Synergistic Activity of Phenolic Compounds of Some Plants Against Bacteria

Medicinal plants produce great groups of secondary metabolites which are essential for medicine purpose, one of them phenolic compounds, antimicrobial activity of phenolic compounds which derivative from plants has been examined for several years. The phenolic extracts of Sesamum indicum and Pimpinella anisum seeds have antibacterial action against Gram positive (Staphylococcus aureus) and Gram-negative bacteria (Escherichia coli), (Acinetobacter baumannii), and (Pseudomonas aeruginosa) (Proteus mirabilis). The current findings show that the synergistic impact of phenolic extracts from S. indicum and P. anisum is active against a variety of pathogenic bacteria, and that the synergistic effect for two plants is more antibacterial than phenolic extracts from one plant. The results indicated Gram- negative (P. aeruginosa) more effected by plants, than Gram-negative (S. aureus) which have the lower effects. The results of HPLC indicated Sesame (S. indicum) have total concentration of phenolic compounds was (1313.7 µg/ml) higher than total concentration of phenolic compounds of Anise (P. anisum) (220.991 µg/ml), and have varied types of phenolic compounds were Pyrogallol, Gallic acid, Rutin, Kaempferol, Cinnamaldehyde, Qurctin, Eugenol, Lignan with different concentration. From this study may be conclusion Synergistic effect for two plants have more antibacterial than phenolic extracts of one plant, and Sesame (S. indicum) have higher antimicrobial activity than Anise (P. anisum).

DANCR Mediates the Rescuing Effects of Sesamin on Postmenopausal Osteoporosis Treatment via Orchestrating Osteogenesis and Osteoclastogenesis

As one of the leading causes of bone fracture in postmenopausal women and in older men, osteoporosis worldwide is attracting more attention in recent decades. Osteoporosis is a common disease mainly resulting from an imbalance of bone formation and bone resorption. Pharmaceutically active compounds that both activate osteogenesis, while repressing osteoclastogenesis hold the potential of being therapeutic medications for osteoporosis treatment. In the present study, sesamin, a bioactive ingredient derived from the seed of Sesamum Indicum, was screened out from a bioactive compound library and shown to exhibit dual-regulating functions on these two processes. Sesamin was demonstrated to promote osteogenesis by upregulating Wnt/β-catenin, while repressing osteoclastogenesis via downregulating NF-κB signaling . Furthermore, DANCR was found to be the key regulator in sesamin-mediated bone formation and resorption . In an ovariectomy (OVX)-induced osteoporotic mouse model, sesamin could rescue OVX-induced bone loss and impairment. The increased serum level of DANCR caused by OVX was also downregulated upon sesamin treatment. In conclusion, our results demonstrate that sesamin plays a dual-functional role in both osteogenesis activation and osteoclastogenesis de-activation in a DANCR-dependent manner, suggesting that it may be a possible medication candidate for osteoporotic patients with elevated DNACR expression levels.

Comprehensive Analysis of SRO Gene Family in Sesamum indicum (L.) Reveals Its Association with Abiotic Stress Responses

SIMILAR TO RCD-ONEs (SROs) comprise a small plant-specific gene family which play important roles in regulating numerous growth and developmental processes and responses to environmental stresses. However, knowledge of SROs in sesame (Sesamum indicum L.) is limited. In this study, four SRO genes were identified in the sesame genome. Phylogenetic analysis showed that 64 SROs from 10 plant species were divided into two groups (Group I and II). Transcriptome data revealed different expression patterns of SiSROs over various tissues. Expression analysis showed that Group II SROs, especially SiSRO2b, exhibited a stronger response to various abiotic stresses and phytohormones than those in Group I, implying their crucial roles in response to environmental stimulus and hormone signals. In addition, the co-expression network and protein-protein interaction network indicated that SiSROs are associated with a wide range of stress responses. Moreover, transgenic yeast harboring SiSRO2b showed improved tolerance to salt, osmotic and oxidative stress, indicating SiSRO2b could confer multiple tolerances to transgenic yeast. Taken together, this study not only lays a foundation for further functional dissection of the SiSRO gene family, but also provides valuable gene candidates for genetic improvement of abiotic stress tolerance in sesame.