scholarly journals Correction to: Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

2021 ◽  
Author(s):  
P. Srinivasan ◽  
H. David Raja ◽  
R. Tamilvanan
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Genetic Fidelity ◽  
Issr Markers ◽  
In Vitro Plant Regeneration ◽  
Fidelity Assessment ◽  
In Vitro Plant ◽  
Endemic Medicinal Plant ◽  
Rapd And Issr

scholarly journals Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 1000-1005 ◽  
Author(s):  
Ilse-Yazmín Arciniega-Carreón ◽  
Carmen Oliver-Salvador ◽  
María-Guadalupe Ramírez-Sotelo ◽  
Carlos Edmundo Salas
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Medicinal Plant ◽  
Metabolic Disorders ◽  
Naphthalene Acetic Acid ◽  
In Vitro Plant Regeneration ◽  
Shoot Production ◽  
In Vitro Plant ◽  
Indole 3 Acetic Acid

Ibervillea sonorae is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L−1 α-naphthalene-acetic acid (NAA), 0.5 mg·L−1 N6-benzyladenine (BA), and 1.0 mg·L−1 indole-3-acetic acid (IAA) successfully regenerated shoots in I. sonorae explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L−1 indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L−1 IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.

scholarly journals Efficient in vitro plant regeneration from leaf derived callus and genetic fidelity assessment of an endemic medicinal plant of Ranunculus wallichianus Wight & Arnn using RAPD and ISSR markers

2021 ◽  
Author(s):  
Srinivasan P ◽  
David Raja H ◽  
Tamilvanan R
Keyword(s):  
Callus Formation ◽  
Leaf Explants ◽  
Genetic Fidelity ◽  
Issr Markers ◽  
Ms Medium ◽  
Genetic Purity ◽  
Fidelity Assessment ◽  
Half Strength ◽  
Rapd And Issr

Abstract Ranunculus wallichianus is a medicinally important plant and an endemic species to Western Ghats of South India. An efficient and reliable indirect regeneration protocol system for R. wallichianus was developed from leaf explants in the present investigation. Leaf explants were cultured on both full-strength and half-strength MS (Murashige & Skoog) medium supplemented with different concentrations (1.0 mg L− 1 to 3.0 mg L− 1) of 2,4-D and NAA. Among the different concentrations tested, the highest percentage of yellowish green compact nodular callus formation was observed on half-strength MS medium with 2.0 mg L− 1 of 2, 4-D. Then, the in vitro raised organogenic callus was cultured on half strength MS medium containing various concentrations (1.0 mg L− 1 to 3.0 mg L− 1) of BA, KIN and TDZ with 0.5 mg L− 1 NAA and 10% CW for in vitro shoot regeneration. The highest percentage of regeneration response (97%) and maximum number of shoots formation (11.1 ± 0.13 shoots/culture with 9.2 ± 0.35 cm mean shoot length) were obtained from MS medium containing 2.5 mg L− 1 BA with 0.5 mg L− 1 NAA and 10% CW. The well elongated in vitro raised shoots were rooted in half strength MS medium with 2.5 mg L− 1 IBA + 250 mg L− 1 activated charcoal shows high frequency of root formation. The well rooted plantlets were successfully hardened and acclimatized with the survival rate of 94%. Clonal fidelity of in vitro raised plantlets was assessed by using DNA based RAPD and ISSR molecular markers. The total of 56 and 47 monomorphic bands were obtained from RAPD and ISSR markers respectively. This present in vitro propagation protocol system could be an effective for the conservation of R. wallichianus with their genetic purity and its further investigations.

scholarly journals In vitro plant regeneration of Coccinea cordifolia (Linn.) Cogn., an anti-diabetic medicinal plant

2012 ◽  
Vol 47 (2) ◽  
pp. 187-190
Author(s):  
Chapol Kumar Roy ◽  
John Liton Munshia ◽  
Nadira Begum ◽  
Rahima Khatun ◽  
AKM Sayeed Hassanb
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Half Strength ◽  
In Vitro Plant ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro plant regeneration of a popularly used anti-diabetic medicinal plant, Coccinea cordifolia (Linn.) Cogn.(Cucurbitaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 88.2% of nodal explants responded to produce maximum number (6.2 ± 0.58) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. The survival rate of regenerated plantlets was 85%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11450   Bangladesh J. Sci. Ind. Res. 47(2), 187-190, 2012  

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