scholarly journals In vitro propagation and assessment of genetic stability of acclimated plantlets of Cornus alba L. using RAPD and ISSR markers

2016 ◽  
Vol 52 (4) ◽  
pp. 379-390 ◽  
Author(s):  
Agnieszka Ilczuk ◽  
Ewelina Jacygrad
Keyword(s):  
Genetic Stability ◽  
In Vitro Propagation ◽  
Issr Markers ◽  
Cornus Alba ◽  
Rapd And Issr

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC

Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Regenerated Plants ◽  
Rapd And Issr

scholarly journals In Vitro Propagation and Detection of Somaclonal Variation in Phalaenopsis gigantea as Affected by Chitosan and Thidiazuron Combinations

HortScience ◽  
2014 ◽  
Vol 49 (1) ◽  
pp. 82-88 ◽  
Author(s):  
Samira Samarfard ◽  
Mihdzar A. Kadir ◽  
Saleh B. Kadzimin ◽  
Halimi M. Saud ◽  
Seyed Ali Ravanfar ◽  
...  
Keyword(s):  
Genetic Stability ◽  
In Vitro Propagation ◽  
Genetic Fidelity ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Mother Plant ◽  
Apical Region ◽  
Sequence Repeat ◽  
In Vitro Methods

Protocorm-like bodies (PLBs) multiplication is one of the most preferable in vitro methods to increase the number of orchids that produce very few seeds or seeds that are not able to germinate. In the present study the effects of chitosan and thidiazuron (TDZ) combinations on multiplication, differentiation, and genetic stability of Phalaenopsis gigantea PLBs were investigated using different media. Initial PLBs were cultured in solid New Dogashima (ND) medium and Vacin and Went (VW) medium supplemented with different concentrations of chitosan (0, 5, 10, 15, 20, and 25 mg·L–1) and TDZ (0, 0.1, and 0.5 mg·L–1). The highest mean number of PLBs (353 PLBs) was observed in ND medium with 10 mg·L–1 chitosan and 0.1 mg·L–1 TDZ combination after 20 weeks of culture. Some PLBs differentiated into mature PLBs with a profusion of leaves on the apical region, and tiny plantlets started to develop after 10 weeks of culture. The highest mean number of shoots was observed in VW supplemented with 10 mg·L–1 chitosan and 0.5 mg·L–1 TDZ (16 shoots). Intersimple sequence repeat (ISSR) markers were used to determine the genetic fidelity among mother plant and PLBs obtained from each subculture stage of solid ND medium supplemented with 10 mg·L–1 chitosan and 0.1 mg·L–1 TDZ (the optimal treatment for PLB proliferation). Dissimilarity of 5% occurred between the mother plant and PLBs obtained after 16 weeks of culture. The range in the similarity coefficient varied from 0.80 to 1.0, and only 20% dissimilarity occurred between mother plant and PLBs after 20 weeks of culture.

In vitro propagation and assessment of genetic stability of micropropagated Samanea saman (rain tree) using microsatellite markers

2013 ◽  
Vol 35 (8) ◽  
pp. 2467-2474 ◽  
Author(s):  
Sampath Kasthurirengan ◽  
Lifen Xie ◽  
Chun Hong Li ◽  
Yok King Fong ◽  
Yan Hong
Keyword(s):  
Microsatellite Markers ◽  
Genetic Stability ◽  
In Vitro Propagation ◽  
Samanea Saman

Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR markers

2011 ◽  
Vol 55 (1) ◽  
pp. 16-20 ◽  
Author(s):  
S. Mohanty ◽  
M. K. Panda ◽  
S. Sahoo ◽  
S. Nayak
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Rapd And Issr
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