Cloning and expression of a cellulase gene in the silkworm, Bombyx mori by improved Bac-to-Bac/BmNPV baculovirus expression system

2010 ◽  
Vol 37 (8) ◽  
pp. 3721-3728 ◽  
Author(s):  
Xing-hua Li ◽  
Dan Wang ◽  
Fang Zhou ◽  
Hua-jun Yang ◽  
Roy Bhaskar ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Cloning And Expression ◽  
Cellulase Gene ◽  
Baculovirus Expression ◽  
Silkworm Bombyx Mori

scholarly journals Expression of Glycosylated Mucin–like Domain Using Baculovirus Expression System in Silkworm, Bombyx mori

10.5109/22053 ◽  
2012 ◽  
Vol 57 (1) ◽  
pp. 83-86
Author(s):  
Yudai Nagata ◽  
Kosuke Sakashita ◽  
Shigeo Imanishi ◽  
Jae Man Lee ◽  
Takahiro Kusakabe
Keyword(s):  
Bombyx Mori ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Baculovirus Expression ◽  
Silkworm Bombyx Mori

scholarly journals Cloning and expression of hepatitis E virus ORF2 as an immunogen protein in baculovirus expression system

2018 ◽  
Vol 5 (1) ◽  
pp. 23-26
Author(s):  
SA Sadeghi ◽  
M Shahanaghi ◽  
MR Aghasadeghi ◽  
F Motevalli ◽  
MR Amiran ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Hepatitis E ◽  
Cloning And Expression ◽  
Baculovirus Expression

Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system

2006 ◽  
Vol 71 (2) ◽  
pp. 192-199 ◽  
Author(s):  
Yungen Miao ◽  
Yuansong Zhang ◽  
Koichi Nakagaki ◽  
Tianfu Zhao ◽  
Aichun Zhao ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Cell Line ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Silk Protein ◽  
Baculovirus Expression

PRODUCTION OF RECOMBINANT OVINE INTERFERON TAU USING A BOMBYX MORI NUCLEAR POLYHEDROSIS BACULOVIRUS EXPRESSION SYSTEM

2002 ◽  
Vol 13 (1) ◽  
pp. 147-158 ◽  
Author(s):  
S. J. Mann ◽  
G. E. Lamming ◽  
P. A. Fisher ◽  
S. Maeda ◽  
H. J. Stewart ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Interferon Tau ◽  
Baculovirus Expression ◽  
Nuclear Polyhedrosis

scholarly journals Expression of functional G protein beta gamma dimers of defined subunit composition using a baculovirus expression system.

1992 ◽  
Vol 267 (19) ◽  
pp. 13123-13126 ◽  
Author(s):  
S.G. Graber ◽  
R.A. Figler ◽  
V.K. Kalman-Maltese ◽  
J.D. Robishaw ◽  
J.C. Garrison
Keyword(s):  
G Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Subunit Composition ◽  
Baculovirus Expression

scholarly journals Production, processing and partial purification of functional G protein βγ subunits in baculovirus-infected insect cells

1992 ◽  
Vol 286 (3) ◽  
pp. 677-680 ◽  
Author(s):  
J D Robishaw ◽  
V K Kalman ◽  
K L Proulx
Keyword(s):  
G Protein ◽  
G Proteins ◽  
Insect Cells ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Partial Purification ◽  
Baculovirus Expression ◽  
Gamma Subunits ◽  
Infected Insect ◽  
Beta 2

As a result of the inability to resolve the heterogeneous mixture of G protein beta gamma subunits present in tissues, it has not been possible to compare different beta gamma subunits of the G proteins in terms of their proposed roles in receptor-effector coupling. This study was undertaken to establish the utility of the baculovirus expression system in producing homogeneous beta gamma subunits of defined composition for the comparative analysis of these subunits in reconstitution systems. In this study we report the expression, and appropriate post-translational processing, of recombinant beta 2, gamma 2 and gamma 3 subunits. In addition, we show that the recombinant beta gamma subunits can be readily purified, and can functionally interact with the alpha subunits of the G proteins.

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