High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl.

2007 ◽  
Vol 90 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Peng Zhao ◽  
Wei Wang ◽  
Fo-Sheng Feng ◽  
Fei Wu ◽  
Zhong-Qi Yang ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture ◽  
Dendrobium Candidum

Micropropagation of Rosa damascena Mill. through transverse thin cell layer culture

2012 ◽  
Vol 2 (4) ◽  
pp. 184-189
Author(s):  
Kavita Kshirsagar ◽  
V. J. Braganza
Keyword(s):  
Shoot Regeneration ◽  
Large Scale ◽  
Cell Layer ◽  
In Vitro Regeneration ◽  
Rosa Damascena ◽  
Ms Medium ◽  
Thin Cell Layer ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture

A basic factor underlying the success of large‐scale micropropagation and genetic transformation of any plant species is regeneration. In order to regenerate propagules of Rosa damascena Mill. on a large scale, an efficient and improved in vitro propagation system has been established using transverse thin cell layer culture (tTCL). By optimizing the position of the tissue and applying an improved selection procedure, in vitro shoots were elongated in 8 weeks of culture. Modified Murashige and Skoog (1962)(MS) medium fortified with 4.0 mg l‐1 6‐benzylaminopurine (BAP) and 0.4mg l‐1 anaphthalene acetic acid (NAA) gave optimal shoot regeneration. The explant was inoculated on this medium in the upright position and exhibited a high frequency of shoot regeneration (~96.66%), and it also gave the highest number of shoots (22.33/explant). The horizontally placed explant on an average 7.66 shoots/explant. Our experiments indicate that explant orientation strongly influences the organogenesis response. The frequency of shoot initiation and the number of multiple shoots produced from each explant were significantly dependent on the plant source, concentration of plant growth regulators and the orientation of the explants and contributed significantly to in vitro regeneration. Rooting of well developed shoots was achieved on hormone free ¼ strength MS medium with 4% sucrose.

scholarly journals High Frequency Plant Regeneration System from Transverse Thin Cell Layer Section of In vitro Derived ‘Nadia’ Ginger Microrhizome

2014 ◽  
Vol 6 (1) ◽  
pp. 85-91
Author(s):  
Dikash Singh THINGBAIJAM ◽  
Devi Sunitibala HUIDROM
Keyword(s):  
High Frequency ◽  
Somatic Embryos ◽  
Cell Layer ◽  
Sucrose Concentration ◽  
Dichlorophenoxyacetic Acid ◽  
Thin Cell Layer ◽  
Regeneration System ◽  
Transverse Thin Cell Layer ◽  
2,4 Dichlorophenoxyacetic Acid

An efficient and reproducible procedure is outlined for rapid in vitro multiplication of Zingiber officinale var. ‘Nadia’ through high frequency shoot proliferation from transverse thin cell layer (tTCL) sections of in vitro derived microrhizome. In vitro derived microrhizome of size 500 μm in thickness was used as initial explants for induction of somatic embryos. Among the different phytohormones tested, tTCL explants shows maximum calli proliferation in medium containing 2 mg/L 2,4-Dichlorophenoxyacetic acid (88.30±0.11%). Reduced concentration of 2,4 Dichlorophenoxyacetic acid was supplemented with different cytokinins for regeneration of callus. Among the different medium tested, optimum redifferentiation of somatic embryos were observed in medium containing 0.2 mg/L 2,4 Dichlorophenoxyacetic acid and 6.0 mg/L BAP (141.08±0.25). Clump of regenerated plantlets were further subculture and transfer into microrhizome inducing medium containing high sucrose concentration (8%). Plantlets with well developed microrhizome were successfully acclimatized and eventually transferred to the field. The application of studying embryo section for regeneration of plants might be useful alternative to ginger improvement programme. Histological analysis showed formation of somatic embryos and regenerated adventitious shoot.

Effect of genotype, explant size, position, and culture medium on shoot generation of Gerbera jamesonii by receptacle transverse thin cell layer culture

2007 ◽  
Vol 111 (2) ◽  
pp. 146-151 ◽  
Author(s):  
Duong Tan Nhut ◽  
Truong Thi Thuy An ◽  
Nguyen Thi Dieu Huong ◽  
Nguyen Trinh Don ◽  
Nguyen Thanh Hai ◽  
...  
Keyword(s):  
Culture Medium ◽  
Cell Layer ◽  
Thin Cell Layer ◽  
Gerbera Jamesonii ◽  
Transverse Thin Cell Layer ◽  
Thin Cell Layer Culture
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