Persistence and biological activity in soil of the insecticidal proteins from Bacillus thuringiensis, especially from transgenic plants

2005 ◽  
Vol 266 (1-2) ◽  
pp. 77-89 ◽  
Author(s):  
G. Stotzky
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis ◽  
Transgenic Plants ◽  
Insecticidal Proteins

scholarly journals Selection and Characterization of Bacillus thuringiensis Strains with Biological Activity against Lycoriella ingenua

2018 ◽  
Vol 63 (2) ◽  
pp. 293-302
Author(s):  
You Kyoung LEE ◽  
Na Young JIN ◽  
Hee Ji KIM ◽  
Yong Man YOUN ◽  
Chisa YASUNAGA–AOKI ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis

Biological Activity of Bacillus thuringiensis (Bacillales: Bacillaceae) in Anastrepha fraterculus (Diptera: Tephritidae)

2018 ◽  
Vol 111 (3) ◽  
pp. 1486-1489 ◽  
Author(s):  
Liliane Nachtigall Martins ◽  
Ana Paula de Souza Stori de Lara ◽  
Márcio Soares Ferreira ◽  
Adrise Medeiros Nunes ◽  
Daniel Bernardi ◽  
...  
Keyword(s):  
Biological Activity ◽  
Bacillus Thuringiensis ◽  
Anastrepha Fraterculus

scholarly journals Bacillus thuringiensis Vip1 Functions as a Receptor of Vip2 Toxin for Binary Insecticidal Activity against Holotrichia parallela

Toxins ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 440 ◽  
Author(s):  
Jianxun Geng ◽  
Jian Jiang ◽  
Changlong Shu ◽  
Zeyu Wang ◽  
Fuping Song ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Affinity Chromatography ◽  
Insecticidal Activity ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Lethal Concentration ◽  
Insecticidal Proteins ◽  
Vegetative Phase ◽  
Holotrichia Parallela ◽  
Weak Binding

Bacillus thuringiensis is a well-known entomopathogenic bacterium that produces vegetative insecticidal proteins (Vips, including Vip1, Vip2, Vip3, and Vip4) during the vegetative phase. Here, we purified Vip1 and Vip2 from B. thuringiensis and characterized the insecticidal effects of these protoxins. Bioassay results showed that a 1:1 mixture of Vip1Ad and Vip2Ag, purified by ion-affinity chromatography independently, exhibited insecticidal activity against Holotrichia parallela larvae, with a 50% lethal concentration value of 2.33 μg/g soil. The brush border membrane (BBM) in the midgut of H. parallela larvae was destroyed after feeding the Vip1Ad and Vip2Ag mixture. Vacuolization of the cytoplasm and slight destruction of BBM were detected with Vip2Ag alone, but not with Vip1Ad alone. Notably, Vip1Ad bound to BBM vesicles (BBMVs) strongly, whereas Vip2Ag showed weak binding; however, binding of Vip2Ag to BBMV was increased when Vip1Ad was added. Ligand blotting showed that Vip2Ag did not bind to Vip1Ad but bound to Vip1Ad-t (Vip1Ad was activated by trypsin), suggesting the activation of Vip1Ad was important for their binary toxicity. Thus, our findings suggested that Vip1Ad may facilitate the binding of Vip2Ag to BBMVs, providing a basis for studies of the insecticidal mechanisms of Vip1Ad and Vip2Ag.

Insecticidal proteins from Bacillus thuringiensis protect corn from corn rootworms

10.1038/90282 ◽  
2001 ◽  
Vol 19 (7) ◽  
pp. 668-672 ◽  
Author(s):  
Daniel J. Moellenbeck ◽  
Melvin L. Peters ◽  
James W. Bing ◽  
James R. Rouse ◽  
Laura S. Higgins ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Proteins ◽  
Corn Rootworms
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