Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment

2007 ◽  
Vol 16 (5) ◽  
pp. 587-597 ◽  
Author(s):  
Linda Avesani ◽  
Giampiero Marconi ◽  
Francesca Morandini ◽  
Emidio Albertini ◽  
Matteo Bruschetta ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Potato Virus ◽  
Potato Virus X ◽  
Expression Vectors ◽  
Insert Size

scholarly journals Peptide display on Potato virus X: molecular features of the coat protein-fused peptide affecting cell-to-cell and phloem movement of chimeric virus particles

2006 ◽  
Vol 87 (10) ◽  
pp. 3103-3112 ◽  
Author(s):  
Chiara Lico ◽  
Floriana Capuano ◽  
Giovanni Renzone ◽  
Marcello Donini ◽  
Carla Marusic ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus X ◽  
Structural Features ◽  
Plant Viruses ◽  
Chimeric Virus ◽  
Expression Vectors ◽  
In Planta ◽  
Virus Particles ◽  
Molecular Features

The potexvirus Potato virus X (PVX) can be modified genetically to generate chimeric virus particles (CVPs) carrying heterologous peptides fused to coat protein (CP) subunits. A spontaneous PVX mutant expressing a truncated, but functional, form of the CP has been isolated. With the aim of exploiting this virus to display peptides useful for vaccine formulations, two novel viral expression vectors based on pPVX201 (bearing the wild-type PVX genome) were constructed encoding the truncated CP. Both vectors were able to produce infectious virus particles in planta and were used to insert a panel of sequences encoding peptides of biopharmaceutical interest as N-terminal fusions to the truncated cp gene. The analysis of infection progression induced by the different constructs enabled identification of two important structural features of the fused peptide, namely tryptophan content and isoelectric point, critically affecting the formation of PVX CVPs and virus movement through the plant. These results are discussed in view of the rising interest in engineered plant viruses for development of peptide-based epitope vaccines.

Potato virus X-based expression vectors are stabilized for long-term production of proteins and larger inserts

2014 ◽  
Vol 9 (11) ◽  
pp. 1369-1379 ◽  
Author(s):  
Christina Dickmeis ◽  
Rainer Fischer ◽  
Ulrich Commandeur
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Expression Vectors

scholarly journals Stability of recombinant plant viruses containing genes of unrelated plant viruses

2007 ◽  
Vol 88 (4) ◽  
pp. 1347-1355 ◽  
Author(s):  
Bong-Nam Chung ◽  
Tomas Canto ◽  
Peter Palukaitis
Keyword(s):  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Potato Virus ◽  
Tobacco Rattle Virus ◽  
Resistance Mechanism ◽  
Potato Virus X ◽  
Plant Viruses ◽  
Hybrid Plant ◽  
Expression Vectors ◽  
Post Inoculation

The stability of hybrid plant viruses that might arise by recombination in transgenic plants was examined using hybrid viruses derived from the viral expression vectors potato virus X (PVX) and tobacco rattle virus (TRV). The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was introduced into a vector derived from TRV RNA2 to generate TRV-NIb. All three viruses were unstable and most of the progeny viruses had lost the inserted sequences between 2 and 4 weeks post-inoculation. There was some variation in the rate of loss of part or all of the inserted sequence and the number of plants containing the deleted viruses, depending on the sequence, the host (Nicotiana tabacum vs Nicotiana benthamiana) or the vector, although none of these factors was associated consistently with the preferential loss of the inserted sequences. PVX-NIb was unable to accumulate in NIb-transgenic tobacco resistant to infection by PVY and also showed loss of the NIb insert from PVX-NIb in some NIb-transgenic tobacco plants susceptible to infection by PVY. These data indicate that such hybrid viruses, formed in resistant transgenic plants from a transgene and an unrelated virus, would be at a selective disadvantage, first by being targeted by the resistance mechanism and second by not being competitive with the parental virus.

Potato virus X amplicon-mediated silencing of nuclear genes

1999 ◽  
Vol 20 (3) ◽  
pp. 357-362 ◽  
Author(s):  
Susan M. Angell ◽  
David C. Baulcombe
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Nuclear Genes

Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs

Acta Naturae ◽  
2011 ◽  
Vol 3 (3) ◽  
pp. 40-46 ◽  
Author(s):  
M V Arkhipenko ◽  
E K Petrova ◽  
N A Nikitin ◽  
A D Protopopova ◽  
E V Dubrovin ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus X ◽  
Viral Rnas ◽  
Virus Like Particles ◽  
Artificial Virus

STUDY ОF SOME MOLECULAR-GENETIK PROPERTIES OF THE POTATO VIRUS X

2020 ◽  
Vol 4 (2) ◽  
pp. 4-11
Author(s):  
Vokhid Fayziev ◽  
◽  
Dilfuza Javlieva ◽  
Umida Jurayeva ◽  
Zarifa Kadirova ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus X

scholarly journals Transcriptional Regulatory Networks Associate with Early Stages of Potato Virus X Infection of Solanum tuberosum

2021 ◽  
Vol 22 (6) ◽  
pp. 2837
Author(s):  
Venura Herath ◽  
Jeanmarie Verchot
Keyword(s):  
Potato Virus ◽  
Regulatory Networks ◽  
Potato Virus X ◽  
Early Response ◽  
Pathogen Resistance ◽  
Transcriptional Regulatory Networks ◽  
Unfolded Protein ◽  
Molecular Pattern ◽  
Transcriptional Regulatory ◽  
Protein Response

Potato virus X (PVX) belongs to genus Potexvirus. This study characterizes the cellular transcriptome responses to PVX infection in Russet potato at 2 and 3 days post infection (dpi). Among the 1242 differentially expressed genes (DEGs), 268 genes were upregulated, and 37 genes were downregulated at 2 dpi while 677 genes were upregulated, and 265 genes were downregulated at 3 dpi. DEGs related to signal transduction, stress response, and redox processes. Key stress related transcription factors were identified. Twenty-five pathogen resistance gene analogs linked to effector triggered immunity or pathogen-associated molecular pattern (PAMP)-triggered immunity were identified. Comparative analysis with Arabidopsis unfolded protein response (UPR) induced DEGs revealed genes associated with UPR and plasmodesmata transport that are likely needed to establish infection. In conclusion, this study provides an insight on major transcriptional regulatory networked involved in early response to PVX infection and establishment.

scholarly journals Perturbation of nuclear‐cytosolic shuttling of Rx1 compromises extreme resistance and translational arrest of potato virus X transcripts

2021 ◽  
Author(s):  
Manon MS Richard ◽  
Marijn Knip ◽  
Joëlle Schachtschabel ◽  
Machiel S Beijaert ◽  
Frank LW Takken
Keyword(s):  
Potato Virus ◽  
Potato Virus X ◽  
Extreme Resistance ◽  
Translational Arrest

Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity

2008 ◽  
Vol 137 (1) ◽  
pp. 16-23 ◽  
Author(s):  
Natalia Andrea Módena ◽  
Alicia Mercedes Zelada ◽  
Florencia Conte ◽  
Alejandro Mentaberry
Keyword(s):  
Nicotiana Tabacum ◽  
Potato Virus ◽  
Movement Protein ◽  
Potato Virus X
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