica.

Frequent ploidy changes in Salicaceae indicates widespread sharing of the salicoid whole genome duplication by the relatives of Populus L. and Salix L.

Backgrounds Populus and Salix belong to Salicaceae and are used as models to investigate woody plant physiology. The variation of karyotype and nuclear DNA content can partly reflect the evolutionary history of the whole genome, and can provide critical information for understanding, predicting, and potentially ameliorating the woody plant traits. Therefore, it is essential to study the chromosome number (CN) and genome size in detail to provide information for revealing the evolutionary process of Salicaceae. Results In this study, we report the somatic CNs of seventeen species from eight genera in Salicaceae. Of these, CNs for twelve species and for five genera are reported for the first time. Among the three subfamilies of Salicaceae, the available data indicate CN in Samydoideae is n = 21, 22, 42. The only two genera, Dianyuea and Scyphostegia, in Scyphostegioideae respectively have n = 9 and 18. In Salicoideae, Populus, Salix and five genera closely related to them (Bennettiodendron, Idesia, Carrierea, Poliothyrsis, Itoa) are based on relatively high CNs from n = 19, 20, 21, 22 to n = 95 in Salix. However, the other genera of Salicoideae are mainly based on relatively low CNs of n = 9, 10, 11. The genome sizes of 35 taxa belonging to 14 genera of Salicaceae were estimated. Of these, the genome sizes of 12 genera and all taxa except Populus euphratica are first reported. Except for Dianyuea, Idesia and Bennettiodendron, all examined species have relatively small genome sizes of less than 1 pg, although polyploidization exists. Conclusions The variation of CN and genome size across Salicaceae indicates frequent ploidy changes and a widespread sharing of the salicoid whole genome duplication (WGD) by the relatives of Populus and Salix. The shrinkage of genome size after WGD indicates massive loss of genomic components. The phylogenetic asymmetry in clade of Populus, Salix, and their close relatives suggests that there is a lag-time for the subsequent radiations after the salicoid WGD event. Our results provide useful data for studying the evolutionary events of Salicaceae.

Populus euphratica Phenology and Its Response to Climate Change in the Upper Tarim River Basin, NW China

Quantifying the phenological variations of Populus euphratica Olivier (P. euphratica) resulting from climate change is vital for desert ecosystems. There has previously been great progress in the influence of climate change on vegetation phenology, but knowledge of the variations in P. euphratica phenology is lacking in extremely arid areas. In this study, a modified method was proposed to explore P. euphratica phenology and its response to climate change using 18-year Global Land Surface Satellite (GLASS) leaf area index (LAI) time series data (2000–2017) in the upper Tarim River basin. The start of the growing season (SOS), length of the growing season (LOS), and end of the growing season (EOS) were obtained with the dynamic threshold method from the reconstructed growth time series curve by using the Savitzky–Golay filtering method. The grey relational analysis (GRA) method was utilized to analyze the influence between the phenology and the key climatic periods and factors. Importantly, we also revealed the positive and negative effects between interannual climate factors and P. euphratica phenology using the canonical correlation analysis (CCA) method, and the interaction between the SOS in spring and EOS in autumn. The results revealed that trends of P. euphratica phenology (i.e., SOS, EOS, and LOS) were not significant during the period from 2000–2017. The spring temperature and sunshine duration (SD) controlled the SOS, and the EOS was mainly affected by the temperature and SD from June–November, although the impacts of average relative humidity (RH) and precipitation (PR) on the SOS and EOS cannot be overlooked. Global warming may lead to SOS advance and EOS delay, and the increase in SD and PR may lead to earlier SOS and later EOS. Runoff was found to be a more key factor for controlling P. euphratica phenology than PR in this region.

Estimation of Populuseuphratica Forest Leaf Litterfall and Time Variation of Nutrient in Leaf Litter during Decomposition along the Main Channel of the Tarim River, China

Accurate determination of annual leaf litter amount constitutes the basis of scientific leaf litter nutrient release assessment. In this study, we tried to establish an equation between leaf litter amount and relevant tree characteristics of Populus euphratica (P. euphratica) tree on an individual scale, and to find the leaf litter nutrient content variation within 760 d incubation experiment in the main channel of the Tarim River, China. Results showed that there was no proper equation between leaf litter amount and tree height or diameter at breast height. There was great difference in leaf litter amount on an individual scale. The mean annual leaf litter amount per tree was 10.2, 14.83 kg/y obtained by field survey and the equation between annual leaf litter amount and canopy area on an individual scale, respectively. Leaf litter mass changed over incubation time and exhibited three main phases: an initial slow decomposition phase (0–173 d) with mass loss; a rapid mass loss phase (173–290 d); and a second rapid mass loss phase (470–560 d). Overall, carbon (C) and potassium (K) content decreased, and nitrogen (N) and phosphorus (P) content increased in a fluctuating manner over time in the P. euphratica leaf litter.

Populus euphratica Apyrases Increase Drought Tolerance by Modulating Stomatal Aperture in Arabidopsis

Stomatal regulation is crucial to reduce water consumption under drought conditions. Extracellular ATP (eATP) serves as a signaling agent in stomatal regulation; however, it is less known whether the eATP mediation of stomatal aperture is linked to apyrases (APYs), the principal enzymes that control the concentration of eATP. To clarify the role of APYs in stomatal control, PeAPY1 and PeAPY2 were isolated from Populus euphratica and transferred into Arabidopsis. Compared with the wild-type Arabidopsis and loss-of-function mutants (Atapy1 and Atapy2), PeAPY1- and PeAPY2-transgenic plants decreased stomatal aperture under mannitol treatment (200 mM, 2 h) and reduced water loss during air exposure (90 min). The role of apyrase in stomatal regulation resulted from its control in eATP-regulated stomatal movements and increased stomatal sensitivity to ABA. The bi-phasic dose-responses to applied nucleotides, i.e., the low ATP (0.3–1.0 mM)-promoted opening and high ATP (>2.0 mM)-promoted closure, were both restricted by P. euphratica apyrases. It is noteworthy that eATP at a low concentration (0.3 mM) counteracted ABA action in the regulation of stomatal aperture, while overexpression of PeAPY1 or PeAPY2 effectively diminished eATP promotion in opening, and consequently enhanced ABA action in closure. We postulate a speculative model of apyrase signaling in eATP- and ABA-regulated stomatal movements under drought.