scholarly journals Molecular genotypic diversity of populations of brinjal shoot and fruit borer, Leucinodes orbonalis and development of SCAR marker for pesticide resistance

2021 ◽  
Author(s):  
Palraju Murali ◽  
Karuppiah Hilda ◽  
Muthusamy Ramakrishnan ◽  
Arumugam Ganesh ◽  
Sreeramulu Bhuvaragavan ◽  
...  
Keyword(s):  
Scar Marker ◽  
Genotypic Diversity ◽  
Pesticide Resistance ◽  
Leucinodes Orbonalis ◽  
Fruit Borer

scholarly journals Molecular Genotypic Diversity of Populations of Brinjal Shoot and Fruit Borer, Leucinodes Orbonalis and Development of SCAR Marker for Pesticide Resistance

2021 ◽  
Author(s):  
Palraju Murali ◽  
Karuppiah Hilda ◽  
Muthusamy Ramakrishnan ◽  
Arumugam Ganesh ◽  
Sreeramulu Bhuvaragavan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Tamil Nadu ◽  
Scar Marker ◽  
Random Amplified Polymorphic Dna ◽  
Pesticide Resistance ◽  
Specific Marker ◽  
Leucinodes Orbonalis ◽  
Fruit Borer ◽  
Different Populations

Abstract The brinjal shoot and fruit borer, Leucinodes orbonalis is a destructive pest of Solanum melongena. The control of L. orbonalis with extensive application of synthetic chemical insecticides resulted in the development of resistance with known genetic heterogeneity among populations. Understanding the genetic diversity of their populations is important in developing strategies for their management. The present investigation was performed to characterize populations of L. orbonalis for their genetic diversity in the entire region of Tamil Nadu, South India using random amplified polymorphic DNA (RAPD) primers as a tool of the molecular marker. Among sixty random 10-mer primers, only ten primers generated reproducible and scorable banding profile. Among the ten different random primers, the primers namely OPG 7, OPG 8, OPS 2 and OPS 7 generated the highest genetic variation with over 80% genetic polymorphism. Phylogram analysis produced 18 clusters with 8 major and 10 minor clusters. Cluster analysis, statistical fitness, population structure and analysis of molecular variance confirmed the significant genetic variation among different populations. A trait specific marker obtained through RAPD was cloned, sequenced and used to develop a stable diagnostic SCAR marker for DNA fingerprinting to distinguish the populations. Amplification of this locus in the samples of 20 different populations indicated recognition of the trait for pesticide resistance in 12 populations. The results suggest that the biochemical nature of host plant varieties of this insect pest and variation in the application of different insecticides are essential contributing factors for the genotypic variations observed among populations of L. orbonalis.

scholarly journals Molecular genotypic diversity of populations of brinjal shoot and fruit borer, Leucinodes orbonalis and development of SCAR marker for pesticide resistance

2021 ◽  
Author(s):  
Palraju Murali ◽  
Karuppiah Hilda ◽  
Muthusamy Ramakrishnan ◽  
Arumugam Ganesh ◽  
Sreeramulu Bhuvaragavan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Tamil Nadu ◽  
Scar Marker ◽  
Random Amplified Polymorphic Dna ◽  
Pesticide Resistance ◽  
Specific Marker ◽  
Leucinodes Orbonalis ◽  
Fruit Borer ◽  
Different Populations

Abstract The brinjal shoot and fruit borer, Leucinodes orbonalis is a destructive pest of Solanum melongena. The control of L. orbonalis with extensive application of synthetic chemical insecticides resulted in the development of resistance with known genetic heterogeneity among populations. Understanding the genetic diversity of their populations is important in developing strategies for their management. The present investigation was performed to characterize populations of L. orbonalis for their genetic diversity in the entire region of Tamil Nadu, South India using random amplified polymorphic DNA (RAPD) primers as a tool of the molecular marker. Among sixty random 10-mer primers, only ten primers generated reproducible and scorable banding profile. Among the ten different random primers, the primers namely OPG 7, OPG 8, OPS 2 and OPS 7 generated the highest genetic variation with over 80% genetic polymorphism. Phylogram analysis produced 18 clusters with 8 major and 10 minor clusters. Cluster analysis, statistical fitness, population structure and analysis of molecular variance confirmed the significant genetic variation among different populations. A trait specific marker obtained through RAPD was cloned, sequenced and used to develop a stable diagnostic SCAR marker for DNA fingerprinting to distinguish the populations. Amplification of this locus in the samples of 20 different populations indicated recognition of the trait for pesticide resistance in 12 populations. The results suggest that the biochemical nature of host plant varieties of this insect pest and variation in the application of different insecticides are essential contributing factors for the genotypic variations observed among populations of L. orbonalis.

Studies on the Seasonal Influence on the Occurrence of Shoot and Fruit Borer (Leucinodes Orbonalis Guenee) in Brinjal (Solanum Melongena L.) Var. Kkm-1

2011 ◽  
Vol 4 (8) ◽  
pp. 1-3
Author(s):  
S. AYYANAR S. AYYANAR ◽  
◽  
Dr. M. A. K. Pillai Dr. M. A. K. Pillai ◽  
Dr. N. Murugesan Dr. N. Murugesan
Keyword(s):  
Solanum Melongena ◽  
Seasonal Influence ◽  
Leucinodes Orbonalis ◽  
Fruit Borer

scholarly journals Appraisal of resistant genotypes against brinjal shoot and fruit borer (BSFB), Leucinodes orbonalis, Guenee

2018 ◽  
Vol 16 (2) ◽  
pp. 227-231
Author(s):  
Hafija Sultana ◽  
Md Aabdul Mannan ◽  
Md Mostofa Kamal ◽  
Khan Golam Quddus ◽  
Shimul Das
Keyword(s):  
Pot Experiment ◽  
Leucinodes Orbonalis ◽  
Fruit Borer ◽  
Three Stages ◽  
Fruit Damage ◽  
Shoot Damage ◽  
Resistant Genotypes

The main purpose of this study was to find out the best variety of brinjal having resistance to BSFB. A pot experiment was carried out at the germplasm centre, Agrotechnology Discipline, Khulna University, Khulna during February 2014 to August 2014. The experiment was laid out in CRD using thirteen varieties with five replications. The varieties were V1=BARI begun-1, V2=BARI begun-4, V3=BARI begun-5, V4=BARI begun-6, V5=BARI begun-7, V6=BARI begun-8, V7=BARI begun-9, V8=BARI begun-10, V9=Makra, V10=Muktokashi, V11=Lalita, V12=Hazra, V13=Chaga. Data were collected on total number of shoot, total number of infested shoot, percentage of shoot damage, percentage of shoot infestation reduction, numbers of larvae per infested shoot, total number of fruit, percentage of fruit damage, percentage of fruit infestation reduction, numbers of larvae per infested fruit. Results have shown that the minimum shoot infestation was found in BARI begun-6 (29.60%, 32.40%, 29.86% and 29.38%, respectively at four different stages of eggplant). Percent of fruit infestation was minimum in V4 (BARI begun-6) (25.16%, 27.42% and 25.40% at three stages respectively).J. Bangladesh Agril. Univ. 16(2): 227-231, August 2018

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