Post-transcriptional gene silencing and virus resistance in Nicotiana benthamiana expressing a Grapevine virus A minireplicon

2008 ◽  
Vol 18 (3) ◽  
pp. 331-345 ◽  
Author(s):  
Marina Brumin ◽  
Svetlana Stukalov ◽  
Sabrina Haviv ◽  
Mookkan Muruganantham ◽  
Yoni Moskovitz ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Virus Resistance ◽  
Nicotiana Benthamiana ◽  
Transcriptional Gene Silencing ◽  
Grapevine Virus ◽  
Grapevine Virus A ◽  
Post Transcriptional Gene Silencing

Grapevine virus A-mediated gene silencing in Nicotiana benthamiana and Vitis vinifera

2009 ◽  
Vol 155 (2) ◽  
pp. 167-174 ◽  
Author(s):  
Mookkan Muruganantham ◽  
Yoni Moskovitz ◽  
Sabrina Haviv ◽  
Tamar Horesh ◽  
Annie Fenigstein ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Vitis Vinifera ◽  
Nicotiana Benthamiana ◽  
Grapevine Virus ◽  
Grapevine Virus A

scholarly journals Virus-Induced Gene Silencing in Transgenic Plants Expressing the Minor Capsid Protein of Beet western yellows virus

2002 ◽  
Vol 15 (8) ◽  
pp. 799-807 ◽  
Author(s):  
V. Brault ◽  
S. Pfeffer ◽  
M. Erdinger ◽  
J. Mutterer ◽  
V. Ziegler-Graff
Keyword(s):  
Coat Protein ◽  
Gene Silencing ◽  
Nicotiana Benthamiana ◽  
Transcriptional Gene Silencing ◽  
Virus Induced Gene Silencing ◽  
Minor Capsid Protein ◽  
Beet Western Yellows Virus ◽  
Post Transcriptional Gene Silencing ◽  
Low Levels ◽  
Minor Coat Protein

Transgenic Nicotiana benthamiana expressing the minor coat protein P74 of the phloem-limited Beet western yellows virus (BWYV) exhibited an unusual spatial pattern of post-transcriptional gene silencing (PTGS) when infected with BWYV or related viruses. Following infection, transgenic P74 and its mRNA accumulated to only low levels, 21 to 23 nucleotide RNAs homologous to the transgene appeared, and the transgene DNA underwent methylation. The infecting viral RNA, however, was not subject to significant silencing but multiplied readily and produced P74 in the phloem tissues, although the P74 encoded by the transgene disappeared from the phloem as well as the nonvascular tissues.

A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance

Microbiology ◽  
2000 ◽  
Vol 81 (1) ◽  
pp. 235-242 ◽  
Author(s):  
Fuh-Jyh Jan ◽  
Carmen Fagoaga ◽  
Sheng-Zhi Pang ◽  
Dennis Gonsalves
Keyword(s):  
Transgenic Plants ◽  
Gene Silencing ◽  
Tobacco Mosaic Virus ◽  
Virus Resistance ◽  
N Gene ◽  
Transcriptional Gene Silencing ◽  
Viral Gene ◽  
Minimum Length ◽  
Post Transcriptional Gene Silencing ◽  
Multiple Virus

We showed previously that transgenic plants with the green fluorescent protein (GFP) gene fused to segments of the nucleocapsid (N) gene of tomato spotted wilt virus (TSWV) displayed post-transcriptional gene silencing of the GFP and N gene segments and resistance to TSWV. These results suggested that a chimeric transgene composed of viral gene segments might confer multiple virus resistance in transgenic plants. To test this hypothesis and to determine the minimum length of the N gene that could trans-inactivate the challenging TSWV, transgenic plants were developed that contained GFP fused with N gene segments of 24–453 bp. Progeny from these plants were challenged with: (i) a chimeric tobacco mosaic virus containing the GFP gene, (ii) a chimeric tobacco mosaic virus with GFP plus the N gene of TSWV and (iii) TSWV. A number of transgenic plants expressing the transgene with GFP fused to N gene segments from 110 to 453 bp in size were resistant to these viruses. Resistant plants exhibited post-transcriptional gene silencing. In contrast, all transgenic lines with transgenes consisting of GFP fused to N gene segments of 24 or 59 bp were susceptible to TSWV, even though the transgene was post-transcriptionally silenced. Thus, virus resistance and post-transcriptional gene silencing were uncoupled when the N gene segment was 59 bp or less. These results provide evidence that multiple virus resistance is possible through the simple strategy of linking viral gene segments to a silencer DNA such as GFP.

scholarly journals Fertile Hypomorphic ARGONAUTE (ago1) Mutants Impaired in Post-Transcriptional Gene Silencing and Virus Resistance

2002 ◽  
Vol 14 (3) ◽  
pp. 629-639 ◽  
Author(s):  
Jean-Benoit Morel ◽  
Christian Godon ◽  
Philippe Mourrain ◽  
Christophe Béclin ◽  
Stéphanie Boutet ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Virus Resistance ◽  
Transcriptional Gene Silencing ◽  
Post Transcriptional Gene Silencing

scholarly journals Soybean Mosaic Virus Resistance in Transgenic Soybean Caused by Post-transcriptional Gene Silencing

2007 ◽  
Vol 57 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Noriyuki Furutani ◽  
Noriko Yamagishi ◽  
Soh Hidaka ◽  
Yoshiaki Shizukawa ◽  
Seiji Kanematsu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Soybean Mosaic Virus ◽  
Transcriptional Gene Silencing ◽  
Transgenic Soybean ◽  
Post Transcriptional Gene Silencing
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