Cloning and prokaryotic expression of translationally controlled tumor protein (TaTCTP) gene from wheat and preparation of antiserum

2011 ◽  
Vol 5 (4) ◽  
pp. 473-478 ◽  
Author(s):  
Lifeng Zhang ◽  
Aihua Yan ◽  
Dong Tian ◽  
Shengfang Han ◽  
Dongmei Wang
Keyword(s):  
Prokaryotic Expression ◽  
Translationally Controlled Tumor Protein ◽  
Preparation Of Antiserum

scholarly journals MOLECULAR CLONING, PROKARYOTIC EXPRESSION AND PREPARATION OF ANTISERUM AGAINST LILY SYMPTOMLESS VIRUS TGB1 PROTEIN

2017 ◽  
Vol 7 (1) ◽  
pp. 986-990
Author(s):  
Pinsan Xu ◽  
Xinlei Wang ◽  
Zhengyao Zhang
Keyword(s):  
Prokaryotic Expression ◽  
Triple Gene Block ◽  
Rapid Test ◽  
Coli Strain ◽  
Functional Study ◽  
Rt Pcr ◽  
Lily Symptomless Virus ◽  
Gene Block ◽  
Prokaryotic Expression Vector ◽  
Preparation Of Antiserum

 The triple gene block gene TGB1 was amplified by RT-PCR from lily leaves infected with Lily symptomless virus and cloned into prokaryotic expression vector pET-28a(+). The recombinant vector was transformed into Escherichia coli strain BL21 (DE3). On induction with isopropyl β-D-1-thiogalactopyranoside,TGB1 protein was highly expressed and the molecular weight was 29 kDa (including a His-tag-containing fusion). After protein purification by Ni2+-NTA affinity chromatography, a polyclonal antibody against TGB1 was raised in mouse. Western blot analysis showed that the antiserum reacted specially with the TGB1 protein of LSV. ELISA and RT-PCR confirmed that the antiserum reacted specially with lily leaves infected with LSV, and can be used for a rapid test for LSV. The antibody produced in this work may be used for future immunohistochemistry and functional study of TGB1.

Cloning and prokaryotic expression of TaE3 from wheat and preparation of antiserum

2011 ◽  
Vol 5 (4) ◽  
pp. 437-442 ◽  
Author(s):  
Yunwei Zhang ◽  
Xiang Gao ◽  
Shengfang Han ◽  
Dongmei Wang
Keyword(s):  
Prokaryotic Expression ◽  
Preparation Of Antiserum

Cloning and prokaryotic expression in TaCaM2-3 of wheat and preparation of antiserum

2010 ◽  
Vol 4 (3) ◽  
pp. 317-322 ◽  
Author(s):  
Wei Liu ◽  
Aihua Yan ◽  
Chunyan Hou ◽  
Dongmei Wang
Keyword(s):  
Prokaryotic Expression ◽  
Preparation Of Antiserum

Cloning, Prokaryotic Expression, and Functional Testing of a γ-Gliadin Gene from Wheat Cultivar Shaan 253

2011 ◽  
Vol 37 (1) ◽  
pp. 79-86
Author(s):  
Ming-Xia WANG ◽  
Xiang GAO ◽  
Qi-Jiao CHEN ◽  
Jian DONG ◽  
Wan-Chun ZHAO ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Wheat Cultivar ◽  
Functional Testing

CLONING AND PROKARYOTIC EXPRESSION OF HSP70 cDNA FROM PLANARIAN DUGESIA JAPONICA

2010 ◽  
Vol 36 (2) ◽  
pp. 317-322
Author(s):  
Ke-Xue MA ◽  
Guang-Wen CHEN ◽  
Hao LOU ◽  
Li-Na FEI
Keyword(s):  
Prokaryotic Expression ◽  
Dugesia Japonica

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis
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