An improved micropropagation of Spilanthes acmella L. through transverse thin cell layer culture

2009 ◽  
Vol 31 (4) ◽  
pp. 693-698 ◽  
Author(s):  
Shashi Kant Singh ◽  
Manoj K. Rai ◽  
Pooja Asthana ◽  
L. Sahoo
Keyword(s):  
Cell Layer ◽  
Thin Cell Layer ◽  
Transverse Thin Cell Layer ◽  
Spilanthes Acmella ◽  
Thin Cell Layer Culture

Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture

2007 ◽  
Vol 111 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Duong Tan Nhut ◽  
Truong Thi Thuy An ◽  
Nguyen Thi Dieu Huong ◽  
Nguyen Trinh Don ◽  
Nguyen Thanh Hai ◽  
...  
Keyword(s):  
Culture Medium ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Gerbera Jamesonii ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture

Shoot induction and regeneration using internodal transverse thin cell layer culture in Sesamum indicum L.

2010 ◽  
Vol 4 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Banani Chattopadhyaya ◽  
Joydeep Banerjee ◽  
Asitava Basu ◽  
Soumitra K. Sen ◽  
Mrinal K. Maiti
Keyword(s):  
Cell Layer ◽  
Sesamum Indicum ◽  
Thin Cell Layer ◽  
Shoot Induction ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture

scholarly journals STUDY ON MORPHOGENESIS OF BLACK PEPPER (PIPER NIGRUM L.) IN LEAF THIN CELL LAYER CULTURE

2009 ◽  
Vol 12 (17) ◽  
pp. 106-110
Author(s):  
Giap Dang Do ◽  
Du Xuan Thai ◽  
Thuyen Thi Ai Doan
Keyword(s):  
Apical Meristem ◽  
Cell Layer ◽  
Histological Study ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Thin Cell Layer ◽  
Transverse Thin Cell Layer ◽  
Undifferentiated Cells ◽  
Thin Cell Layer Culture ◽  
Xylem Elements

A histological study of callus regeneration of black pepper using leaf transverse thin cell layer (TCL) explants was accomplished. The undifferentiated cells culture of callus were found to differentiate into vascular nodules called meristemoids, which then develop into xylem elements, especially tracheids. Culturing in the shooting medium, these nodules differentiated into shoot apical meristem.

Micropropagation of Rosa damascena Mill. through transverse thin cell layer culture

2012 ◽  
Vol 2 (4) ◽  
pp. 184-189
Author(s):  
Kavita Kshirsagar ◽  
V. J. Braganza
Keyword(s):  
Shoot Regeneration ◽  
Large Scale ◽  
Cell Layer ◽  
In Vitro Regeneration ◽  
Rosa Damascena ◽  
Ms Medium ◽  
Thin Cell Layer ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture

A basic factor underlying the success of large‐scale micropropagation and genetic transformation of any plant species is regeneration. In order to regenerate propagules of Rosa damascena Mill. on a large scale, an efficient and improved in vitro propagation system has been established using transverse thin cell layer culture (tTCL). By optimizing the position of the tissue and applying an improved selection procedure, in vitro shoots were elongated in 8 weeks of culture. Modified Murashige and Skoog (1962)(MS) medium fortified with 4.0 mg l‐1 6‐benzylaminopurine (BAP) and 0.4mg l‐1 anaphthalene acetic acid (NAA) gave optimal shoot regeneration. The explant was inoculated on this medium in the upright position and exhibited a high frequency of shoot regeneration (~96.66%), and it also gave the highest number of shoots (22.33/explant). The horizontally placed explant on an average 7.66 shoots/explant. Our experiments indicate that explant orientation strongly influences the organogenesis response. The frequency of shoot initiation and the number of multiple shoots produced from each explant were significantly dependent on the plant source, concentration of plant growth regulators and the orientation of the explants and contributed significantly to in vitro regeneration. Rooting of well developed shoots was achieved on hormone free ¼ strength MS medium with 4% sucrose.

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