scholarly journals Peptide display on Potato virus X: molecular features of the coat protein-fused peptide affecting cell-to-cell and phloem movement of chimeric virus particles

2006 ◽  
Vol 87 (10) ◽  
pp. 3103-3112 ◽  
Author(s):  
Chiara Lico ◽  
Floriana Capuano ◽  
Giovanni Renzone ◽  
Marcello Donini ◽  
Carla Marusic ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus X ◽  
Structural Features ◽  
Plant Viruses ◽  
Chimeric Virus ◽  
Expression Vectors ◽  
In Planta ◽  
Virus Particles ◽  
Molecular Features

The potexvirus Potato virus X (PVX) can be modified genetically to generate chimeric virus particles (CVPs) carrying heterologous peptides fused to coat protein (CP) subunits. A spontaneous PVX mutant expressing a truncated, but functional, form of the CP has been isolated. With the aim of exploiting this virus to display peptides useful for vaccine formulations, two novel viral expression vectors based on pPVX201 (bearing the wild-type PVX genome) were constructed encoding the truncated CP. Both vectors were able to produce infectious virus particles in planta and were used to insert a panel of sequences encoding peptides of biopharmaceutical interest as N-terminal fusions to the truncated cp gene. The analysis of infection progression induced by the different constructs enabled identification of two important structural features of the fused peptide, namely tryptophan content and isoelectric point, critically affecting the formation of PVX CVPs and virus movement through the plant. These results are discussed in view of the rising interest in engineered plant viruses for development of peptide-based epitope vaccines.

Comparative structural stability of subunits of the potato virus X coat protein in solution and in virus particles

2007 ◽  
Vol 41 (4) ◽  
pp. 630-637 ◽  
Author(s):  
M. A. Nemykh ◽  
V. K. Novikov ◽  
A. M. Arutyunyan ◽  
P. V. Kalmykov ◽  
V. A. Drachev ◽  
...  
Keyword(s):  
Coat Protein ◽  
Structural Stability ◽  
Potato Virus ◽  
Potato Virus X ◽  
Virus Particles

scholarly journals Inhibition of RNA silencing by the coat protein of Pelargonium flower break virus: distinctions from closely related suppressors

2009 ◽  
Vol 90 (2) ◽  
pp. 519-525 ◽  
Author(s):  
Sandra Martínez-Turiño ◽  
Carmen Hernández
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Rna Silencing ◽  
Potato Virus X ◽  
Plant Viruses ◽  
Small Interfering Rnas ◽  
Silencing Suppression ◽  
Viral Suppressors

Viral-derived double-stranded RNAs (dsRNAs) activate RNA silencing, generating small interfering RNAs (siRNAs) which are incorporated into an RNA-induced silencing complex (RISC) that promotes homology-dependent degradation of cognate RNAs. To counteract this, plant viruses express RNA silencing suppressors. Here, we show that the coat protein (CP) of Pelargonium flower break virus (PFBV), a member of the genus Carmovirus, is able to efficiently inhibit RNA silencing. Interestingly, PFBV CP blocked both sense RNA- and dsRNA-triggered RNA silencing and did not preclude generation of siRNAs, which is in contrast with the abilities that have been reported for other carmoviral CPs. We have also found that PFBV CP can bind siRNAs and that this ability correlates with silencing suppression activity and enhancement of potato virus X pathogenicity. Collectively, the results indicate that PFBV CP inhibits RNA silencing by sequestering siRNAs and preventing their incorporation into a RISC, thus behaving similarly to unrelated viral suppressors but dissimilarly to orthologous ones.

scholarly journals Stability of recombinant plant viruses containing genes of unrelated plant viruses

2007 ◽  
Vol 88 (4) ◽  
pp. 1347-1355 ◽  
Author(s):  
Bong-Nam Chung ◽  
Tomas Canto ◽  
Peter Palukaitis
Keyword(s):  
Transgenic Plants ◽  
Transgenic Tobacco ◽  
Potato Virus ◽  
Tobacco Rattle Virus ◽  
Resistance Mechanism ◽  
Potato Virus X ◽  
Plant Viruses ◽  
Hybrid Plant ◽  
Expression Vectors ◽  
Post Inoculation

The stability of hybrid plant viruses that might arise by recombination in transgenic plants was examined using hybrid viruses derived from the viral expression vectors potato virus X (PVX) and tobacco rattle virus (TRV). The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was introduced into a vector derived from TRV RNA2 to generate TRV-NIb. All three viruses were unstable and most of the progeny viruses had lost the inserted sequences between 2 and 4 weeks post-inoculation. There was some variation in the rate of loss of part or all of the inserted sequence and the number of plants containing the deleted viruses, depending on the sequence, the host (Nicotiana tabacum vs Nicotiana benthamiana) or the vector, although none of these factors was associated consistently with the preferential loss of the inserted sequences. PVX-NIb was unable to accumulate in NIb-transgenic tobacco resistant to infection by PVY and also showed loss of the NIb insert from PVX-NIb in some NIb-transgenic tobacco plants susceptible to infection by PVY. These data indicate that such hybrid viruses, formed in resistant transgenic plants from a transgene and an unrelated virus, would be at a selective disadvantage, first by being targeted by the resistance mechanism and second by not being competitive with the parental virus.

scholarly journals Serological Identity of Potato Virus X (PVX) and PCR Characterization of Its Coat Protein (CP) Gene

2013 ◽  
Vol 2 (2) ◽  
pp. 92-96
Author(s):  
Qudsia Nosheen ◽  
Shahid Hameed ◽  
Sardar M. Mughal ◽  
Muhammad F. Abbas
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Crop ◽  
Potato Virus X ◽  
Datura Stramonium ◽  
Plant Viruses ◽  
Cp Gene ◽  
Antisense Primer ◽  
Specific Sense

Potato virus X (PVX) is among top ten most economically damaging plant viruses in the world and its increasing incidence is getting an alarming situation in potato crop of Pakistan. During two consecutive years (2010-11 and 2011-12), the incidence of PVX was recorded in potato fields at Rawalpindi, Islamabad, Faisalabad and Sahiwal. The samples were collected and subjected to Double Antibody Sandwiched (DAS) Enzyme Linked Immunosorbant Assay (ELISA) and average incidence of PVX was determined about 16.86% (OD405nm 1.38) during 2010-11 and 27.10% (OD405nm 0.479) in 2011-12. The infectivity of the virus was assayed through mechanical inoculation on Nicotiana tabacum cv. Samsun, N. rustica, Datura stramonium, Chenopodium sp. Gomphrena and Capsicum annuum producing local lesion, mosaic and mottling symptoms. Coat protein (CP) gene specific sense and antisense primer successfully amplified a 750bp fragments through Polymerase Chain Reaction (PCR) assay.

Characteristics of Artificial Virus-like Particles Assembled in vitro from Potato Virus X Coat Protein and Foreign Viral RNAs

Acta Naturae ◽  
2011 ◽  
Vol 3 (3) ◽  
pp. 40-46 ◽  
Author(s):  
M V Arkhipenko ◽  
E K Petrova ◽  
N A Nikitin ◽  
A D Protopopova ◽  
E V Dubrovin ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus X ◽  
Viral Rnas ◽  
Virus Like Particles ◽  
Artificial Virus

scholarly journals Translational Activation of Encapsidated Potato Virus X RNA by Coat Protein Phosphorylation

Virology ◽  
2001 ◽  
Vol 286 (2) ◽  
pp. 466-474 ◽  
Author(s):  
J.G. Atabekov ◽  
N.P. Rodionova ◽  
O.V. Karpova ◽  
S.V. Kozlovsky ◽  
V.K. Novikov ◽  
...  
Keyword(s):  
Coat Protein ◽  
Protein Phosphorylation ◽  
Potato Virus ◽  
Potato Virus X ◽  
Translational Activation

Stability of Potato virus X expression vectors is related to insert size: implications for replication models and risk assessment

2007 ◽  
Vol 16 (5) ◽  
pp. 587-597 ◽  
Author(s):  
Linda Avesani ◽  
Giampiero Marconi ◽  
Francesca Morandini ◽  
Emidio Albertini ◽  
Matteo Bruschetta ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Potato Virus ◽  
Potato Virus X ◽  
Expression Vectors ◽  
Insert Size
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