Efficient in vitro plant regeneration from seedling-derived explants and genetic stability analysis of regenerated plants of Simarouba glauca DC. by RAPD and ISSR markers

2016 ◽  
Vol 53 (1) ◽  
pp. 50-63 ◽  
Author(s):  
Madhavi Bramhanapalli ◽  
Latha Thogatabalija ◽  
Padmaja Gudipalli
Keyword(s):  
Stability Analysis ◽  
Plant Regeneration ◽  
Genetic Stability ◽  
Issr Markers ◽  
In Vitro Plant Regeneration ◽  
Regenerated Plants ◽  
In Vitro Plant ◽  
Simarouba Glauca ◽  
Rapd And Issr

Genetic stability using RAPD and ISSR markers in efficiently in vitro regenerated plants of Inula royleana DC

Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Regenerated Plants ◽  
Rapd And Issr

scholarly journals Assessment of Genetic Stability on In Vitro And Ex Vitro Plants of Ficus Carica Var. Black Jack Using Issr and Damd Markers

2021 ◽  
Author(s):  
Ankita Rajendra Parab ◽  
Chew Bee Lynn ◽  
Sreeramanan Subramaniam
Keyword(s):  
Stability Analysis ◽  
Genetic Stability ◽  
Similarity Index ◽  
Issr Markers ◽  
Ficus Carica ◽  
Ex Vitro ◽  
Regenerated Plants ◽  
Inter Simple Sequence Repeats ◽  
Dna Primers

Abstract In vitro propagation has been significant in producing a large number of genetically stable regenerated plants. Regenerated Ficus carica var. Black Jack plantlets were established using woody plant medium (WPM) supplemented with 20 µM 6-Benzylaminopurine (BAP) and 8 µM Indole-3-acetic acid (IAA) under different light treatments such as normal fluorescent white light (60 µmol.m− 2.s− 1), and four different LED spectra, white (400– 700nm), blue (440nm), red (660nm) and blue + red (440nm + 660nm). Genetic stability analysis was performed on the in vitro and ex vitro plants of Ficus carica var. Black Jack. Ten (10) primers of each ISSR and DAMD molecular marker were used to assess the genetic stability of the eight (8) samples of Ficus carica var. Black Jack, acquired over two years. The findings of this study revealed that inter simple sequence repeats (ISSR) and directed amplification of minisatellite DNA (DAMD) markers (DNA primers) are efficient in determining the polymorphism and monomorphism percentage among the in vitro and ex vitro samples of Ficus carica var. Black Jack. ISSR markers showed 97.87% of monomorphism whereas DAMD markers showed 100% monomorphism. Polymorphism of 2.13% was observed for the UBC840 ISSR – DNA primer which was negated under the genetic similarity index analysis for the eight samples. It is recommended that genetic stability analysis should be performed for long-term maintenance of micropropagated plants.

In vitro plant regeneration of caper (Capparis spinosa L.) from floral explants and genetic stability of regenerants

2011 ◽  
Vol 109 (2) ◽  
pp. 373-381 ◽  
Author(s):  
Angela Carra ◽  
Maurizio Sajeva ◽  
Loredana Abbate ◽  
Mirko Siragusa ◽  
Francesco Sottile ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Stability ◽  
In Vitro Plant Regeneration ◽  
Capparis Spinosa ◽  
In Vitro Plant ◽  
Floral Explants
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