A comparison of genetic stability in tea [Camellia sinensis (L.) Kuntze] plantlets derived from callus with plantlets from long-term in vitro propagation

2019 ◽  
Vol 138 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Lidiia Samarina ◽  
Maya Gvasaliya ◽  
Natalia Koninskaya ◽  
Ruslan Rakhmangulov ◽  
Alexander Efremov ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Genetic Stability ◽  
In Vitro Propagation

In vitro propagation and assessment of genetic stability of micropropagated Samanea saman (rain tree) using microsatellite markers

2013 ◽  
Vol 35 (8) ◽  
pp. 2467-2474 ◽  
Author(s):  
Sampath Kasthurirengan ◽  
Lifen Xie ◽  
Chun Hong Li ◽  
Yok King Fong ◽  
Yan Hong
Keyword(s):  
Microsatellite Markers ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Samanea Saman

In vitro propagation of endangered Mammillaria genus (Cactaceae) species and genetic stability assessment using SSR markers

2018 ◽  
Vol 54 (5) ◽  
pp. 518-529 ◽  
Author(s):  
Jesús Omar Lázaro-Castellanos ◽  
Martín Mata-Rosas ◽  
Dolores González ◽  
Salvador Arias ◽  
Frédérique Reverchon
Keyword(s):  
Ssr Markers ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Stability Assessment

In vitro propagation of Plum (Prunus salicina) cv. ‘Santa Rosa’ and assessment of genetic stability using RAPD markers

2018 ◽  
Vol 23 (1) ◽  
pp. 161-168 ◽  
Author(s):  
Manisha Thakur ◽  
Madhvi Soni ◽  
Dharam Paul Sharma ◽  
Manu Vivek ◽  
Vishal Sharma
Keyword(s):  
Genetic Stability ◽  
In Vitro Propagation ◽  
Rapd Markers ◽  
Prunus Salicina

scholarly journals ITGA6+ Human Testicular Cell Populations Acquire a Mesenchymal Rather than Germ Cell Transcriptional Signature during Long-Term Culture

2020 ◽  
Vol 21 (21) ◽  
pp. 8269
Author(s):  
Robert B. Struijk ◽  
Callista L. Mulder ◽  
Saskia K. M. van Daalen ◽  
Cindy M. de Winter-Korver ◽  
Aldo Jongejan ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Sorting ◽  
In Vitro Propagation ◽  
Cell Type ◽  
Testicular Cell ◽  
Testicular Cells ◽  
Cell Type Composition ◽  
Type Composition

Autologous spermatogonial stem cell transplantation is an experimental technique aimed at restoring fertility in infertile men. Although effective in animal models, in vitro propagation of human spermatogonia prior to transplantation has proven to be difficult. A major limiting factor is endogenous somatic testicular cell overgrowth during long-term culture. This makes the culture both inefficient and necessitates highly specific cell sorting strategies in order to enrich cultured germ cell fractions prior to transplantation. Here, we employed RNA-Seq to determine cell type composition in sorted integrin alpha-6 (ITGA6+) primary human testicular cells (n = 4 donors) cultured for up to two months, using differential gene expression and cell deconvolution analyses. Our data and analyses reveal that long-term cultured ITGA6+ testicular cells are composed mainly of cells expressing markers of peritubular myoid cells, (progenitor) Leydig cells, fibroblasts and mesenchymal stromal cells and only a limited percentage of spermatogonial cells as compared to their uncultured counterparts. These findings provide valuable insights into the cell type composition of cultured human ITGA6+ testicular cells during in vitro propagation and may serve as a basis for optimizing future cell sorting strategies as well as optimizing the current human testicular cell culture system for clinical use.

In vitro propagation, genetic stability, and secondary metabolite analysis of wild lavender (Lavandula coronopifolia Poir.)

2017 ◽  
Vol 58 (4) ◽  
pp. 393-405 ◽  
Author(s):  
Wesam Al Khateeb ◽  
Razan Kanaan ◽  
Tamam El-Elimat ◽  
Muhammad Alu’datt ◽  
Jamil Lahham ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Metabolite Analysis
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