Rapid in vitro propagation of Saussurea lappa, an endangered medicinal plant, through multiple shoot cultures

1997 ◽  
Vol 33 (2) ◽  
pp. 128-130 ◽  
Author(s):  
T. Sudhakar Johnson ◽  
S. Badari Narayan ◽  
D. B. A. Narayana
1979 ◽  
Vol 59 (4) ◽  
pp. 1025-1029 ◽  
Author(s):  
W. DAVID LANE

A procedure was developed to produce large numbers of plants from multiple shoot, cultures which were obtained from shoot apices 1–5 mm long of Spirea bumalda ’Anthony Waterer’ and Prunus cistena grown on Murashige and Skoog (MS) medium containing 5 μM benzyladenine. Shoot production, harvested every 3–4 wk, yielded 300–400 shoots of Spirea and approximately 35 of Prunus per cycle. The salt concentration of MS inhibited rooting was reduced by half. Liquid media usually resulted in greater percent rooting than agar. Although there was some rooting of both species in medium without naphthalenacetic acid, this auxin increased rooting of both species with optima at about 0.1 μM for Spirea and 1 μM for Prunus.


2012 ◽  
Vol 18 (3) ◽  
pp. 281-286 ◽  
Author(s):  
Sompop Prathanturarug ◽  
Rungthipwan Pheakkoet ◽  
Thaya Jenjittikul ◽  
Wongsatit Chuakul ◽  
Promchit Saralamp

2019 ◽  
Vol 100 (3) ◽  
pp. 209-217
Author(s):  
Varnika Chaudhary ◽  
Shalini Singh ◽  
Rohit Sharma ◽  
Ajay Singh ◽  
Nishesh Sharma

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Ashok Ahuja ◽  
Devinder Kaur ◽  
Mallubhotla Sharada ◽  
Arun Kumar ◽  
Krishan Avtar Suri ◽  
...  

Phytochemical investigations of multiple shoot cultures of selected accessions AGB002 and AGB025 of Withania somnifera. established in vitro utilizing shoot tip apices cultured on Murashige and Skoog's medium supplemented with BAP (1 mg/L) have been carried out. This has lead to isolation of four glycowithanolides viz. Withanoside IV (WSG-3), Withanoside VI (WSG-3A), Physagulin D (WSG-P) and Withastraronolide (WSC-O). The structures of these have been confirmed on the basis of spectroscopic data. Multiple shoot cultures could be an alternative renewable resource for production of these biologically active molecules.


2018 ◽  
Vol 135 (3) ◽  
pp. 559-563 ◽  
Author(s):  
Wen-Tao Zhu ◽  
Jiu-Zhen Du ◽  
Hong-Bing Sun ◽  
Shun-Yuan Jiang ◽  
Xue-Jun Chen ◽  
...  

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